Cargando…

A rapid diagnosis of SARS-CoV-2 using DNA hydrogel formation on microfluidic pores

The coronavirus disease 2019 (COVID-19) pandemic has been a major public health challenge in 2020. Early diagnosis of COVID-19 is the most effective method to control disease spread and prevent further mortality. As such, a high-precision and rapid yet economic assay method is urgently required. Her...

Descripción completa

Detalles Bibliográficos
Autores principales: Kim, Hwang-soo, Abbas, Naseem, Shin, Sehyun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Authors. Published by Elsevier B.V. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7813512/
https://www.ncbi.nlm.nih.gov/pubmed/33486135
http://dx.doi.org/10.1016/j.bios.2021.113005
_version_ 1783637864715649024
author Kim, Hwang-soo
Abbas, Naseem
Shin, Sehyun
author_facet Kim, Hwang-soo
Abbas, Naseem
Shin, Sehyun
author_sort Kim, Hwang-soo
collection PubMed
description The coronavirus disease 2019 (COVID-19) pandemic has been a major public health challenge in 2020. Early diagnosis of COVID-19 is the most effective method to control disease spread and prevent further mortality. As such, a high-precision and rapid yet economic assay method is urgently required. Herein, we propose an innovative method to detect severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) using isothermal amplification of nucleic acids on a mesh containing multiple microfluidic pores. Hybridization of pathogen DNA and immobilized probes forms a DNA hydrogel by rolling circle amplification and, consequently, blocks the pores to prevent fluid movement, as observed. Following optimization of several factors, including pore size, mesh location, and precision microfluidics, the limit of detection (LOD) for SARS-CoV-2 was determined to be 0.7 aM at 15-min incubation. These results indicate rapid, easy, and effective detection with a moderate-sized LOD of the target pathogen by remote point-of-care testing and without the requirement of any sophisticated device.
format Online
Article
Text
id pubmed-7813512
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher The Authors. Published by Elsevier B.V.
record_format MEDLINE/PubMed
spelling pubmed-78135122021-01-19 A rapid diagnosis of SARS-CoV-2 using DNA hydrogel formation on microfluidic pores Kim, Hwang-soo Abbas, Naseem Shin, Sehyun Biosens Bioelectron Article The coronavirus disease 2019 (COVID-19) pandemic has been a major public health challenge in 2020. Early diagnosis of COVID-19 is the most effective method to control disease spread and prevent further mortality. As such, a high-precision and rapid yet economic assay method is urgently required. Herein, we propose an innovative method to detect severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) using isothermal amplification of nucleic acids on a mesh containing multiple microfluidic pores. Hybridization of pathogen DNA and immobilized probes forms a DNA hydrogel by rolling circle amplification and, consequently, blocks the pores to prevent fluid movement, as observed. Following optimization of several factors, including pore size, mesh location, and precision microfluidics, the limit of detection (LOD) for SARS-CoV-2 was determined to be 0.7 aM at 15-min incubation. These results indicate rapid, easy, and effective detection with a moderate-sized LOD of the target pathogen by remote point-of-care testing and without the requirement of any sophisticated device. The Authors. Published by Elsevier B.V. 2021-04-01 2021-01-18 /pmc/articles/PMC7813512/ /pubmed/33486135 http://dx.doi.org/10.1016/j.bios.2021.113005 Text en © 2021 The Authors Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Kim, Hwang-soo
Abbas, Naseem
Shin, Sehyun
A rapid diagnosis of SARS-CoV-2 using DNA hydrogel formation on microfluidic pores
title A rapid diagnosis of SARS-CoV-2 using DNA hydrogel formation on microfluidic pores
title_full A rapid diagnosis of SARS-CoV-2 using DNA hydrogel formation on microfluidic pores
title_fullStr A rapid diagnosis of SARS-CoV-2 using DNA hydrogel formation on microfluidic pores
title_full_unstemmed A rapid diagnosis of SARS-CoV-2 using DNA hydrogel formation on microfluidic pores
title_short A rapid diagnosis of SARS-CoV-2 using DNA hydrogel formation on microfluidic pores
title_sort rapid diagnosis of sars-cov-2 using dna hydrogel formation on microfluidic pores
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7813512/
https://www.ncbi.nlm.nih.gov/pubmed/33486135
http://dx.doi.org/10.1016/j.bios.2021.113005
work_keys_str_mv AT kimhwangsoo arapiddiagnosisofsarscov2usingdnahydrogelformationonmicrofluidicpores
AT abbasnaseem arapiddiagnosisofsarscov2usingdnahydrogelformationonmicrofluidicpores
AT shinsehyun arapiddiagnosisofsarscov2usingdnahydrogelformationonmicrofluidicpores
AT kimhwangsoo rapiddiagnosisofsarscov2usingdnahydrogelformationonmicrofluidicpores
AT abbasnaseem rapiddiagnosisofsarscov2usingdnahydrogelformationonmicrofluidicpores
AT shinsehyun rapiddiagnosisofsarscov2usingdnahydrogelformationonmicrofluidicpores