Cargando…

Weighted gene co-expression network analysis identifies molecular pathways and hub genes involved in broiler White Striping and Wooden Breast myopathies

In recent years, the poultry industry has experienced an increased incidence of myopathies affecting breasts of fast-growing broilers, such as White Striping (WS) and Wooden Breast (WB) defects. To explore the molecular mechanisms and genes involved in WS and WB onset, we decided to perform a Weight...

Descripción completa

Detalles Bibliográficos
Autores principales: Bordini, Martina, Zappaterra, Martina, Soglia, Francesca, Petracci, Massimiliano, Davoli, Roberta
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7815844/
https://www.ncbi.nlm.nih.gov/pubmed/33469097
http://dx.doi.org/10.1038/s41598-021-81303-7
_version_ 1783638319171633152
author Bordini, Martina
Zappaterra, Martina
Soglia, Francesca
Petracci, Massimiliano
Davoli, Roberta
author_facet Bordini, Martina
Zappaterra, Martina
Soglia, Francesca
Petracci, Massimiliano
Davoli, Roberta
author_sort Bordini, Martina
collection PubMed
description In recent years, the poultry industry has experienced an increased incidence of myopathies affecting breasts of fast-growing broilers, such as White Striping (WS) and Wooden Breast (WB) defects. To explore the molecular mechanisms and genes involved in WS and WB onset, we decided to perform a Weighted Gene Co-expression Network Analysis (WGCNA) using the gene expression profile and meat quality parameters of Pectoralis major muscles analysed in our previous study. Among the 212 modules identified by WGCNA, the red, darkred, midnightblue and paleturquoise4 modules were chosen for subsequent analysis. Functional analysis evidenced pathways involved in extracellular matrix (ECM) organization, collagen metabolism, cellular signaling and unfolded protein response. The hub gene analysis showed several genes coding for ECM components as the most interconnected nodes in the gene network (e.g. COL4A1, COL4A2, LAMA2, LAMA4, FBLN5 and FBN1). In this regard, this study suggests that alterations in ECM composition could somehow activate the cascade of biological reactions that result in the growth-related myopathies onset, and the involvement of Collagen IV alterations in activating the endoplasmic reticulum (ER) stress response may be hypothesized. Therefore, our findings provide further and innovative knowledge concerning the molecular mechanisms related to the breast abnormalities occurrence in modern broilers.
format Online
Article
Text
id pubmed-7815844
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Nature Publishing Group UK
record_format MEDLINE/PubMed
spelling pubmed-78158442021-01-21 Weighted gene co-expression network analysis identifies molecular pathways and hub genes involved in broiler White Striping and Wooden Breast myopathies Bordini, Martina Zappaterra, Martina Soglia, Francesca Petracci, Massimiliano Davoli, Roberta Sci Rep Article In recent years, the poultry industry has experienced an increased incidence of myopathies affecting breasts of fast-growing broilers, such as White Striping (WS) and Wooden Breast (WB) defects. To explore the molecular mechanisms and genes involved in WS and WB onset, we decided to perform a Weighted Gene Co-expression Network Analysis (WGCNA) using the gene expression profile and meat quality parameters of Pectoralis major muscles analysed in our previous study. Among the 212 modules identified by WGCNA, the red, darkred, midnightblue and paleturquoise4 modules were chosen for subsequent analysis. Functional analysis evidenced pathways involved in extracellular matrix (ECM) organization, collagen metabolism, cellular signaling and unfolded protein response. The hub gene analysis showed several genes coding for ECM components as the most interconnected nodes in the gene network (e.g. COL4A1, COL4A2, LAMA2, LAMA4, FBLN5 and FBN1). In this regard, this study suggests that alterations in ECM composition could somehow activate the cascade of biological reactions that result in the growth-related myopathies onset, and the involvement of Collagen IV alterations in activating the endoplasmic reticulum (ER) stress response may be hypothesized. Therefore, our findings provide further and innovative knowledge concerning the molecular mechanisms related to the breast abnormalities occurrence in modern broilers. Nature Publishing Group UK 2021-01-19 /pmc/articles/PMC7815844/ /pubmed/33469097 http://dx.doi.org/10.1038/s41598-021-81303-7 Text en © The Author(s) 2021 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Bordini, Martina
Zappaterra, Martina
Soglia, Francesca
Petracci, Massimiliano
Davoli, Roberta
Weighted gene co-expression network analysis identifies molecular pathways and hub genes involved in broiler White Striping and Wooden Breast myopathies
title Weighted gene co-expression network analysis identifies molecular pathways and hub genes involved in broiler White Striping and Wooden Breast myopathies
title_full Weighted gene co-expression network analysis identifies molecular pathways and hub genes involved in broiler White Striping and Wooden Breast myopathies
title_fullStr Weighted gene co-expression network analysis identifies molecular pathways and hub genes involved in broiler White Striping and Wooden Breast myopathies
title_full_unstemmed Weighted gene co-expression network analysis identifies molecular pathways and hub genes involved in broiler White Striping and Wooden Breast myopathies
title_short Weighted gene co-expression network analysis identifies molecular pathways and hub genes involved in broiler White Striping and Wooden Breast myopathies
title_sort weighted gene co-expression network analysis identifies molecular pathways and hub genes involved in broiler white striping and wooden breast myopathies
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7815844/
https://www.ncbi.nlm.nih.gov/pubmed/33469097
http://dx.doi.org/10.1038/s41598-021-81303-7
work_keys_str_mv AT bordinimartina weightedgenecoexpressionnetworkanalysisidentifiesmolecularpathwaysandhubgenesinvolvedinbroilerwhitestripingandwoodenbreastmyopathies
AT zappaterramartina weightedgenecoexpressionnetworkanalysisidentifiesmolecularpathwaysandhubgenesinvolvedinbroilerwhitestripingandwoodenbreastmyopathies
AT sogliafrancesca weightedgenecoexpressionnetworkanalysisidentifiesmolecularpathwaysandhubgenesinvolvedinbroilerwhitestripingandwoodenbreastmyopathies
AT petraccimassimiliano weightedgenecoexpressionnetworkanalysisidentifiesmolecularpathwaysandhubgenesinvolvedinbroilerwhitestripingandwoodenbreastmyopathies
AT davoliroberta weightedgenecoexpressionnetworkanalysisidentifiesmolecularpathwaysandhubgenesinvolvedinbroilerwhitestripingandwoodenbreastmyopathies