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A rapid diagnosis of SARS-CoV-2 using DNA hydrogel formation on microfluidic pores
The coronavirus disease 2019 (COVID-19) pandemic has been a major public health challenge in 2020. Early diagnosis of COVID-19 is the most effective method to control disease spread and prevent further mortality. As such, a high-precision and rapid yet economic assay method is urgently required. Her...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Authors. Published by Elsevier B.V.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7813512/ https://www.ncbi.nlm.nih.gov/pubmed/33486135 http://dx.doi.org/10.1016/j.bios.2021.113005 |
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author | Kim, Hwang-soo Abbas, Naseem Shin, Sehyun |
author_facet | Kim, Hwang-soo Abbas, Naseem Shin, Sehyun |
author_sort | Kim, Hwang-soo |
collection | PubMed |
description | The coronavirus disease 2019 (COVID-19) pandemic has been a major public health challenge in 2020. Early diagnosis of COVID-19 is the most effective method to control disease spread and prevent further mortality. As such, a high-precision and rapid yet economic assay method is urgently required. Herein, we propose an innovative method to detect severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) using isothermal amplification of nucleic acids on a mesh containing multiple microfluidic pores. Hybridization of pathogen DNA and immobilized probes forms a DNA hydrogel by rolling circle amplification and, consequently, blocks the pores to prevent fluid movement, as observed. Following optimization of several factors, including pore size, mesh location, and precision microfluidics, the limit of detection (LOD) for SARS-CoV-2 was determined to be 0.7 aM at 15-min incubation. These results indicate rapid, easy, and effective detection with a moderate-sized LOD of the target pathogen by remote point-of-care testing and without the requirement of any sophisticated device. |
format | Online Article Text |
id | pubmed-7813512 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | The Authors. Published by Elsevier B.V. |
record_format | MEDLINE/PubMed |
spelling | pubmed-78135122021-01-19 A rapid diagnosis of SARS-CoV-2 using DNA hydrogel formation on microfluidic pores Kim, Hwang-soo Abbas, Naseem Shin, Sehyun Biosens Bioelectron Article The coronavirus disease 2019 (COVID-19) pandemic has been a major public health challenge in 2020. Early diagnosis of COVID-19 is the most effective method to control disease spread and prevent further mortality. As such, a high-precision and rapid yet economic assay method is urgently required. Herein, we propose an innovative method to detect severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) using isothermal amplification of nucleic acids on a mesh containing multiple microfluidic pores. Hybridization of pathogen DNA and immobilized probes forms a DNA hydrogel by rolling circle amplification and, consequently, blocks the pores to prevent fluid movement, as observed. Following optimization of several factors, including pore size, mesh location, and precision microfluidics, the limit of detection (LOD) for SARS-CoV-2 was determined to be 0.7 aM at 15-min incubation. These results indicate rapid, easy, and effective detection with a moderate-sized LOD of the target pathogen by remote point-of-care testing and without the requirement of any sophisticated device. The Authors. Published by Elsevier B.V. 2021-04-01 2021-01-18 /pmc/articles/PMC7813512/ /pubmed/33486135 http://dx.doi.org/10.1016/j.bios.2021.113005 Text en © 2021 The Authors Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active. |
spellingShingle | Article Kim, Hwang-soo Abbas, Naseem Shin, Sehyun A rapid diagnosis of SARS-CoV-2 using DNA hydrogel formation on microfluidic pores |
title | A rapid diagnosis of SARS-CoV-2 using DNA hydrogel formation on microfluidic pores |
title_full | A rapid diagnosis of SARS-CoV-2 using DNA hydrogel formation on microfluidic pores |
title_fullStr | A rapid diagnosis of SARS-CoV-2 using DNA hydrogel formation on microfluidic pores |
title_full_unstemmed | A rapid diagnosis of SARS-CoV-2 using DNA hydrogel formation on microfluidic pores |
title_short | A rapid diagnosis of SARS-CoV-2 using DNA hydrogel formation on microfluidic pores |
title_sort | rapid diagnosis of sars-cov-2 using dna hydrogel formation on microfluidic pores |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7813512/ https://www.ncbi.nlm.nih.gov/pubmed/33486135 http://dx.doi.org/10.1016/j.bios.2021.113005 |
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