Comparison of N- and C-terminally endogenously GFP-tagged WEE-1.3 strains in C. elegans

We have generated a WEE-1.3 strain in C. elegans wherewe have endogenously tagged the C-terminus with GFP. In this publication we demonstrate that this new strain exhibits the same expression localization pattern as the WEE-1.3 antibody and N-terminally endogenously GFP-tagged WEE-1.3 strain that ha...

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Detalles Bibliográficos
Autores principales: Fernando, Lourds M., Golliday, Kyrionna, Boateng, Ruby, Allen, Anna K.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Caltech Library 2021
Materias:
New Finding
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7812379/
https://www.ncbi.nlm.nih.gov/pubmed/33474525
http://dx.doi.org/10.17912/micropub.biology.000353
Descripción
Sumario:We have generated a WEE-1.3 strain in C. elegans wherewe have endogenously tagged the C-terminus with GFP. In this publication we demonstrate that this new strain exhibits the same expression localization pattern as the WEE-1.3 antibody and N-terminally endogenously GFP-tagged WEE-1.3 strain that have been previously published. We also show for the first time that endogenously tagging WEE-1.3 at either termini does not affect the reproductive function of the worms.

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