Comparison of N- and C-terminally endogenously GFP-tagged WEE-1.3 strains in C. elegans

We have generated a WEE-1.3 strain in C. elegans wherewe have endogenously tagged the C-terminus with GFP. In this publication we demonstrate that this new strain exhibits the same expression localization pattern as the WEE-1.3 antibody and N-terminally endogenously GFP-tagged WEE-1.3 strain that ha...

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Detalles Bibliográficos
Autores principales: Fernando, Lourds M., Golliday, Kyrionna, Boateng, Ruby, Allen, Anna K.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Caltech Library 2021
Materias:
New Finding
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7812379/
https://www.ncbi.nlm.nih.gov/pubmed/33474525
http://dx.doi.org/10.17912/micropub.biology.000353
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author Fernando, Lourds M.
Golliday, Kyrionna
Boateng, Ruby
Allen, Anna K.
author_facet Fernando, Lourds M.
Golliday, Kyrionna
Boateng, Ruby
Allen, Anna K.
author_sort Fernando, Lourds M.
collection PubMed
description We have generated a WEE-1.3 strain in C. elegans wherewe have endogenously tagged the C-terminus with GFP. In this publication we demonstrate that this new strain exhibits the same expression localization pattern as the WEE-1.3 antibody and N-terminally endogenously GFP-tagged WEE-1.3 strain that have been previously published. We also show for the first time that endogenously tagging WEE-1.3 at either termini does not affect the reproductive function of the worms.
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spelling pubmed-78123792021-01-19 Comparison of N- and C-terminally endogenously GFP-tagged WEE-1.3 strains in C. elegans Fernando, Lourds M. Golliday, Kyrionna Boateng, Ruby Allen, Anna K. MicroPubl Biol New Finding We have generated a WEE-1.3 strain in C. elegans wherewe have endogenously tagged the C-terminus with GFP. In this publication we demonstrate that this new strain exhibits the same expression localization pattern as the WEE-1.3 antibody and N-terminally endogenously GFP-tagged WEE-1.3 strain that have been previously published. We also show for the first time that endogenously tagging WEE-1.3 at either termini does not affect the reproductive function of the worms. Caltech Library 2021-01-14 /pmc/articles/PMC7812379/ /pubmed/33474525 http://dx.doi.org/10.17912/micropub.biology.000353 Text en Copyright: © 2021 by the authors https://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle New Finding
Fernando, Lourds M.
Golliday, Kyrionna
Boateng, Ruby
Allen, Anna K.
Comparison of N- and C-terminally endogenously GFP-tagged WEE-1.3 strains in C. elegans
title Comparison of N- and C-terminally endogenously GFP-tagged WEE-1.3 strains in C. elegans
title_full Comparison of N- and C-terminally endogenously GFP-tagged WEE-1.3 strains in C. elegans
title_fullStr Comparison of N- and C-terminally endogenously GFP-tagged WEE-1.3 strains in C. elegans
title_full_unstemmed Comparison of N- and C-terminally endogenously GFP-tagged WEE-1.3 strains in C. elegans
title_short Comparison of N- and C-terminally endogenously GFP-tagged WEE-1.3 strains in C. elegans
title_sort comparison of n- and c-terminally endogenously gfp-tagged wee-1.3 strains in c. elegans
topic New Finding
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7812379/
https://www.ncbi.nlm.nih.gov/pubmed/33474525
http://dx.doi.org/10.17912/micropub.biology.000353
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