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Effects of estradiol on in vitro maturation of buffalo and goat oocytes

PURPOSE: The effects of estradiol on oocyte development seem to be varied among species. The present study investigated the effects of 17β‐estradiol on in vitro maturation of buffalo and goat oocytes. METHODS: Cumulus oocyte complexes (COCs) were aspirated from large antral follicles of slaughtered...

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Detalles Bibliográficos
Autores principales: Maksura, Hurum, Akon, Narsisa, Islam, Md Nuronnabi, Akter, Ireen, Modak, Avijit Kumar, Khatun, Asma, Alam, Md Hasanur, Hashem, Md Abul, Amin, Md Ruhul, Moniruzzaman, Mohammad
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7812509/
https://www.ncbi.nlm.nih.gov/pubmed/33488284
http://dx.doi.org/10.1002/rmb2.12350
Descripción
Sumario:PURPOSE: The effects of estradiol on oocyte development seem to be varied among species. The present study investigated the effects of 17β‐estradiol on in vitro maturation of buffalo and goat oocytes. METHODS: Cumulus oocyte complexes (COCs) were aspirated from large antral follicles of slaughtered buffalo and goat ovaries. COCs were cultured in TCM‐199 medium supplemented with 0, 0.5, 1, and 1.5 µg/mL of 17β‐estradiol for in vitro maturation. Then, oocytes were used for the examination of state of nuclear maturation and cumulus expansion. RESULTS: In both species, oocytes treated with 17β‐estradiol showed higher cumulus expansion rate than control (0 µg/mL treated). In buffalo, the percentage of oocytes matured to the metaphase II (MII) stage increased in the concentration‐dependent manner of 17β‐estradiol. Similarly, estradiol positively influenced nuclear maturation of goat oocytes in vitro. CONCLUSIONS: Estradiol has promoting effects on normalprogress of in vitro oocyte meiosis in buffalos and goats.