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Post-calving umbilical cord tissue offcut: A potential source for the isolation of bovine mesenchymal stem cells

BACKGROUND AND AIM: Veterinary health care is an emergent area in animal sciences and innovative therapeutic approaches happen to be imperative in the present days. In view of the importance of cattle health and production, it is necessary to take up contemporary approach of stem cell therapy in thi...

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Autores principales: Debbarma, Parishma, Mondal, Tanmay, Manna, Camelia, Kumar, Kuldeep, Mukherjee, Joydip, Das, Bikash Chandra, Bag, Sadhan, Das, Kinsuk
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Veterinary World 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7811551/
https://www.ncbi.nlm.nih.gov/pubmed/33487997
http://dx.doi.org/10.14202/vetworld.2020.2772-2779
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author Debbarma, Parishma
Mondal, Tanmay
Manna, Camelia
Kumar, Kuldeep
Mukherjee, Joydip
Das, Bikash Chandra
Bag, Sadhan
Das, Kinsuk
author_facet Debbarma, Parishma
Mondal, Tanmay
Manna, Camelia
Kumar, Kuldeep
Mukherjee, Joydip
Das, Bikash Chandra
Bag, Sadhan
Das, Kinsuk
author_sort Debbarma, Parishma
collection PubMed
description BACKGROUND AND AIM: Veterinary health care is an emergent area in animal sciences and innovative therapeutic approaches happen to be imperative in the present days. In view of the importance of cattle health and production, it is necessary to take up contemporary approach of stem cell therapy in this sector also. This study aimed to standardize an explant culture method of bovine umbilical tissue offcut to isolate mesenchymal stem cells (MSCs) because considerable efforts are required for ensuring easy accessibility and availability of MSCs in bulk quantity, as well as in establishing and characterizing the cell lines. MATERIALS AND METHODS: The umbilical cord (UC) tissue matrix offcut was collected after calving. A simplified in vitro cell isolation technique was followed to collect the emerged out cells from the explants of UC. Further, we expanded these isolated cells in vitro, observed its growth kinetics, and characterized to confirm as per the criterion of bovine MSCs. RESULTS: A considerable exponential growth rate of the UC-derived cells was noticed. In addition to their confirmation as MSCs, the cells also exhibited plastic adherent property and maintained the spindle-shaped morphology throughout the in vitro culture. The cultured cells were found positive MSC-specific surface markers CD105, CD90, and CD73 and were negative for hematopoietic cell marker CD45. Cytochemical studies revealed the ability of the cells to differentiate into osteogenic, chondrogenic, and adipogenic lineages. CONCLUSION: This simplified method of isolation and culture of bovine multipotent MSCs from the UC offcut collected after calving could be extrapolated for the greater availability of the cells for prospective therapeutic applications.
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spelling pubmed-78115512021-01-22 Post-calving umbilical cord tissue offcut: A potential source for the isolation of bovine mesenchymal stem cells Debbarma, Parishma Mondal, Tanmay Manna, Camelia Kumar, Kuldeep Mukherjee, Joydip Das, Bikash Chandra Bag, Sadhan Das, Kinsuk Vet World Research Article BACKGROUND AND AIM: Veterinary health care is an emergent area in animal sciences and innovative therapeutic approaches happen to be imperative in the present days. In view of the importance of cattle health and production, it is necessary to take up contemporary approach of stem cell therapy in this sector also. This study aimed to standardize an explant culture method of bovine umbilical tissue offcut to isolate mesenchymal stem cells (MSCs) because considerable efforts are required for ensuring easy accessibility and availability of MSCs in bulk quantity, as well as in establishing and characterizing the cell lines. MATERIALS AND METHODS: The umbilical cord (UC) tissue matrix offcut was collected after calving. A simplified in vitro cell isolation technique was followed to collect the emerged out cells from the explants of UC. Further, we expanded these isolated cells in vitro, observed its growth kinetics, and characterized to confirm as per the criterion of bovine MSCs. RESULTS: A considerable exponential growth rate of the UC-derived cells was noticed. In addition to their confirmation as MSCs, the cells also exhibited plastic adherent property and maintained the spindle-shaped morphology throughout the in vitro culture. The cultured cells were found positive MSC-specific surface markers CD105, CD90, and CD73 and were negative for hematopoietic cell marker CD45. Cytochemical studies revealed the ability of the cells to differentiate into osteogenic, chondrogenic, and adipogenic lineages. CONCLUSION: This simplified method of isolation and culture of bovine multipotent MSCs from the UC offcut collected after calving could be extrapolated for the greater availability of the cells for prospective therapeutic applications. Veterinary World 2020-12 2020-12-25 /pmc/articles/PMC7811551/ /pubmed/33487997 http://dx.doi.org/10.14202/vetworld.2020.2772-2779 Text en Copyright: © Debbarma, et al. http://creativecommons.org/licenses/by/4.0 Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Debbarma, Parishma
Mondal, Tanmay
Manna, Camelia
Kumar, Kuldeep
Mukherjee, Joydip
Das, Bikash Chandra
Bag, Sadhan
Das, Kinsuk
Post-calving umbilical cord tissue offcut: A potential source for the isolation of bovine mesenchymal stem cells
title Post-calving umbilical cord tissue offcut: A potential source for the isolation of bovine mesenchymal stem cells
title_full Post-calving umbilical cord tissue offcut: A potential source for the isolation of bovine mesenchymal stem cells
title_fullStr Post-calving umbilical cord tissue offcut: A potential source for the isolation of bovine mesenchymal stem cells
title_full_unstemmed Post-calving umbilical cord tissue offcut: A potential source for the isolation of bovine mesenchymal stem cells
title_short Post-calving umbilical cord tissue offcut: A potential source for the isolation of bovine mesenchymal stem cells
title_sort post-calving umbilical cord tissue offcut: a potential source for the isolation of bovine mesenchymal stem cells
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7811551/
https://www.ncbi.nlm.nih.gov/pubmed/33487997
http://dx.doi.org/10.14202/vetworld.2020.2772-2779
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