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Glutamine and cystine-enriched diets modulate aquaporins gene expression in the small intestine of piglets

The regulation of glycerol permeability in the gastrointestinal tract is crucial to control fat deposition, lipolysis and gluconeogenesis. Knowing that the amino acid glutamine is a physiological regulator of gluconeogenesis, whereas cystine promotes adiposity, herein we investigated the effects of...

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Autores principales: Vieira da Silva, Inês, P. Soares, Bárbara, Pimpão, Catarina, M. A. Pinto, Rui, Costa, Teresa, Freire, João P. B., Corrent, Etienne, Chalvon-Demersay, Tristan, Prates, José A. M., Lopes, Paula A., Soveral, Graça
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7815100/
https://www.ncbi.nlm.nih.gov/pubmed/33465153
http://dx.doi.org/10.1371/journal.pone.0245739
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author Vieira da Silva, Inês
P. Soares, Bárbara
Pimpão, Catarina
M. A. Pinto, Rui
Costa, Teresa
Freire, João P. B.
Corrent, Etienne
Chalvon-Demersay, Tristan
Prates, José A. M.
Lopes, Paula A.
Soveral, Graça
author_facet Vieira da Silva, Inês
P. Soares, Bárbara
Pimpão, Catarina
M. A. Pinto, Rui
Costa, Teresa
Freire, João P. B.
Corrent, Etienne
Chalvon-Demersay, Tristan
Prates, José A. M.
Lopes, Paula A.
Soveral, Graça
author_sort Vieira da Silva, Inês
collection PubMed
description The regulation of glycerol permeability in the gastrointestinal tract is crucial to control fat deposition, lipolysis and gluconeogenesis. Knowing that the amino acid glutamine is a physiological regulator of gluconeogenesis, whereas cystine promotes adiposity, herein we investigated the effects of dietary supplementation with glutamine and cystine on the serum biochemical parameters of piglets fed on amino acid-enriched diets, as well as on the transcriptional profile of membrane water and glycerol channels aquaporins (AQPs) in the ileum portion of the small intestine and its impact on intestinal permeability. Twenty male piglets with an initial body weight of 8.8 ± 0.89 kg were allocated to four dietary treatments (n = 5) and received, during a four week-period, a basal diet without supplementation (control) or supplemented with 8 kg/ton of glutamine (Gln), cystine (Cys) or the combination of the two amino acids in equal proportions (Gln + Cys). Most biochemical parameters were found improved in piglets fed Gln and Cys diet. mRNA levels of AQP3 were found predominant over the others. Both amino acids, individually or combined, were responsible for a consistent downregulation of AQP1, AQP7 and AQP10, without impacting on water permeability. Conversely, Cys enriched diet upregulated AQP3 enhancing basolateral membranes glycerol permeability and downregulating glycerol kinase (GK) of intestinal cells. Altogether, our data reveal that amino acids dietary supplementation can modulate intestinal AQPs expression and unveil AQP3 as a promising target for adipogenesis regulation.
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spelling pubmed-78151002021-01-27 Glutamine and cystine-enriched diets modulate aquaporins gene expression in the small intestine of piglets Vieira da Silva, Inês P. Soares, Bárbara Pimpão, Catarina M. A. Pinto, Rui Costa, Teresa Freire, João P. B. Corrent, Etienne Chalvon-Demersay, Tristan Prates, José A. M. Lopes, Paula A. Soveral, Graça PLoS One Research Article The regulation of glycerol permeability in the gastrointestinal tract is crucial to control fat deposition, lipolysis and gluconeogenesis. Knowing that the amino acid glutamine is a physiological regulator of gluconeogenesis, whereas cystine promotes adiposity, herein we investigated the effects of dietary supplementation with glutamine and cystine on the serum biochemical parameters of piglets fed on amino acid-enriched diets, as well as on the transcriptional profile of membrane water and glycerol channels aquaporins (AQPs) in the ileum portion of the small intestine and its impact on intestinal permeability. Twenty male piglets with an initial body weight of 8.8 ± 0.89 kg were allocated to four dietary treatments (n = 5) and received, during a four week-period, a basal diet without supplementation (control) or supplemented with 8 kg/ton of glutamine (Gln), cystine (Cys) or the combination of the two amino acids in equal proportions (Gln + Cys). Most biochemical parameters were found improved in piglets fed Gln and Cys diet. mRNA levels of AQP3 were found predominant over the others. Both amino acids, individually or combined, were responsible for a consistent downregulation of AQP1, AQP7 and AQP10, without impacting on water permeability. Conversely, Cys enriched diet upregulated AQP3 enhancing basolateral membranes glycerol permeability and downregulating glycerol kinase (GK) of intestinal cells. Altogether, our data reveal that amino acids dietary supplementation can modulate intestinal AQPs expression and unveil AQP3 as a promising target for adipogenesis regulation. Public Library of Science 2021-01-19 /pmc/articles/PMC7815100/ /pubmed/33465153 http://dx.doi.org/10.1371/journal.pone.0245739 Text en © 2021 Vieira da Silva et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Vieira da Silva, Inês
P. Soares, Bárbara
Pimpão, Catarina
M. A. Pinto, Rui
Costa, Teresa
Freire, João P. B.
Corrent, Etienne
Chalvon-Demersay, Tristan
Prates, José A. M.
Lopes, Paula A.
Soveral, Graça
Glutamine and cystine-enriched diets modulate aquaporins gene expression in the small intestine of piglets
title Glutamine and cystine-enriched diets modulate aquaporins gene expression in the small intestine of piglets
title_full Glutamine and cystine-enriched diets modulate aquaporins gene expression in the small intestine of piglets
title_fullStr Glutamine and cystine-enriched diets modulate aquaporins gene expression in the small intestine of piglets
title_full_unstemmed Glutamine and cystine-enriched diets modulate aquaporins gene expression in the small intestine of piglets
title_short Glutamine and cystine-enriched diets modulate aquaporins gene expression in the small intestine of piglets
title_sort glutamine and cystine-enriched diets modulate aquaporins gene expression in the small intestine of piglets
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7815100/
https://www.ncbi.nlm.nih.gov/pubmed/33465153
http://dx.doi.org/10.1371/journal.pone.0245739
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