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Serum proteomic analysis of novel predictive serum proteins for neurological prognosis following cardiac arrest

Early prognostication of neurological outcome in comatose patients after cardiac arrest (CA) is vital for clinicians when assessing the survival time of sufferers and formulating appropriate treatment strategies to avoid the withdrawal of life‐sustaining treatment (WLST) from patients. However, ther...

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Autores principales: Gu, Shuang‐shuang, Li, Jin, Jiang, Min, Zhou, Yi, Yang, Bing, Xie, Kehui, Jiang, Yun‐fei, Jiang, Xin‐rui, He, Fei, Wang, Jun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7812277/
https://www.ncbi.nlm.nih.gov/pubmed/33336526
http://dx.doi.org/10.1111/jcmm.16201
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author Gu, Shuang‐shuang
Li, Jin
Jiang, Min
Zhou, Yi
Yang, Bing
Xie, Kehui
Jiang, Yun‐fei
Jiang, Xin‐rui
He, Fei
Wang, Jun
author_facet Gu, Shuang‐shuang
Li, Jin
Jiang, Min
Zhou, Yi
Yang, Bing
Xie, Kehui
Jiang, Yun‐fei
Jiang, Xin‐rui
He, Fei
Wang, Jun
author_sort Gu, Shuang‐shuang
collection PubMed
description Early prognostication of neurological outcome in comatose patients after cardiac arrest (CA) is vital for clinicians when assessing the survival time of sufferers and formulating appropriate treatment strategies to avoid the withdrawal of life‐sustaining treatment (WLST) from patients. However, there is still a lack of sensitive and specific serum biomarkers for early and accurate identification of these patients. Using an isobaric tag for relative and absolute quantitation (iTRAQ)‐based proteomic approach, we discovered 55 differentially expressed proteins, with 39 up‐regulated secreted serum proteins and 16 down‐regulated secreted serum proteins between three comatose CA survivors with good versus poor neurological recovery. Then, four proteins were selected and were validated via an enzyme‐linked immunosorbent assay (ELISA) approach in a larger‐scale sample containing 32 good neurological outcome patients and 46 poor neurological outcome patients, and it was confirmed that serum angiotensinogen (AGT) and alpha‐1‐antitrypsin (SERPINA1) were associated with neurological function and prognosis in CA survivors. A prognostic risk score was developed and calculated using a linear and logistic regression model based on a combination of AGT, SERPINA1 and neuron‐specific enolase (NSE) with an area under the curve of 0.865 (P < .001), and the prognostic risk score was positively correlated with the CPC value (R = 0.708, P < .001). We propose that the results of the risk score assessment not only reveal changes in biomarkers during neurological recovery but also assist in enhancing current therapeutic strategies for comatose CA survivors.
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spelling pubmed-78122772021-01-22 Serum proteomic analysis of novel predictive serum proteins for neurological prognosis following cardiac arrest Gu, Shuang‐shuang Li, Jin Jiang, Min Zhou, Yi Yang, Bing Xie, Kehui Jiang, Yun‐fei Jiang, Xin‐rui He, Fei Wang, Jun J Cell Mol Med Original Articles Early prognostication of neurological outcome in comatose patients after cardiac arrest (CA) is vital for clinicians when assessing the survival time of sufferers and formulating appropriate treatment strategies to avoid the withdrawal of life‐sustaining treatment (WLST) from patients. However, there is still a lack of sensitive and specific serum biomarkers for early and accurate identification of these patients. Using an isobaric tag for relative and absolute quantitation (iTRAQ)‐based proteomic approach, we discovered 55 differentially expressed proteins, with 39 up‐regulated secreted serum proteins and 16 down‐regulated secreted serum proteins between three comatose CA survivors with good versus poor neurological recovery. Then, four proteins were selected and were validated via an enzyme‐linked immunosorbent assay (ELISA) approach in a larger‐scale sample containing 32 good neurological outcome patients and 46 poor neurological outcome patients, and it was confirmed that serum angiotensinogen (AGT) and alpha‐1‐antitrypsin (SERPINA1) were associated with neurological function and prognosis in CA survivors. A prognostic risk score was developed and calculated using a linear and logistic regression model based on a combination of AGT, SERPINA1 and neuron‐specific enolase (NSE) with an area under the curve of 0.865 (P < .001), and the prognostic risk score was positively correlated with the CPC value (R = 0.708, P < .001). We propose that the results of the risk score assessment not only reveal changes in biomarkers during neurological recovery but also assist in enhancing current therapeutic strategies for comatose CA survivors. John Wiley and Sons Inc. 2020-12-18 2021-01 /pmc/articles/PMC7812277/ /pubmed/33336526 http://dx.doi.org/10.1111/jcmm.16201 Text en © 2020 The Authors. Journal of Cellular and Molecular Medicine published by Foundation for Cellular and Molecular Medicine and John Wiley & Sons Ltd. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Articles
Gu, Shuang‐shuang
Li, Jin
Jiang, Min
Zhou, Yi
Yang, Bing
Xie, Kehui
Jiang, Yun‐fei
Jiang, Xin‐rui
He, Fei
Wang, Jun
Serum proteomic analysis of novel predictive serum proteins for neurological prognosis following cardiac arrest
title Serum proteomic analysis of novel predictive serum proteins for neurological prognosis following cardiac arrest
title_full Serum proteomic analysis of novel predictive serum proteins for neurological prognosis following cardiac arrest
title_fullStr Serum proteomic analysis of novel predictive serum proteins for neurological prognosis following cardiac arrest
title_full_unstemmed Serum proteomic analysis of novel predictive serum proteins for neurological prognosis following cardiac arrest
title_short Serum proteomic analysis of novel predictive serum proteins for neurological prognosis following cardiac arrest
title_sort serum proteomic analysis of novel predictive serum proteins for neurological prognosis following cardiac arrest
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7812277/
https://www.ncbi.nlm.nih.gov/pubmed/33336526
http://dx.doi.org/10.1111/jcmm.16201
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