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Cellular Fragments in the Perivitelline Space Are Not a Predictor of Expanded Blastocyst Quality

The presence of cellular fragments in the perivitelline space is a commonly used parameter to determine quality before transfer of in vitro produced (IVP) embryos. However, this parameter is difficult to assess after blastocyst expansion. In this study, we used mechanical hatching to confirm the pre...

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Autores principales: Yu, Bo, van Tol, Helena T. A., Stout, Tom A. E., Roelen, Bernard A. J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7813684/
https://www.ncbi.nlm.nih.gov/pubmed/33469540
http://dx.doi.org/10.3389/fcell.2020.616801
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author Yu, Bo
van Tol, Helena T. A.
Stout, Tom A. E.
Roelen, Bernard A. J.
author_facet Yu, Bo
van Tol, Helena T. A.
Stout, Tom A. E.
Roelen, Bernard A. J.
author_sort Yu, Bo
collection PubMed
description The presence of cellular fragments in the perivitelline space is a commonly used parameter to determine quality before transfer of in vitro produced (IVP) embryos. However, this parameter is difficult to assess after blastocyst expansion. In this study, we used mechanical hatching to confirm the presence of cellular fragments in the perivitelline space of bovine IVP blastocysts. We further looked for associations between possible apoptosis within extruded cells/ cellular fragments and the quality of bovine blastocysts using quantitative RT-PCR and immunofluorescence. Surprisingly, more than 42% of expanded blastocysts had cellular fragments in the perivitelline space; however, more than 37% of extruded cells were TUNEL negative. We observed no significant difference in embryo quality between expanded blastocysts with and without cellular fragments in the perivitelline space. Overall, our data suggest that embryos extrude abnormal cells to maintain their developmental potential. The presence of fragmented cells is not an indicator of embryo quality.
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spelling pubmed-78136842021-01-18 Cellular Fragments in the Perivitelline Space Are Not a Predictor of Expanded Blastocyst Quality Yu, Bo van Tol, Helena T. A. Stout, Tom A. E. Roelen, Bernard A. J. Front Cell Dev Biol Cell and Developmental Biology The presence of cellular fragments in the perivitelline space is a commonly used parameter to determine quality before transfer of in vitro produced (IVP) embryos. However, this parameter is difficult to assess after blastocyst expansion. In this study, we used mechanical hatching to confirm the presence of cellular fragments in the perivitelline space of bovine IVP blastocysts. We further looked for associations between possible apoptosis within extruded cells/ cellular fragments and the quality of bovine blastocysts using quantitative RT-PCR and immunofluorescence. Surprisingly, more than 42% of expanded blastocysts had cellular fragments in the perivitelline space; however, more than 37% of extruded cells were TUNEL negative. We observed no significant difference in embryo quality between expanded blastocysts with and without cellular fragments in the perivitelline space. Overall, our data suggest that embryos extrude abnormal cells to maintain their developmental potential. The presence of fragmented cells is not an indicator of embryo quality. Frontiers Media S.A. 2021-01-05 /pmc/articles/PMC7813684/ /pubmed/33469540 http://dx.doi.org/10.3389/fcell.2020.616801 Text en Copyright © 2021 Yu, van Tol, Stout and Roelen. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cell and Developmental Biology
Yu, Bo
van Tol, Helena T. A.
Stout, Tom A. E.
Roelen, Bernard A. J.
Cellular Fragments in the Perivitelline Space Are Not a Predictor of Expanded Blastocyst Quality
title Cellular Fragments in the Perivitelline Space Are Not a Predictor of Expanded Blastocyst Quality
title_full Cellular Fragments in the Perivitelline Space Are Not a Predictor of Expanded Blastocyst Quality
title_fullStr Cellular Fragments in the Perivitelline Space Are Not a Predictor of Expanded Blastocyst Quality
title_full_unstemmed Cellular Fragments in the Perivitelline Space Are Not a Predictor of Expanded Blastocyst Quality
title_short Cellular Fragments in the Perivitelline Space Are Not a Predictor of Expanded Blastocyst Quality
title_sort cellular fragments in the perivitelline space are not a predictor of expanded blastocyst quality
topic Cell and Developmental Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7813684/
https://www.ncbi.nlm.nih.gov/pubmed/33469540
http://dx.doi.org/10.3389/fcell.2020.616801
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