Genetic manipulation of pathogenic Leptospira: CRISPR interference (CRISPRi)-mediated gene silencing and rapid mutant recovery at 37 °C

Leptospirosis is a neglected, widespread zoonosis caused by pathogenic species of the genus Leptospira, and is responsible for 60,000 deaths per year. Pathogenic mechanisms of leptospirosis remain poorly understood mainly because targeted mutations or gene silencing in pathogenic Leptospira continue...

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Detalles Bibliográficos
Autores principales: Fernandes, L. G. V., Hornsby, R. L., Nascimento, A. L. T. O., Nally, J. E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7815788/
https://www.ncbi.nlm.nih.gov/pubmed/33469138
http://dx.doi.org/10.1038/s41598-021-81400-7

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7815788/
https://www.ncbi.nlm.nih.gov/pubmed/33469138
http://dx.doi.org/10.1038/s41598-021-81400-7

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