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Monosexual Cercariae of Schistosoma japonicum Infection Protects Against DSS-Induced Colitis by Shifting the Th1/Th2 Balance and Modulating the Gut Microbiota

Inflammatory bowel disease (IBD)-related inflammation is closely associated with the initiation and progression of colorectal cancer. IBD is generally treated with 5-aminosalicylic acid and immune-modulating medication, but side effects and limitations of these therapies are emerging. Thus, the deve...

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Autores principales: Zhou, Hongli, Zeng, Xiaojing, Sun, Dongchen, Chen, Zhe, Chen, Weixin, Fan, Liwei, Limpanont, Yanin, Dekumyoy, Paron, Maleewong, Wanchai, Lv, Zhiyue
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7813680/
https://www.ncbi.nlm.nih.gov/pubmed/33469451
http://dx.doi.org/10.3389/fmicb.2020.606605
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author Zhou, Hongli
Zeng, Xiaojing
Sun, Dongchen
Chen, Zhe
Chen, Weixin
Fan, Liwei
Limpanont, Yanin
Dekumyoy, Paron
Maleewong, Wanchai
Lv, Zhiyue
author_facet Zhou, Hongli
Zeng, Xiaojing
Sun, Dongchen
Chen, Zhe
Chen, Weixin
Fan, Liwei
Limpanont, Yanin
Dekumyoy, Paron
Maleewong, Wanchai
Lv, Zhiyue
author_sort Zhou, Hongli
collection PubMed
description Inflammatory bowel disease (IBD)-related inflammation is closely associated with the initiation and progression of colorectal cancer. IBD is generally treated with 5-aminosalicylic acid and immune-modulating medication, but side effects and limitations of these therapies are emerging. Thus, the development of novel preventative or therapeutic approaches is imperative. Here, we constructed a dextran sodium sulphate (DSS)-induced IBD mouse model that was infected with monosexual Schistosoma japonicum cercariae (mSjci) at day 1 or administered dexamethasone (DXM) from days 3 to 5 as a positive control. The protective effect of mSjci on IBD mice was evaluated through their assessments of their clinical signs, histopathological lesions and intestinal permeability. To uncover the underlying mechanism, the Th1/Th2 balance and Treg cell population were also examined. Additionally, the alterations in the gut microbiota were assessed to investigate the interaction between the mSjci-modulated immune response and pathogenic microbiome. Mice treated with DSS and mSjci showed fewer IBD clinical signs and less impaired intestinal permeability than DSS-treated mice. Mechanistically, mSjci modulated the Th1/Th2 balance by repressing IFN-γ production, promoting IL-10 expression and enhancing the Treg subset population. Moreover, mSjci notably reshaped the structure, diversity and richness of the gut microbiota community and subsequently exerted immune-modulating effects. Our findings provide evidence showing that mSjci might serve as a novel and effective protective strategy and that the gut microbiota might be a new therapeutic target in IBD.
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spelling pubmed-78136802021-01-18 Monosexual Cercariae of Schistosoma japonicum Infection Protects Against DSS-Induced Colitis by Shifting the Th1/Th2 Balance and Modulating the Gut Microbiota Zhou, Hongli Zeng, Xiaojing Sun, Dongchen Chen, Zhe Chen, Weixin Fan, Liwei Limpanont, Yanin Dekumyoy, Paron Maleewong, Wanchai Lv, Zhiyue Front Microbiol Microbiology Inflammatory bowel disease (IBD)-related inflammation is closely associated with the initiation and progression of colorectal cancer. IBD is generally treated with 5-aminosalicylic acid and immune-modulating medication, but side effects and limitations of these therapies are emerging. Thus, the development of novel preventative or therapeutic approaches is imperative. Here, we constructed a dextran sodium sulphate (DSS)-induced IBD mouse model that was infected with monosexual Schistosoma japonicum cercariae (mSjci) at day 1 or administered dexamethasone (DXM) from days 3 to 5 as a positive control. The protective effect of mSjci on IBD mice was evaluated through their assessments of their clinical signs, histopathological lesions and intestinal permeability. To uncover the underlying mechanism, the Th1/Th2 balance and Treg cell population were also examined. Additionally, the alterations in the gut microbiota were assessed to investigate the interaction between the mSjci-modulated immune response and pathogenic microbiome. Mice treated with DSS and mSjci showed fewer IBD clinical signs and less impaired intestinal permeability than DSS-treated mice. Mechanistically, mSjci modulated the Th1/Th2 balance by repressing IFN-γ production, promoting IL-10 expression and enhancing the Treg subset population. Moreover, mSjci notably reshaped the structure, diversity and richness of the gut microbiota community and subsequently exerted immune-modulating effects. Our findings provide evidence showing that mSjci might serve as a novel and effective protective strategy and that the gut microbiota might be a new therapeutic target in IBD. Frontiers Media S.A. 2021-01-05 /pmc/articles/PMC7813680/ /pubmed/33469451 http://dx.doi.org/10.3389/fmicb.2020.606605 Text en Copyright © 2021 Zhou, Zeng, Sun, Chen, Chen, Fan, Limpanont, Dekumyoy, Maleewong and Lv. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Zhou, Hongli
Zeng, Xiaojing
Sun, Dongchen
Chen, Zhe
Chen, Weixin
Fan, Liwei
Limpanont, Yanin
Dekumyoy, Paron
Maleewong, Wanchai
Lv, Zhiyue
Monosexual Cercariae of Schistosoma japonicum Infection Protects Against DSS-Induced Colitis by Shifting the Th1/Th2 Balance and Modulating the Gut Microbiota
title Monosexual Cercariae of Schistosoma japonicum Infection Protects Against DSS-Induced Colitis by Shifting the Th1/Th2 Balance and Modulating the Gut Microbiota
title_full Monosexual Cercariae of Schistosoma japonicum Infection Protects Against DSS-Induced Colitis by Shifting the Th1/Th2 Balance and Modulating the Gut Microbiota
title_fullStr Monosexual Cercariae of Schistosoma japonicum Infection Protects Against DSS-Induced Colitis by Shifting the Th1/Th2 Balance and Modulating the Gut Microbiota
title_full_unstemmed Monosexual Cercariae of Schistosoma japonicum Infection Protects Against DSS-Induced Colitis by Shifting the Th1/Th2 Balance and Modulating the Gut Microbiota
title_short Monosexual Cercariae of Schistosoma japonicum Infection Protects Against DSS-Induced Colitis by Shifting the Th1/Th2 Balance and Modulating the Gut Microbiota
title_sort monosexual cercariae of schistosoma japonicum infection protects against dss-induced colitis by shifting the th1/th2 balance and modulating the gut microbiota
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7813680/
https://www.ncbi.nlm.nih.gov/pubmed/33469451
http://dx.doi.org/10.3389/fmicb.2020.606605
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