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Microtubule‐assisted mechanism for toxisome assembly in Fusarium graminearum

In Fusarium graminearum, a trichothecene biosynthetic complex known as the toxisome forms ovoid and spherical structures in the remodelled endoplasmic reticulum (ER) under mycotoxin‐inducing conditions. Previous studies also demonstrated that disruption of actin and tubulin results in a significant...

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Autores principales: Zhou, Zehua, Duan, Yabing, Zhang, Jie, Lu, Fei, Zhu, Yuanye, Shim, Won Bo, Zhou, Mingguo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7814972/
https://www.ncbi.nlm.nih.gov/pubmed/33201575
http://dx.doi.org/10.1111/mpp.13015
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author Zhou, Zehua
Duan, Yabing
Zhang, Jie
Lu, Fei
Zhu, Yuanye
Shim, Won Bo
Zhou, Mingguo
author_facet Zhou, Zehua
Duan, Yabing
Zhang, Jie
Lu, Fei
Zhu, Yuanye
Shim, Won Bo
Zhou, Mingguo
author_sort Zhou, Zehua
collection PubMed
description In Fusarium graminearum, a trichothecene biosynthetic complex known as the toxisome forms ovoid and spherical structures in the remodelled endoplasmic reticulum (ER) under mycotoxin‐inducing conditions. Previous studies also demonstrated that disruption of actin and tubulin results in a significant decrease in deoxynivalenol (DON) biosynthesis in F. graminearum. However, the functional association between the toxisome and microtubule components has not been clearly defined. In this study we tested the hypothesis that the microtubule network provides key support for toxisome assembly and thus facilitates DON biosynthesis. Through fluorescent live cell imaging, knockout mutant generation, and protein–protein interaction assays, we determined that two of the four F. graminearum tubulins, α(1) and β(2) tubulins, are indispensable for DON production. We also showed that these two tubulins are directly associated. When the α(1)–β(2) tubulin heterodimer is disrupted, the metabolic activity of the toxisome is significantly suppressed, which leads to significant DON biosynthesis impairment. Similar phenotypic outcomes were shown when F. graminearum wild type was treated with carbendazim, a fungicide that binds to microtubules and disrupts spindle formation. Based on our results, we propose a model where α(1)–β(2) tubulin heterodimer serves as the scaffold for functional toxisome assembly in F. graminearum.
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spelling pubmed-78149722021-01-27 Microtubule‐assisted mechanism for toxisome assembly in Fusarium graminearum Zhou, Zehua Duan, Yabing Zhang, Jie Lu, Fei Zhu, Yuanye Shim, Won Bo Zhou, Mingguo Mol Plant Pathol Original Articles In Fusarium graminearum, a trichothecene biosynthetic complex known as the toxisome forms ovoid and spherical structures in the remodelled endoplasmic reticulum (ER) under mycotoxin‐inducing conditions. Previous studies also demonstrated that disruption of actin and tubulin results in a significant decrease in deoxynivalenol (DON) biosynthesis in F. graminearum. However, the functional association between the toxisome and microtubule components has not been clearly defined. In this study we tested the hypothesis that the microtubule network provides key support for toxisome assembly and thus facilitates DON biosynthesis. Through fluorescent live cell imaging, knockout mutant generation, and protein–protein interaction assays, we determined that two of the four F. graminearum tubulins, α(1) and β(2) tubulins, are indispensable for DON production. We also showed that these two tubulins are directly associated. When the α(1)–β(2) tubulin heterodimer is disrupted, the metabolic activity of the toxisome is significantly suppressed, which leads to significant DON biosynthesis impairment. Similar phenotypic outcomes were shown when F. graminearum wild type was treated with carbendazim, a fungicide that binds to microtubules and disrupts spindle formation. Based on our results, we propose a model where α(1)–β(2) tubulin heterodimer serves as the scaffold for functional toxisome assembly in F. graminearum. John Wiley and Sons Inc. 2020-11-17 /pmc/articles/PMC7814972/ /pubmed/33201575 http://dx.doi.org/10.1111/mpp.13015 Text en © 2020 The Authors. Molecular Plant Pathology published by British Society for Plant Pathology and John Wiley & Sons Ltd This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Articles
Zhou, Zehua
Duan, Yabing
Zhang, Jie
Lu, Fei
Zhu, Yuanye
Shim, Won Bo
Zhou, Mingguo
Microtubule‐assisted mechanism for toxisome assembly in Fusarium graminearum
title Microtubule‐assisted mechanism for toxisome assembly in Fusarium graminearum
title_full Microtubule‐assisted mechanism for toxisome assembly in Fusarium graminearum
title_fullStr Microtubule‐assisted mechanism for toxisome assembly in Fusarium graminearum
title_full_unstemmed Microtubule‐assisted mechanism for toxisome assembly in Fusarium graminearum
title_short Microtubule‐assisted mechanism for toxisome assembly in Fusarium graminearum
title_sort microtubule‐assisted mechanism for toxisome assembly in fusarium graminearum
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7814972/
https://www.ncbi.nlm.nih.gov/pubmed/33201575
http://dx.doi.org/10.1111/mpp.13015
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