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Lung organoids: advances in generation and 3D-visualization

The lung is comprised of more than 40 distinct cell types that support a complex 3-dimensional (3D) architecture that is required for efficient lung function. Loss of this proper architecture can accommodate and promote lung disease, highlighting researchers’ growing need to analyze lung structures...

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Autores principales: Cunniff, Brian, Druso, Joseph E., van der Velden, Jos L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7812034/
https://www.ncbi.nlm.nih.gov/pubmed/33459870
http://dx.doi.org/10.1007/s00418-020-01955-w
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author Cunniff, Brian
Druso, Joseph E.
van der Velden, Jos L.
author_facet Cunniff, Brian
Druso, Joseph E.
van der Velden, Jos L.
author_sort Cunniff, Brian
collection PubMed
description The lung is comprised of more than 40 distinct cell types that support a complex 3-dimensional (3D) architecture that is required for efficient lung function. Loss of this proper architecture can accommodate and promote lung disease, highlighting researchers’ growing need to analyze lung structures in detail. Additionally, in vivo cellular and molecular response to chemical and physical signals, along with the recapitulation of gene-expression patterns, can be lost during the transition from complex 3D tissues to 2D cell culture systems. Therefore, technologies that allow for the investigation of lung function under normal and disease states utilizing the entirety of the lung architecture are required to generate a complete understanding of these processes. Airway cell-derived organoids that can recapitulate lung structure and function ex vivo while being amenable to experimental manipulation, have provided a new and exciting model system to investigate lung biology. In this perspective, we discuss emerging technologies for culturing lung-derived organoids, techniques to visualize organoids using high-resolution microscopy and the resulting information extracted from organoids supporting research focused on lung function and diseases. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s00418-020-01955-w.
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spelling pubmed-78120342021-01-18 Lung organoids: advances in generation and 3D-visualization Cunniff, Brian Druso, Joseph E. van der Velden, Jos L. Histochem Cell Biol Review The lung is comprised of more than 40 distinct cell types that support a complex 3-dimensional (3D) architecture that is required for efficient lung function. Loss of this proper architecture can accommodate and promote lung disease, highlighting researchers’ growing need to analyze lung structures in detail. Additionally, in vivo cellular and molecular response to chemical and physical signals, along with the recapitulation of gene-expression patterns, can be lost during the transition from complex 3D tissues to 2D cell culture systems. Therefore, technologies that allow for the investigation of lung function under normal and disease states utilizing the entirety of the lung architecture are required to generate a complete understanding of these processes. Airway cell-derived organoids that can recapitulate lung structure and function ex vivo while being amenable to experimental manipulation, have provided a new and exciting model system to investigate lung biology. In this perspective, we discuss emerging technologies for culturing lung-derived organoids, techniques to visualize organoids using high-resolution microscopy and the resulting information extracted from organoids supporting research focused on lung function and diseases. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s00418-020-01955-w. Springer Berlin Heidelberg 2021-01-18 2021 /pmc/articles/PMC7812034/ /pubmed/33459870 http://dx.doi.org/10.1007/s00418-020-01955-w Text en © The Author(s) 2021 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Review
Cunniff, Brian
Druso, Joseph E.
van der Velden, Jos L.
Lung organoids: advances in generation and 3D-visualization
title Lung organoids: advances in generation and 3D-visualization
title_full Lung organoids: advances in generation and 3D-visualization
title_fullStr Lung organoids: advances in generation and 3D-visualization
title_full_unstemmed Lung organoids: advances in generation and 3D-visualization
title_short Lung organoids: advances in generation and 3D-visualization
title_sort lung organoids: advances in generation and 3d-visualization
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7812034/
https://www.ncbi.nlm.nih.gov/pubmed/33459870
http://dx.doi.org/10.1007/s00418-020-01955-w
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