Morphological and intracellular alterations induced by cytotoxin VT2y produced by Escherichia coli isolated from chickens with swollen head syndrome

Recently, a novel verocytotoxin named VT2y was described which belongs to the STx family and is produced by Escherichia coli isolated from domestic poultry with swollen head syndrome (SHS). The VT2y toxin induced apoptosis in Vero, HeLa, CHO, CEF (primary chicken embryo fibroblast) and PCK (primary...

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Autores principales: Salvadori, Marcia R., Yamada, Aureo T., Yano, Tomomasa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Federation of European Microbiological Societies. Published by Elsevier B.V. 2001
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7811797/
http://dx.doi.org/10.1016/S0378-1097(01)00090-8
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author Salvadori, Marcia R.
Yamada, Aureo T.
Yano, Tomomasa
author_facet Salvadori, Marcia R.
Yamada, Aureo T.
Yano, Tomomasa
author_sort Salvadori, Marcia R.
collection PubMed
description Recently, a novel verocytotoxin named VT2y was described which belongs to the STx family and is produced by Escherichia coli isolated from domestic poultry with swollen head syndrome (SHS). The VT2y toxin induced apoptosis in Vero, HeLa, CHO, CEF (primary chicken embryo fibroblast) and PCK (primary chicken kidney) cell lines. Morphological evidence (nuclear shrinkage, chromatin condensation and blebbing of the plasma membrane) of apoptosis could be distinguished in 15 min and was maximal at 1 h after treatment with VT2y. This was confirmed by the terminal dUTP nick-end-labeling (TUNEL) method.
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spelling pubmed-78117972021-01-19 Morphological and intracellular alterations induced by cytotoxin VT2y produced by Escherichia coli isolated from chickens with swollen head syndrome Salvadori, Marcia R. Yamada, Aureo T. Yano, Tomomasa FEMS Microbiol Lett Article Recently, a novel verocytotoxin named VT2y was described which belongs to the STx family and is produced by Escherichia coli isolated from domestic poultry with swollen head syndrome (SHS). The VT2y toxin induced apoptosis in Vero, HeLa, CHO, CEF (primary chicken embryo fibroblast) and PCK (primary chicken kidney) cell lines. Morphological evidence (nuclear shrinkage, chromatin condensation and blebbing of the plasma membrane) of apoptosis could be distinguished in 15 min and was maximal at 1 h after treatment with VT2y. This was confirmed by the terminal dUTP nick-end-labeling (TUNEL) method. Federation of European Microbiological Societies. Published by Elsevier B.V. 2001-04-01 2001-03-28 /pmc/articles/PMC7811797/ http://dx.doi.org/10.1016/S0378-1097(01)00090-8 Text en Copyright © 2001 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Salvadori, Marcia R.
Yamada, Aureo T.
Yano, Tomomasa
Morphological and intracellular alterations induced by cytotoxin VT2y produced by Escherichia coli isolated from chickens with swollen head syndrome
title Morphological and intracellular alterations induced by cytotoxin VT2y produced by Escherichia coli isolated from chickens with swollen head syndrome
title_full Morphological and intracellular alterations induced by cytotoxin VT2y produced by Escherichia coli isolated from chickens with swollen head syndrome
title_fullStr Morphological and intracellular alterations induced by cytotoxin VT2y produced by Escherichia coli isolated from chickens with swollen head syndrome
title_full_unstemmed Morphological and intracellular alterations induced by cytotoxin VT2y produced by Escherichia coli isolated from chickens with swollen head syndrome
title_short Morphological and intracellular alterations induced by cytotoxin VT2y produced by Escherichia coli isolated from chickens with swollen head syndrome
title_sort morphological and intracellular alterations induced by cytotoxin vt2y produced by escherichia coli isolated from chickens with swollen head syndrome
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7811797/
http://dx.doi.org/10.1016/S0378-1097(01)00090-8
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