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Inhibition of Circulating Exosomal miRNA-20b-5p Accelerates Diabetic Wound Repair

PURPOSE: Efficient approaches to reliably improving wound healing in diabetic patients remain to be developed. Exosomes are nanomaterials from which therapeutically active microRNAs (miRNAs) can be isolated. In the present report, we therefore isolated circulating exosome-derived miRNAs from patient...

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Autores principales: Chen, Kai, Yu, Tao, Wang, Xin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7813471/
https://www.ncbi.nlm.nih.gov/pubmed/33469291
http://dx.doi.org/10.2147/IJN.S287875
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author Chen, Kai
Yu, Tao
Wang, Xin
author_facet Chen, Kai
Yu, Tao
Wang, Xin
author_sort Chen, Kai
collection PubMed
description PURPOSE: Efficient approaches to reliably improving wound healing in diabetic patients remain to be developed. Exosomes are nanomaterials from which therapeutically active microRNAs (miRNAs) can be isolated. In the present report, we therefore isolated circulating exosome-derived miRNAs from patients with diabetes and assessed the impact of these molecules on wound healing. PATIENTS AND METHODS: Exosomes were isolated from the serum of control or diabetic patients (Con-Exos and Dia-Exos, respectively), after which the effects of these exosomes on cellular activity and wound healing were assessed. RESULTS: We determined that miR-20b-5p was overexpressed in Dia-Exos and that it functioned by impairing wound repair by suppressing vascular endothelial growth factor A (VEGFA) expression. Consistent with such a model, the administration of Dia-Exos or this miRNA both in vivo and in vitro was sufficient to slow wound repair. CONCLUSION: Dia-Exos exhibit significant increases in miR-20b-5p relative to Con-Exos, and this miRNA can be transferred into HSFs wherein it can suppress VEGFA expression and thereby slow the process of wound healing.
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spelling pubmed-78134712021-01-18 Inhibition of Circulating Exosomal miRNA-20b-5p Accelerates Diabetic Wound Repair Chen, Kai Yu, Tao Wang, Xin Int J Nanomedicine Original Research PURPOSE: Efficient approaches to reliably improving wound healing in diabetic patients remain to be developed. Exosomes are nanomaterials from which therapeutically active microRNAs (miRNAs) can be isolated. In the present report, we therefore isolated circulating exosome-derived miRNAs from patients with diabetes and assessed the impact of these molecules on wound healing. PATIENTS AND METHODS: Exosomes were isolated from the serum of control or diabetic patients (Con-Exos and Dia-Exos, respectively), after which the effects of these exosomes on cellular activity and wound healing were assessed. RESULTS: We determined that miR-20b-5p was overexpressed in Dia-Exos and that it functioned by impairing wound repair by suppressing vascular endothelial growth factor A (VEGFA) expression. Consistent with such a model, the administration of Dia-Exos or this miRNA both in vivo and in vitro was sufficient to slow wound repair. CONCLUSION: Dia-Exos exhibit significant increases in miR-20b-5p relative to Con-Exos, and this miRNA can be transferred into HSFs wherein it can suppress VEGFA expression and thereby slow the process of wound healing. Dove 2021-01-14 /pmc/articles/PMC7813471/ /pubmed/33469291 http://dx.doi.org/10.2147/IJN.S287875 Text en © 2021 Chen et al. http://creativecommons.org/licenses/by-nc/3.0/ This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php).
spellingShingle Original Research
Chen, Kai
Yu, Tao
Wang, Xin
Inhibition of Circulating Exosomal miRNA-20b-5p Accelerates Diabetic Wound Repair
title Inhibition of Circulating Exosomal miRNA-20b-5p Accelerates Diabetic Wound Repair
title_full Inhibition of Circulating Exosomal miRNA-20b-5p Accelerates Diabetic Wound Repair
title_fullStr Inhibition of Circulating Exosomal miRNA-20b-5p Accelerates Diabetic Wound Repair
title_full_unstemmed Inhibition of Circulating Exosomal miRNA-20b-5p Accelerates Diabetic Wound Repair
title_short Inhibition of Circulating Exosomal miRNA-20b-5p Accelerates Diabetic Wound Repair
title_sort inhibition of circulating exosomal mirna-20b-5p accelerates diabetic wound repair
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7813471/
https://www.ncbi.nlm.nih.gov/pubmed/33469291
http://dx.doi.org/10.2147/IJN.S287875
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