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Lead (Pb) Accumulation in Human THP-1 Monocytes/Macrophages In Vitro and the Influence on Cell Apoptosis

In this study, we investigated the ability of THP-1 monocytes and macrophages to accumulate lead (Pb) in vitro, relative to Pb concentration and length of exposure. Moreover, we also evaluated the effect of Pb accumulation on cell viability and apoptosis. THP-1 monocytes and macrophages were culture...

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Autores principales: Metryka, Emilia, Kupnicka, Patrycja, Kapczuk, Patrycja, Aszakiewicz, Beata, Piotrowska, Katarzyna, Tkacz, Marta, Gutowska, Izabela, Chlubek, Dariusz, Baranowska-Bosiacka, Irena
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer US 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7813697/
https://www.ncbi.nlm.nih.gov/pubmed/32557104
http://dx.doi.org/10.1007/s12011-020-02215-7
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author Metryka, Emilia
Kupnicka, Patrycja
Kapczuk, Patrycja
Aszakiewicz, Beata
Piotrowska, Katarzyna
Tkacz, Marta
Gutowska, Izabela
Chlubek, Dariusz
Baranowska-Bosiacka, Irena
author_facet Metryka, Emilia
Kupnicka, Patrycja
Kapczuk, Patrycja
Aszakiewicz, Beata
Piotrowska, Katarzyna
Tkacz, Marta
Gutowska, Izabela
Chlubek, Dariusz
Baranowska-Bosiacka, Irena
author_sort Metryka, Emilia
collection PubMed
description In this study, we investigated the ability of THP-1 monocytes and macrophages to accumulate lead (Pb) in vitro, relative to Pb concentration and length of exposure. Moreover, we also evaluated the effect of Pb accumulation on cell viability and apoptosis. THP-1 monocytes and macrophages were cultured in the presence of Pb at 1.25 μg/dL, 2.5 μg/dL, 5 μg/dL, and 10 μg/dL. Pb accumulation was examined by inductively coupled plasma and confocal microscopy. The influence of Pb on cell viability, apoptosis, and necrosis was assessed using flow cytometry. The results showed that Pb was toxic to THP-1 monocytes/macrophages even at very low environmental concentrations. Despite the use of low concentrations, both monocytes and macrophages showed dose-dependent and time-dependent decreases in viability, with a simultaneous increase in the percentage of early and late apoptotic cells. Macrophages reacted more strongly to Pb than monocytes. When exposed to the same Pb concentrations, they showed lower viability and a higher percentage of necrotic cells. The incubation time positively correlated with Pb accumulation in a dose-dependent manner. The obtained results indicate that environmental exposure to low Pb concentrations may significantly impair the function of macrophages, with the increased number of apoptotic cells potentially contributing to the development of many pathologies in the brain and whole body.
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spelling pubmed-78136972021-01-25 Lead (Pb) Accumulation in Human THP-1 Monocytes/Macrophages In Vitro and the Influence on Cell Apoptosis Metryka, Emilia Kupnicka, Patrycja Kapczuk, Patrycja Aszakiewicz, Beata Piotrowska, Katarzyna Tkacz, Marta Gutowska, Izabela Chlubek, Dariusz Baranowska-Bosiacka, Irena Biol Trace Elem Res Article In this study, we investigated the ability of THP-1 monocytes and macrophages to accumulate lead (Pb) in vitro, relative to Pb concentration and length of exposure. Moreover, we also evaluated the effect of Pb accumulation on cell viability and apoptosis. THP-1 monocytes and macrophages were cultured in the presence of Pb at 1.25 μg/dL, 2.5 μg/dL, 5 μg/dL, and 10 μg/dL. Pb accumulation was examined by inductively coupled plasma and confocal microscopy. The influence of Pb on cell viability, apoptosis, and necrosis was assessed using flow cytometry. The results showed that Pb was toxic to THP-1 monocytes/macrophages even at very low environmental concentrations. Despite the use of low concentrations, both monocytes and macrophages showed dose-dependent and time-dependent decreases in viability, with a simultaneous increase in the percentage of early and late apoptotic cells. Macrophages reacted more strongly to Pb than monocytes. When exposed to the same Pb concentrations, they showed lower viability and a higher percentage of necrotic cells. The incubation time positively correlated with Pb accumulation in a dose-dependent manner. The obtained results indicate that environmental exposure to low Pb concentrations may significantly impair the function of macrophages, with the increased number of apoptotic cells potentially contributing to the development of many pathologies in the brain and whole body. Springer US 2020-06-15 2021 /pmc/articles/PMC7813697/ /pubmed/32557104 http://dx.doi.org/10.1007/s12011-020-02215-7 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Metryka, Emilia
Kupnicka, Patrycja
Kapczuk, Patrycja
Aszakiewicz, Beata
Piotrowska, Katarzyna
Tkacz, Marta
Gutowska, Izabela
Chlubek, Dariusz
Baranowska-Bosiacka, Irena
Lead (Pb) Accumulation in Human THP-1 Monocytes/Macrophages In Vitro and the Influence on Cell Apoptosis
title Lead (Pb) Accumulation in Human THP-1 Monocytes/Macrophages In Vitro and the Influence on Cell Apoptosis
title_full Lead (Pb) Accumulation in Human THP-1 Monocytes/Macrophages In Vitro and the Influence on Cell Apoptosis
title_fullStr Lead (Pb) Accumulation in Human THP-1 Monocytes/Macrophages In Vitro and the Influence on Cell Apoptosis
title_full_unstemmed Lead (Pb) Accumulation in Human THP-1 Monocytes/Macrophages In Vitro and the Influence on Cell Apoptosis
title_short Lead (Pb) Accumulation in Human THP-1 Monocytes/Macrophages In Vitro and the Influence on Cell Apoptosis
title_sort lead (pb) accumulation in human thp-1 monocytes/macrophages in vitro and the influence on cell apoptosis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7813697/
https://www.ncbi.nlm.nih.gov/pubmed/32557104
http://dx.doi.org/10.1007/s12011-020-02215-7
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