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Development of a robust hydroponic method for screening of sunflower (Helianthus annuus L.) accessions for tolerance to heat and osmotic stress
Hydroponic systems are known to provide a platform for uniform growth conditions until the reproductive stage. However, many plant species, including sunflower, show poor growth and survivability under conventional hydroponic systems due to poor nutrient availability, hypoxia and algal contamination...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7814013/ https://www.ncbi.nlm.nih.gov/pubmed/33462271 http://dx.doi.org/10.1038/s41598-021-81072-3 |
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author | Shehzad, Muhammad Gul, Rao Samran Rauf, Saeed Clarindo, Wellington Ronildo Al-Khayri, Jameel Mohammed Hussain, Muhammad Mubashar Munir, Hassan Ghaffari, Mehdi Nazir, Shahid Hussain, Majid |
author_facet | Shehzad, Muhammad Gul, Rao Samran Rauf, Saeed Clarindo, Wellington Ronildo Al-Khayri, Jameel Mohammed Hussain, Muhammad Mubashar Munir, Hassan Ghaffari, Mehdi Nazir, Shahid Hussain, Majid |
author_sort | Shehzad, Muhammad |
collection | PubMed |
description | Hydroponic systems are known to provide a platform for uniform growth conditions until the reproductive stage. However, many plant species, including sunflower, show poor growth and survivability under conventional hydroponic systems due to poor nutrient availability, hypoxia and algal contamination. Thus, we tested various hydroponic systems to select a hydroponic system suitable for screening of sunflower germplasm. Sunflower accessions showed better growth and leaf gas exchange in newly-designed over conventional hydroponic systems. Selected hydroponic systems were further engaged in sunflower accession screening under heat and osmotic stress in a two-pan system (210 cm × 60 cm). Heat stress treatment was applied by growing sunflower germplasm at 42 °C and osmotic stress by adding polyethylene glycol 8000 which decreased the osmotic potential to − 0.6 MPa. There was significant variability among the sunflower accessions for their ability to survive under stress. Accessions such as C-2721 (43%), C-291 (46%) and D-14 (43%) had lower cell membrane injury percentage under osmotic stress and high seedling survivability (60‒80%) under heat stress when compared with susceptible accessions. Moreover, resistant accessions exhibited greater cuticular waxes and root length but lower transpiration losses. The newly designed hydroponic platform proved reliable for the selection of resistant sunflower accessions. Selected parental lines were validated by assessing their hybrids under field trials across two seasons under water and temperature stress during the reproductive phase (autumn). Hybrid H3 obtained by crossing drought and heat resistant parents had the highest seed yield and water use efficiency. |
format | Online Article Text |
id | pubmed-7814013 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-78140132021-01-21 Development of a robust hydroponic method for screening of sunflower (Helianthus annuus L.) accessions for tolerance to heat and osmotic stress Shehzad, Muhammad Gul, Rao Samran Rauf, Saeed Clarindo, Wellington Ronildo Al-Khayri, Jameel Mohammed Hussain, Muhammad Mubashar Munir, Hassan Ghaffari, Mehdi Nazir, Shahid Hussain, Majid Sci Rep Article Hydroponic systems are known to provide a platform for uniform growth conditions until the reproductive stage. However, many plant species, including sunflower, show poor growth and survivability under conventional hydroponic systems due to poor nutrient availability, hypoxia and algal contamination. Thus, we tested various hydroponic systems to select a hydroponic system suitable for screening of sunflower germplasm. Sunflower accessions showed better growth and leaf gas exchange in newly-designed over conventional hydroponic systems. Selected hydroponic systems were further engaged in sunflower accession screening under heat and osmotic stress in a two-pan system (210 cm × 60 cm). Heat stress treatment was applied by growing sunflower germplasm at 42 °C and osmotic stress by adding polyethylene glycol 8000 which decreased the osmotic potential to − 0.6 MPa. There was significant variability among the sunflower accessions for their ability to survive under stress. Accessions such as C-2721 (43%), C-291 (46%) and D-14 (43%) had lower cell membrane injury percentage under osmotic stress and high seedling survivability (60‒80%) under heat stress when compared with susceptible accessions. Moreover, resistant accessions exhibited greater cuticular waxes and root length but lower transpiration losses. The newly designed hydroponic platform proved reliable for the selection of resistant sunflower accessions. Selected parental lines were validated by assessing their hybrids under field trials across two seasons under water and temperature stress during the reproductive phase (autumn). Hybrid H3 obtained by crossing drought and heat resistant parents had the highest seed yield and water use efficiency. Nature Publishing Group UK 2021-01-18 /pmc/articles/PMC7814013/ /pubmed/33462271 http://dx.doi.org/10.1038/s41598-021-81072-3 Text en © The Author(s) 2021 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Shehzad, Muhammad Gul, Rao Samran Rauf, Saeed Clarindo, Wellington Ronildo Al-Khayri, Jameel Mohammed Hussain, Muhammad Mubashar Munir, Hassan Ghaffari, Mehdi Nazir, Shahid Hussain, Majid Development of a robust hydroponic method for screening of sunflower (Helianthus annuus L.) accessions for tolerance to heat and osmotic stress |
title | Development of a robust hydroponic method for screening of sunflower (Helianthus annuus L.) accessions for tolerance to heat and osmotic stress |
title_full | Development of a robust hydroponic method for screening of sunflower (Helianthus annuus L.) accessions for tolerance to heat and osmotic stress |
title_fullStr | Development of a robust hydroponic method for screening of sunflower (Helianthus annuus L.) accessions for tolerance to heat and osmotic stress |
title_full_unstemmed | Development of a robust hydroponic method for screening of sunflower (Helianthus annuus L.) accessions for tolerance to heat and osmotic stress |
title_short | Development of a robust hydroponic method for screening of sunflower (Helianthus annuus L.) accessions for tolerance to heat and osmotic stress |
title_sort | development of a robust hydroponic method for screening of sunflower (helianthus annuus l.) accessions for tolerance to heat and osmotic stress |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7814013/ https://www.ncbi.nlm.nih.gov/pubmed/33462271 http://dx.doi.org/10.1038/s41598-021-81072-3 |
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