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SNORD63 and SNORD96A as the non-invasive diagnostic biomarkers for clear cell renal cell carcinoma
BACKGROUND: Increasing evidence has demonstrated that snoRNAs play crucial roles in tumorigenesis of various cancer types. However, researches on snoRNAs in ccRCC were very little. This study mainly aimed to validate the differential expression and the potential diagnostic value of SNORD63 and SNORD...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7812721/ https://www.ncbi.nlm.nih.gov/pubmed/33461545 http://dx.doi.org/10.1186/s12935-020-01744-4 |
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author | Shang, Xiaoling Song, Xingguo Wang, Kangyu Yu, Miao Ding, Shanshan Dong, Xiaohan Xie, Li Song, Xianrang |
author_facet | Shang, Xiaoling Song, Xingguo Wang, Kangyu Yu, Miao Ding, Shanshan Dong, Xiaohan Xie, Li Song, Xianrang |
author_sort | Shang, Xiaoling |
collection | PubMed |
description | BACKGROUND: Increasing evidence has demonstrated that snoRNAs play crucial roles in tumorigenesis of various cancer types. However, researches on snoRNAs in ccRCC were very little. This study mainly aimed to validate the differential expression and the potential diagnostic value of SNORD63 and SNORD96A in ccRCC. METHODS: SnoRNAs expression was downloaded from the SNORic and TCGA database including 516 patients with ccRCC and 71 control cases. SNORD63 and SNORD96A expression were further detected in 54 tumor and adjacent FFPE ccRCC tissues, 55 plasma and 75 urinary sediment of ccRCC patients. Then, differential expression and diagnostic value of SNORD63 and SNORD96A were further calculated. RESULTS: SNORD63 and SNORD96A expression were significantly increased in ccRCC tissues compared with normal tissues from the TCGA database (both, P < 0.0001). In addition, we found that SNORD63 and SNORD96A localized in plasma and US stably after treating with RNase A. Meanwhile, SNORD63 and SNORD96A in FFPE and US were elevated in ccRCC patients (all, P < 0.0001). However, plasma SNORD63 expression had no significance while SNORD96A significantly increased in plasma of ccRCC patients. Notably, the AUC of SNORD63 in US was 0.7055, by comparison the AUC of plasma SNORD63 was only 0.5161. However, the AUC of plasma SNORD96A was up to 0.8909, by comparison the AUC of SNORD96A in US was 0.6788. Interestingly, the AUC of plasma SNORD96A in early stage ccRCC was highly up to 0.9359. CONCLUSIONS: Our findings revealed that SNORD63 in US and SNORD96A in plasma could act as the promising non-invasive diagnostic biomarkers for ccRCC patients. |
format | Online Article Text |
id | pubmed-7812721 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-78127212021-01-19 SNORD63 and SNORD96A as the non-invasive diagnostic biomarkers for clear cell renal cell carcinoma Shang, Xiaoling Song, Xingguo Wang, Kangyu Yu, Miao Ding, Shanshan Dong, Xiaohan Xie, Li Song, Xianrang Cancer Cell Int Primary Research BACKGROUND: Increasing evidence has demonstrated that snoRNAs play crucial roles in tumorigenesis of various cancer types. However, researches on snoRNAs in ccRCC were very little. This study mainly aimed to validate the differential expression and the potential diagnostic value of SNORD63 and SNORD96A in ccRCC. METHODS: SnoRNAs expression was downloaded from the SNORic and TCGA database including 516 patients with ccRCC and 71 control cases. SNORD63 and SNORD96A expression were further detected in 54 tumor and adjacent FFPE ccRCC tissues, 55 plasma and 75 urinary sediment of ccRCC patients. Then, differential expression and diagnostic value of SNORD63 and SNORD96A were further calculated. RESULTS: SNORD63 and SNORD96A expression were significantly increased in ccRCC tissues compared with normal tissues from the TCGA database (both, P < 0.0001). In addition, we found that SNORD63 and SNORD96A localized in plasma and US stably after treating with RNase A. Meanwhile, SNORD63 and SNORD96A in FFPE and US were elevated in ccRCC patients (all, P < 0.0001). However, plasma SNORD63 expression had no significance while SNORD96A significantly increased in plasma of ccRCC patients. Notably, the AUC of SNORD63 in US was 0.7055, by comparison the AUC of plasma SNORD63 was only 0.5161. However, the AUC of plasma SNORD96A was up to 0.8909, by comparison the AUC of SNORD96A in US was 0.6788. Interestingly, the AUC of plasma SNORD96A in early stage ccRCC was highly up to 0.9359. CONCLUSIONS: Our findings revealed that SNORD63 in US and SNORD96A in plasma could act as the promising non-invasive diagnostic biomarkers for ccRCC patients. BioMed Central 2021-01-18 /pmc/articles/PMC7812721/ /pubmed/33461545 http://dx.doi.org/10.1186/s12935-020-01744-4 Text en © The Author(s) 2021 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Primary Research Shang, Xiaoling Song, Xingguo Wang, Kangyu Yu, Miao Ding, Shanshan Dong, Xiaohan Xie, Li Song, Xianrang SNORD63 and SNORD96A as the non-invasive diagnostic biomarkers for clear cell renal cell carcinoma |
title | SNORD63 and SNORD96A as the non-invasive diagnostic biomarkers for clear cell renal cell carcinoma |
title_full | SNORD63 and SNORD96A as the non-invasive diagnostic biomarkers for clear cell renal cell carcinoma |
title_fullStr | SNORD63 and SNORD96A as the non-invasive diagnostic biomarkers for clear cell renal cell carcinoma |
title_full_unstemmed | SNORD63 and SNORD96A as the non-invasive diagnostic biomarkers for clear cell renal cell carcinoma |
title_short | SNORD63 and SNORD96A as the non-invasive diagnostic biomarkers for clear cell renal cell carcinoma |
title_sort | snord63 and snord96a as the non-invasive diagnostic biomarkers for clear cell renal cell carcinoma |
topic | Primary Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7812721/ https://www.ncbi.nlm.nih.gov/pubmed/33461545 http://dx.doi.org/10.1186/s12935-020-01744-4 |
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