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Glutamine and cystine-enriched diets modulate aquaporins gene expression in the small intestine of piglets
The regulation of glycerol permeability in the gastrointestinal tract is crucial to control fat deposition, lipolysis and gluconeogenesis. Knowing that the amino acid glutamine is a physiological regulator of gluconeogenesis, whereas cystine promotes adiposity, herein we investigated the effects of...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7815100/ https://www.ncbi.nlm.nih.gov/pubmed/33465153 http://dx.doi.org/10.1371/journal.pone.0245739 |
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author | Vieira da Silva, Inês P. Soares, Bárbara Pimpão, Catarina M. A. Pinto, Rui Costa, Teresa Freire, João P. B. Corrent, Etienne Chalvon-Demersay, Tristan Prates, José A. M. Lopes, Paula A. Soveral, Graça |
author_facet | Vieira da Silva, Inês P. Soares, Bárbara Pimpão, Catarina M. A. Pinto, Rui Costa, Teresa Freire, João P. B. Corrent, Etienne Chalvon-Demersay, Tristan Prates, José A. M. Lopes, Paula A. Soveral, Graça |
author_sort | Vieira da Silva, Inês |
collection | PubMed |
description | The regulation of glycerol permeability in the gastrointestinal tract is crucial to control fat deposition, lipolysis and gluconeogenesis. Knowing that the amino acid glutamine is a physiological regulator of gluconeogenesis, whereas cystine promotes adiposity, herein we investigated the effects of dietary supplementation with glutamine and cystine on the serum biochemical parameters of piglets fed on amino acid-enriched diets, as well as on the transcriptional profile of membrane water and glycerol channels aquaporins (AQPs) in the ileum portion of the small intestine and its impact on intestinal permeability. Twenty male piglets with an initial body weight of 8.8 ± 0.89 kg were allocated to four dietary treatments (n = 5) and received, during a four week-period, a basal diet without supplementation (control) or supplemented with 8 kg/ton of glutamine (Gln), cystine (Cys) or the combination of the two amino acids in equal proportions (Gln + Cys). Most biochemical parameters were found improved in piglets fed Gln and Cys diet. mRNA levels of AQP3 were found predominant over the others. Both amino acids, individually or combined, were responsible for a consistent downregulation of AQP1, AQP7 and AQP10, without impacting on water permeability. Conversely, Cys enriched diet upregulated AQP3 enhancing basolateral membranes glycerol permeability and downregulating glycerol kinase (GK) of intestinal cells. Altogether, our data reveal that amino acids dietary supplementation can modulate intestinal AQPs expression and unveil AQP3 as a promising target for adipogenesis regulation. |
format | Online Article Text |
id | pubmed-7815100 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-78151002021-01-27 Glutamine and cystine-enriched diets modulate aquaporins gene expression in the small intestine of piglets Vieira da Silva, Inês P. Soares, Bárbara Pimpão, Catarina M. A. Pinto, Rui Costa, Teresa Freire, João P. B. Corrent, Etienne Chalvon-Demersay, Tristan Prates, José A. M. Lopes, Paula A. Soveral, Graça PLoS One Research Article The regulation of glycerol permeability in the gastrointestinal tract is crucial to control fat deposition, lipolysis and gluconeogenesis. Knowing that the amino acid glutamine is a physiological regulator of gluconeogenesis, whereas cystine promotes adiposity, herein we investigated the effects of dietary supplementation with glutamine and cystine on the serum biochemical parameters of piglets fed on amino acid-enriched diets, as well as on the transcriptional profile of membrane water and glycerol channels aquaporins (AQPs) in the ileum portion of the small intestine and its impact on intestinal permeability. Twenty male piglets with an initial body weight of 8.8 ± 0.89 kg were allocated to four dietary treatments (n = 5) and received, during a four week-period, a basal diet without supplementation (control) or supplemented with 8 kg/ton of glutamine (Gln), cystine (Cys) or the combination of the two amino acids in equal proportions (Gln + Cys). Most biochemical parameters were found improved in piglets fed Gln and Cys diet. mRNA levels of AQP3 were found predominant over the others. Both amino acids, individually or combined, were responsible for a consistent downregulation of AQP1, AQP7 and AQP10, without impacting on water permeability. Conversely, Cys enriched diet upregulated AQP3 enhancing basolateral membranes glycerol permeability and downregulating glycerol kinase (GK) of intestinal cells. Altogether, our data reveal that amino acids dietary supplementation can modulate intestinal AQPs expression and unveil AQP3 as a promising target for adipogenesis regulation. Public Library of Science 2021-01-19 /pmc/articles/PMC7815100/ /pubmed/33465153 http://dx.doi.org/10.1371/journal.pone.0245739 Text en © 2021 Vieira da Silva et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Vieira da Silva, Inês P. Soares, Bárbara Pimpão, Catarina M. A. Pinto, Rui Costa, Teresa Freire, João P. B. Corrent, Etienne Chalvon-Demersay, Tristan Prates, José A. M. Lopes, Paula A. Soveral, Graça Glutamine and cystine-enriched diets modulate aquaporins gene expression in the small intestine of piglets |
title | Glutamine and cystine-enriched diets modulate aquaporins gene expression in the small intestine of piglets |
title_full | Glutamine and cystine-enriched diets modulate aquaporins gene expression in the small intestine of piglets |
title_fullStr | Glutamine and cystine-enriched diets modulate aquaporins gene expression in the small intestine of piglets |
title_full_unstemmed | Glutamine and cystine-enriched diets modulate aquaporins gene expression in the small intestine of piglets |
title_short | Glutamine and cystine-enriched diets modulate aquaporins gene expression in the small intestine of piglets |
title_sort | glutamine and cystine-enriched diets modulate aquaporins gene expression in the small intestine of piglets |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7815100/ https://www.ncbi.nlm.nih.gov/pubmed/33465153 http://dx.doi.org/10.1371/journal.pone.0245739 |
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