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Effects of estradiol on in vitro maturation of buffalo and goat oocytes
PURPOSE: The effects of estradiol on oocyte development seem to be varied among species. The present study investigated the effects of 17β‐estradiol on in vitro maturation of buffalo and goat oocytes. METHODS: Cumulus oocyte complexes (COCs) were aspirated from large antral follicles of slaughtered...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7812509/ https://www.ncbi.nlm.nih.gov/pubmed/33488284 http://dx.doi.org/10.1002/rmb2.12350 |
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author | Maksura, Hurum Akon, Narsisa Islam, Md Nuronnabi Akter, Ireen Modak, Avijit Kumar Khatun, Asma Alam, Md Hasanur Hashem, Md Abul Amin, Md Ruhul Moniruzzaman, Mohammad |
author_facet | Maksura, Hurum Akon, Narsisa Islam, Md Nuronnabi Akter, Ireen Modak, Avijit Kumar Khatun, Asma Alam, Md Hasanur Hashem, Md Abul Amin, Md Ruhul Moniruzzaman, Mohammad |
author_sort | Maksura, Hurum |
collection | PubMed |
description | PURPOSE: The effects of estradiol on oocyte development seem to be varied among species. The present study investigated the effects of 17β‐estradiol on in vitro maturation of buffalo and goat oocytes. METHODS: Cumulus oocyte complexes (COCs) were aspirated from large antral follicles of slaughtered buffalo and goat ovaries. COCs were cultured in TCM‐199 medium supplemented with 0, 0.5, 1, and 1.5 µg/mL of 17β‐estradiol for in vitro maturation. Then, oocytes were used for the examination of state of nuclear maturation and cumulus expansion. RESULTS: In both species, oocytes treated with 17β‐estradiol showed higher cumulus expansion rate than control (0 µg/mL treated). In buffalo, the percentage of oocytes matured to the metaphase II (MII) stage increased in the concentration‐dependent manner of 17β‐estradiol. Similarly, estradiol positively influenced nuclear maturation of goat oocytes in vitro. CONCLUSIONS: Estradiol has promoting effects on normalprogress of in vitro oocyte meiosis in buffalos and goats. |
format | Online Article Text |
id | pubmed-7812509 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-78125092021-01-22 Effects of estradiol on in vitro maturation of buffalo and goat oocytes Maksura, Hurum Akon, Narsisa Islam, Md Nuronnabi Akter, Ireen Modak, Avijit Kumar Khatun, Asma Alam, Md Hasanur Hashem, Md Abul Amin, Md Ruhul Moniruzzaman, Mohammad Reprod Med Biol Original Articles PURPOSE: The effects of estradiol on oocyte development seem to be varied among species. The present study investigated the effects of 17β‐estradiol on in vitro maturation of buffalo and goat oocytes. METHODS: Cumulus oocyte complexes (COCs) were aspirated from large antral follicles of slaughtered buffalo and goat ovaries. COCs were cultured in TCM‐199 medium supplemented with 0, 0.5, 1, and 1.5 µg/mL of 17β‐estradiol for in vitro maturation. Then, oocytes were used for the examination of state of nuclear maturation and cumulus expansion. RESULTS: In both species, oocytes treated with 17β‐estradiol showed higher cumulus expansion rate than control (0 µg/mL treated). In buffalo, the percentage of oocytes matured to the metaphase II (MII) stage increased in the concentration‐dependent manner of 17β‐estradiol. Similarly, estradiol positively influenced nuclear maturation of goat oocytes in vitro. CONCLUSIONS: Estradiol has promoting effects on normalprogress of in vitro oocyte meiosis in buffalos and goats. John Wiley and Sons Inc. 2020-09-25 /pmc/articles/PMC7812509/ /pubmed/33488284 http://dx.doi.org/10.1002/rmb2.12350 Text en © 2020 The Authors. Reproductive Medicine and Biology published by John Wiley & Sons Australia, Ltd on behalf of Japan Society for Reproductive Medicine This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Articles Maksura, Hurum Akon, Narsisa Islam, Md Nuronnabi Akter, Ireen Modak, Avijit Kumar Khatun, Asma Alam, Md Hasanur Hashem, Md Abul Amin, Md Ruhul Moniruzzaman, Mohammad Effects of estradiol on in vitro maturation of buffalo and goat oocytes |
title | Effects of estradiol on in vitro maturation of buffalo and goat oocytes |
title_full | Effects of estradiol on in vitro maturation of buffalo and goat oocytes |
title_fullStr | Effects of estradiol on in vitro maturation of buffalo and goat oocytes |
title_full_unstemmed | Effects of estradiol on in vitro maturation of buffalo and goat oocytes |
title_short | Effects of estradiol on in vitro maturation of buffalo and goat oocytes |
title_sort | effects of estradiol on in vitro maturation of buffalo and goat oocytes |
topic | Original Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7812509/ https://www.ncbi.nlm.nih.gov/pubmed/33488284 http://dx.doi.org/10.1002/rmb2.12350 |
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