STED lithography in microfluidics for 3D thrombocyte aggregation testing

Three-dimensional photopolymerization techniques such as multiphoton polymerization lithography (MPL) and stimulated emission depletion (STED) lithography are powerful tools for fabricating structures in the sub-µm range. Combining these techniques with microfluidics enables us to broaden the range...

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Autores principales: Buchegger, Bianca, Tanzer, Alexander, Posch, Sandra, Gabriel, Christian, Klar, Thomas A., Jacak, Jaroslaw
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2021
Materias:
Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7814651/
https://www.ncbi.nlm.nih.gov/pubmed/33461577
http://dx.doi.org/10.1186/s12951-020-00762-8
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author Buchegger, Bianca
Tanzer, Alexander
Posch, Sandra
Gabriel, Christian
Klar, Thomas A.
Jacak, Jaroslaw
author_facet Buchegger, Bianca
Tanzer, Alexander
Posch, Sandra
Gabriel, Christian
Klar, Thomas A.
Jacak, Jaroslaw
author_sort Buchegger, Bianca
collection PubMed
description Three-dimensional photopolymerization techniques such as multiphoton polymerization lithography (MPL) and stimulated emission depletion (STED) lithography are powerful tools for fabricating structures in the sub-µm range. Combining these techniques with microfluidics enables us to broaden the range of their applications. In this study, we show a microfluidic device enhanced with MPL structures carrying STED-lithographically written nanoanchors that promote binding of the von Willebrand factor (vWF). The density of vWF is adjusted by varying the number of the nanoanchors on the 3D structures. This allows us to study the impact of the density of vWF on the activation of thrombocytes. The activation of the thrombocytes seems to decrease with the density of vWF on the 3D scaffolds inside the microfluidic channels. [Image: see text]
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spelling pubmed-78146512021-01-19 STED lithography in microfluidics for 3D thrombocyte aggregation testing Buchegger, Bianca Tanzer, Alexander Posch, Sandra Gabriel, Christian Klar, Thomas A. Jacak, Jaroslaw J Nanobiotechnology Short Communication Three-dimensional photopolymerization techniques such as multiphoton polymerization lithography (MPL) and stimulated emission depletion (STED) lithography are powerful tools for fabricating structures in the sub-µm range. Combining these techniques with microfluidics enables us to broaden the range of their applications. In this study, we show a microfluidic device enhanced with MPL structures carrying STED-lithographically written nanoanchors that promote binding of the von Willebrand factor (vWF). The density of vWF is adjusted by varying the number of the nanoanchors on the 3D structures. This allows us to study the impact of the density of vWF on the activation of thrombocytes. The activation of the thrombocytes seems to decrease with the density of vWF on the 3D scaffolds inside the microfluidic channels. [Image: see text] BioMed Central 2021-01-18 /pmc/articles/PMC7814651/ /pubmed/33461577 http://dx.doi.org/10.1186/s12951-020-00762-8 Text en © The Author(s) 2021 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Short Communication
Buchegger, Bianca
Tanzer, Alexander
Posch, Sandra
Gabriel, Christian
Klar, Thomas A.
Jacak, Jaroslaw
STED lithography in microfluidics for 3D thrombocyte aggregation testing
title STED lithography in microfluidics for 3D thrombocyte aggregation testing
title_full STED lithography in microfluidics for 3D thrombocyte aggregation testing
title_fullStr STED lithography in microfluidics for 3D thrombocyte aggregation testing
title_full_unstemmed STED lithography in microfluidics for 3D thrombocyte aggregation testing
title_short STED lithography in microfluidics for 3D thrombocyte aggregation testing
title_sort sted lithography in microfluidics for 3d thrombocyte aggregation testing
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7814651/
https://www.ncbi.nlm.nih.gov/pubmed/33461577
http://dx.doi.org/10.1186/s12951-020-00762-8
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