Cargando…

Isolation, molecular identification, and pathological lesions of Saprolegnia spp. isolated from common carp, Cyprinus carpio in floating cages in Mosul, Iraq

BACKGROUND AND AIM: Saprolegniasis is a fungal disease that infects freshwater fish. The condition is characterized by a cotton-like appearance in the gills and body. This study aimed to isolate Saprolegnia from common carp, Cyprinus carpio, raised in a floating cage in Wana district, Mosul, Iraq. M...

Descripción completa

Detalles Bibliográficos
Autores principales: Ali, Fawwaz Fadhil, AL-Taee, Shahbaa Khalil, AL-Jumaa, Zahraa Mustafa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Veterinary World 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7811552/
https://www.ncbi.nlm.nih.gov/pubmed/33487995
http://dx.doi.org/10.14202/vetworld.2020.2759-2764
_version_ 1783637524922499072
author Ali, Fawwaz Fadhil
AL-Taee, Shahbaa Khalil
AL-Jumaa, Zahraa Mustafa
author_facet Ali, Fawwaz Fadhil
AL-Taee, Shahbaa Khalil
AL-Jumaa, Zahraa Mustafa
author_sort Ali, Fawwaz Fadhil
collection PubMed
description BACKGROUND AND AIM: Saprolegniasis is a fungal disease that infects freshwater fish. The condition is characterized by a cotton-like appearance in the gills and body. This study aimed to isolate Saprolegnia from common carp, Cyprinus carpio, raised in a floating cage in Wana district, Mosul, Iraq. MATERIALS AND METHODS: Samples were collected from 15 infected fish and examined microbiologically, molecularly, and histopathologically. Saprolegnia DNA was extracted which was amplified using universal primers give a 540 bp DNA fragment, and gill and muscle tissue were also examined for histopathological changes. RESULTS: Isolated colonies of Saprolegnia were characterized by a circular, white cottony appearance with long hair. Lactophenol staining demonstrated hyphae as branched non-septate, transparent masses. The genomic DNA of isolates was consistent with Saprolegnia spp. The infected tissue samples showed variable pathology in gills. Severe hemorrhage and edema were observed in primary gill filaments with hyperplasia in epithelial cells and infusion in secondary gill filaments. Hyphae of Saprolegnia were seen between necrotic and edematous myofiber with inflammatory cells infiltration. CONCLUSION: Saprolegnia can cause economic impacts through lethal infection of fish. Clinical signs of Saprolegnia infection were confirmed molecularly and microscopically, and these findings were supported by histopathological lesions in gill and muscle tissues.
format Online
Article
Text
id pubmed-7811552
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher Veterinary World
record_format MEDLINE/PubMed
spelling pubmed-78115522021-01-22 Isolation, molecular identification, and pathological lesions of Saprolegnia spp. isolated from common carp, Cyprinus carpio in floating cages in Mosul, Iraq Ali, Fawwaz Fadhil AL-Taee, Shahbaa Khalil AL-Jumaa, Zahraa Mustafa Vet World Research Article BACKGROUND AND AIM: Saprolegniasis is a fungal disease that infects freshwater fish. The condition is characterized by a cotton-like appearance in the gills and body. This study aimed to isolate Saprolegnia from common carp, Cyprinus carpio, raised in a floating cage in Wana district, Mosul, Iraq. MATERIALS AND METHODS: Samples were collected from 15 infected fish and examined microbiologically, molecularly, and histopathologically. Saprolegnia DNA was extracted which was amplified using universal primers give a 540 bp DNA fragment, and gill and muscle tissue were also examined for histopathological changes. RESULTS: Isolated colonies of Saprolegnia were characterized by a circular, white cottony appearance with long hair. Lactophenol staining demonstrated hyphae as branched non-septate, transparent masses. The genomic DNA of isolates was consistent with Saprolegnia spp. The infected tissue samples showed variable pathology in gills. Severe hemorrhage and edema were observed in primary gill filaments with hyperplasia in epithelial cells and infusion in secondary gill filaments. Hyphae of Saprolegnia were seen between necrotic and edematous myofiber with inflammatory cells infiltration. CONCLUSION: Saprolegnia can cause economic impacts through lethal infection of fish. Clinical signs of Saprolegnia infection were confirmed molecularly and microscopically, and these findings were supported by histopathological lesions in gill and muscle tissues. Veterinary World 2020-12 2020-12-24 /pmc/articles/PMC7811552/ /pubmed/33487995 http://dx.doi.org/10.14202/vetworld.2020.2759-2764 Text en Copyright: © Ali, et al. http://creativecommons.org/licenses/by/4.0 Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Ali, Fawwaz Fadhil
AL-Taee, Shahbaa Khalil
AL-Jumaa, Zahraa Mustafa
Isolation, molecular identification, and pathological lesions of Saprolegnia spp. isolated from common carp, Cyprinus carpio in floating cages in Mosul, Iraq
title Isolation, molecular identification, and pathological lesions of Saprolegnia spp. isolated from common carp, Cyprinus carpio in floating cages in Mosul, Iraq
title_full Isolation, molecular identification, and pathological lesions of Saprolegnia spp. isolated from common carp, Cyprinus carpio in floating cages in Mosul, Iraq
title_fullStr Isolation, molecular identification, and pathological lesions of Saprolegnia spp. isolated from common carp, Cyprinus carpio in floating cages in Mosul, Iraq
title_full_unstemmed Isolation, molecular identification, and pathological lesions of Saprolegnia spp. isolated from common carp, Cyprinus carpio in floating cages in Mosul, Iraq
title_short Isolation, molecular identification, and pathological lesions of Saprolegnia spp. isolated from common carp, Cyprinus carpio in floating cages in Mosul, Iraq
title_sort isolation, molecular identification, and pathological lesions of saprolegnia spp. isolated from common carp, cyprinus carpio in floating cages in mosul, iraq
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7811552/
https://www.ncbi.nlm.nih.gov/pubmed/33487995
http://dx.doi.org/10.14202/vetworld.2020.2759-2764
work_keys_str_mv AT alifawwazfadhil isolationmolecularidentificationandpathologicallesionsofsaprolegniasppisolatedfromcommoncarpcyprinuscarpioinfloatingcagesinmosuliraq
AT altaeeshahbaakhalil isolationmolecularidentificationandpathologicallesionsofsaprolegniasppisolatedfromcommoncarpcyprinuscarpioinfloatingcagesinmosuliraq
AT aljumaazahraamustafa isolationmolecularidentificationandpathologicallesionsofsaprolegniasppisolatedfromcommoncarpcyprinuscarpioinfloatingcagesinmosuliraq