Cargando…
Post-calving umbilical cord tissue offcut: A potential source for the isolation of bovine mesenchymal stem cells
BACKGROUND AND AIM: Veterinary health care is an emergent area in animal sciences and innovative therapeutic approaches happen to be imperative in the present days. In view of the importance of cattle health and production, it is necessary to take up contemporary approach of stem cell therapy in thi...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Veterinary World
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7811551/ https://www.ncbi.nlm.nih.gov/pubmed/33487997 http://dx.doi.org/10.14202/vetworld.2020.2772-2779 |
_version_ | 1783637524687618048 |
---|---|
author | Debbarma, Parishma Mondal, Tanmay Manna, Camelia Kumar, Kuldeep Mukherjee, Joydip Das, Bikash Chandra Bag, Sadhan Das, Kinsuk |
author_facet | Debbarma, Parishma Mondal, Tanmay Manna, Camelia Kumar, Kuldeep Mukherjee, Joydip Das, Bikash Chandra Bag, Sadhan Das, Kinsuk |
author_sort | Debbarma, Parishma |
collection | PubMed |
description | BACKGROUND AND AIM: Veterinary health care is an emergent area in animal sciences and innovative therapeutic approaches happen to be imperative in the present days. In view of the importance of cattle health and production, it is necessary to take up contemporary approach of stem cell therapy in this sector also. This study aimed to standardize an explant culture method of bovine umbilical tissue offcut to isolate mesenchymal stem cells (MSCs) because considerable efforts are required for ensuring easy accessibility and availability of MSCs in bulk quantity, as well as in establishing and characterizing the cell lines. MATERIALS AND METHODS: The umbilical cord (UC) tissue matrix offcut was collected after calving. A simplified in vitro cell isolation technique was followed to collect the emerged out cells from the explants of UC. Further, we expanded these isolated cells in vitro, observed its growth kinetics, and characterized to confirm as per the criterion of bovine MSCs. RESULTS: A considerable exponential growth rate of the UC-derived cells was noticed. In addition to their confirmation as MSCs, the cells also exhibited plastic adherent property and maintained the spindle-shaped morphology throughout the in vitro culture. The cultured cells were found positive MSC-specific surface markers CD105, CD90, and CD73 and were negative for hematopoietic cell marker CD45. Cytochemical studies revealed the ability of the cells to differentiate into osteogenic, chondrogenic, and adipogenic lineages. CONCLUSION: This simplified method of isolation and culture of bovine multipotent MSCs from the UC offcut collected after calving could be extrapolated for the greater availability of the cells for prospective therapeutic applications. |
format | Online Article Text |
id | pubmed-7811551 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Veterinary World |
record_format | MEDLINE/PubMed |
spelling | pubmed-78115512021-01-22 Post-calving umbilical cord tissue offcut: A potential source for the isolation of bovine mesenchymal stem cells Debbarma, Parishma Mondal, Tanmay Manna, Camelia Kumar, Kuldeep Mukherjee, Joydip Das, Bikash Chandra Bag, Sadhan Das, Kinsuk Vet World Research Article BACKGROUND AND AIM: Veterinary health care is an emergent area in animal sciences and innovative therapeutic approaches happen to be imperative in the present days. In view of the importance of cattle health and production, it is necessary to take up contemporary approach of stem cell therapy in this sector also. This study aimed to standardize an explant culture method of bovine umbilical tissue offcut to isolate mesenchymal stem cells (MSCs) because considerable efforts are required for ensuring easy accessibility and availability of MSCs in bulk quantity, as well as in establishing and characterizing the cell lines. MATERIALS AND METHODS: The umbilical cord (UC) tissue matrix offcut was collected after calving. A simplified in vitro cell isolation technique was followed to collect the emerged out cells from the explants of UC. Further, we expanded these isolated cells in vitro, observed its growth kinetics, and characterized to confirm as per the criterion of bovine MSCs. RESULTS: A considerable exponential growth rate of the UC-derived cells was noticed. In addition to their confirmation as MSCs, the cells also exhibited plastic adherent property and maintained the spindle-shaped morphology throughout the in vitro culture. The cultured cells were found positive MSC-specific surface markers CD105, CD90, and CD73 and were negative for hematopoietic cell marker CD45. Cytochemical studies revealed the ability of the cells to differentiate into osteogenic, chondrogenic, and adipogenic lineages. CONCLUSION: This simplified method of isolation and culture of bovine multipotent MSCs from the UC offcut collected after calving could be extrapolated for the greater availability of the cells for prospective therapeutic applications. Veterinary World 2020-12 2020-12-25 /pmc/articles/PMC7811551/ /pubmed/33487997 http://dx.doi.org/10.14202/vetworld.2020.2772-2779 Text en Copyright: © Debbarma, et al. http://creativecommons.org/licenses/by/4.0 Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Article Debbarma, Parishma Mondal, Tanmay Manna, Camelia Kumar, Kuldeep Mukherjee, Joydip Das, Bikash Chandra Bag, Sadhan Das, Kinsuk Post-calving umbilical cord tissue offcut: A potential source for the isolation of bovine mesenchymal stem cells |
title | Post-calving umbilical cord tissue offcut: A potential source for the isolation of bovine mesenchymal stem cells |
title_full | Post-calving umbilical cord tissue offcut: A potential source for the isolation of bovine mesenchymal stem cells |
title_fullStr | Post-calving umbilical cord tissue offcut: A potential source for the isolation of bovine mesenchymal stem cells |
title_full_unstemmed | Post-calving umbilical cord tissue offcut: A potential source for the isolation of bovine mesenchymal stem cells |
title_short | Post-calving umbilical cord tissue offcut: A potential source for the isolation of bovine mesenchymal stem cells |
title_sort | post-calving umbilical cord tissue offcut: a potential source for the isolation of bovine mesenchymal stem cells |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7811551/ https://www.ncbi.nlm.nih.gov/pubmed/33487997 http://dx.doi.org/10.14202/vetworld.2020.2772-2779 |
work_keys_str_mv | AT debbarmaparishma postcalvingumbilicalcordtissueoffcutapotentialsourcefortheisolationofbovinemesenchymalstemcells AT mondaltanmay postcalvingumbilicalcordtissueoffcutapotentialsourcefortheisolationofbovinemesenchymalstemcells AT mannacamelia postcalvingumbilicalcordtissueoffcutapotentialsourcefortheisolationofbovinemesenchymalstemcells AT kumarkuldeep postcalvingumbilicalcordtissueoffcutapotentialsourcefortheisolationofbovinemesenchymalstemcells AT mukherjeejoydip postcalvingumbilicalcordtissueoffcutapotentialsourcefortheisolationofbovinemesenchymalstemcells AT dasbikashchandra postcalvingumbilicalcordtissueoffcutapotentialsourcefortheisolationofbovinemesenchymalstemcells AT bagsadhan postcalvingumbilicalcordtissueoffcutapotentialsourcefortheisolationofbovinemesenchymalstemcells AT daskinsuk postcalvingumbilicalcordtissueoffcutapotentialsourcefortheisolationofbovinemesenchymalstemcells |