Cargando…

Design of DOX-GNRs-PNIPAM@PEG-PLA Micelle With Temperature and Light Dual-Function for Potent Melanoma Therapy

Objective: The aim of this study was to construct light and temperature dual-sensitive micellar carriers loaded with doxorubicin (DOX) and gold nanorods (DOX-GNRs-PNIPAM@PEG-PLA, DAPP) for melanoma therapy. Methods: The DAPP self-assembled using fine-tuned physicochemical properties in water. The DA...

Descripción completa

Detalles Bibliográficos
Autores principales: Wang, Na, Shi, Jing, Wu, Cong, Chu, Weiwei, Tao, Wanru, Li, Wei, Yuan, Xiaohai
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7813802/
https://www.ncbi.nlm.nih.gov/pubmed/33469525
http://dx.doi.org/10.3389/fchem.2020.599740
_version_ 1783637930955243520
author Wang, Na
Shi, Jing
Wu, Cong
Chu, Weiwei
Tao, Wanru
Li, Wei
Yuan, Xiaohai
author_facet Wang, Na
Shi, Jing
Wu, Cong
Chu, Weiwei
Tao, Wanru
Li, Wei
Yuan, Xiaohai
author_sort Wang, Na
collection PubMed
description Objective: The aim of this study was to construct light and temperature dual-sensitive micellar carriers loaded with doxorubicin (DOX) and gold nanorods (DOX-GNRs-PNIPAM@PEG-PLA, DAPP) for melanoma therapy. Methods: The DAPP self-assembled using fine-tuned physicochemical properties in water. The DAPP structure, temperature- and photo-sensitivity, drug-release, in-vitro serum stability, and cytotoxicity against melanoma B16F10 cells were evaluated in detail. The corresponding in-vitro and in-vivo therapeutic mechanisms were then evaluated using a B16F10-melanoma bearing BALB/c nude mouse model (B16F10). Results: The light and temperature sensitive micellar drug-delivery system assembled from block copolymer and gold nanorods exhibited a narrow particle size and size distribution, good biocompatibility, well-designed photo-temperature conversion, controlled drug release, and high serum stability. Compared with the free DOX- and PBS-treated groups, the cell endocytosis-mediated cytotoxicity and intra-tumor accumulation of DAPP was markedly enhanced by the NIR-light exposure and induced potent in-vivo tumor inhibitory activity. Conclusion: The design of DAPP, a dual-functional micellar drug-delivery system with temperature- and light-sensitive properties, offers a new strategy for skin-cancer therapy with a potent therapeutic index.
format Online
Article
Text
id pubmed-7813802
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-78138022021-01-18 Design of DOX-GNRs-PNIPAM@PEG-PLA Micelle With Temperature and Light Dual-Function for Potent Melanoma Therapy Wang, Na Shi, Jing Wu, Cong Chu, Weiwei Tao, Wanru Li, Wei Yuan, Xiaohai Front Chem Chemistry Objective: The aim of this study was to construct light and temperature dual-sensitive micellar carriers loaded with doxorubicin (DOX) and gold nanorods (DOX-GNRs-PNIPAM@PEG-PLA, DAPP) for melanoma therapy. Methods: The DAPP self-assembled using fine-tuned physicochemical properties in water. The DAPP structure, temperature- and photo-sensitivity, drug-release, in-vitro serum stability, and cytotoxicity against melanoma B16F10 cells were evaluated in detail. The corresponding in-vitro and in-vivo therapeutic mechanisms were then evaluated using a B16F10-melanoma bearing BALB/c nude mouse model (B16F10). Results: The light and temperature sensitive micellar drug-delivery system assembled from block copolymer and gold nanorods exhibited a narrow particle size and size distribution, good biocompatibility, well-designed photo-temperature conversion, controlled drug release, and high serum stability. Compared with the free DOX- and PBS-treated groups, the cell endocytosis-mediated cytotoxicity and intra-tumor accumulation of DAPP was markedly enhanced by the NIR-light exposure and induced potent in-vivo tumor inhibitory activity. Conclusion: The design of DAPP, a dual-functional micellar drug-delivery system with temperature- and light-sensitive properties, offers a new strategy for skin-cancer therapy with a potent therapeutic index. Frontiers Media S.A. 2021-01-05 /pmc/articles/PMC7813802/ /pubmed/33469525 http://dx.doi.org/10.3389/fchem.2020.599740 Text en Copyright © 2021 Wang, Shi, Wu, Chu, Tao, Li and Yuan. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Chemistry
Wang, Na
Shi, Jing
Wu, Cong
Chu, Weiwei
Tao, Wanru
Li, Wei
Yuan, Xiaohai
Design of DOX-GNRs-PNIPAM@PEG-PLA Micelle With Temperature and Light Dual-Function for Potent Melanoma Therapy
title Design of DOX-GNRs-PNIPAM@PEG-PLA Micelle With Temperature and Light Dual-Function for Potent Melanoma Therapy
title_full Design of DOX-GNRs-PNIPAM@PEG-PLA Micelle With Temperature and Light Dual-Function for Potent Melanoma Therapy
title_fullStr Design of DOX-GNRs-PNIPAM@PEG-PLA Micelle With Temperature and Light Dual-Function for Potent Melanoma Therapy
title_full_unstemmed Design of DOX-GNRs-PNIPAM@PEG-PLA Micelle With Temperature and Light Dual-Function for Potent Melanoma Therapy
title_short Design of DOX-GNRs-PNIPAM@PEG-PLA Micelle With Temperature and Light Dual-Function for Potent Melanoma Therapy
title_sort design of dox-gnrs-pnipam@peg-pla micelle with temperature and light dual-function for potent melanoma therapy
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7813802/
https://www.ncbi.nlm.nih.gov/pubmed/33469525
http://dx.doi.org/10.3389/fchem.2020.599740
work_keys_str_mv AT wangna designofdoxgnrspnipampegplamicellewithtemperatureandlightdualfunctionforpotentmelanomatherapy
AT shijing designofdoxgnrspnipampegplamicellewithtemperatureandlightdualfunctionforpotentmelanomatherapy
AT wucong designofdoxgnrspnipampegplamicellewithtemperatureandlightdualfunctionforpotentmelanomatherapy
AT chuweiwei designofdoxgnrspnipampegplamicellewithtemperatureandlightdualfunctionforpotentmelanomatherapy
AT taowanru designofdoxgnrspnipampegplamicellewithtemperatureandlightdualfunctionforpotentmelanomatherapy
AT liwei designofdoxgnrspnipampegplamicellewithtemperatureandlightdualfunctionforpotentmelanomatherapy
AT yuanxiaohai designofdoxgnrspnipampegplamicellewithtemperatureandlightdualfunctionforpotentmelanomatherapy