A novel and sensitive real-time PCR system for universal detection of poxviruses

Success in smallpox eradication was enabled by the absence of non-human reservoir for smallpox virus. However, other poxviruses with a wider host spectrum can infect humans and represent a potential health threat to humans, highlighted by a progressively increasing number of infections by (re)emergi...

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Autores principales: Luciani, Léa, Inchauste, Lucia, Ferraris, Olivier, Charrel, Rémi, Nougairède, Antoine, Piorkowski, Géraldine, Peyrefitte, Christophe, Bertagnoli, Stéphane, de Lamballerie, Xavier, Priet, Stéphane
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
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Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7815923/
https://www.ncbi.nlm.nih.gov/pubmed/33469067
http://dx.doi.org/10.1038/s41598-021-81376-4
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author Luciani, Léa
Inchauste, Lucia
Ferraris, Olivier
Charrel, Rémi
Nougairède, Antoine
Piorkowski, Géraldine
Peyrefitte, Christophe
Bertagnoli, Stéphane
de Lamballerie, Xavier
Priet, Stéphane
author_facet Luciani, Léa
Inchauste, Lucia
Ferraris, Olivier
Charrel, Rémi
Nougairède, Antoine
Piorkowski, Géraldine
Peyrefitte, Christophe
Bertagnoli, Stéphane
de Lamballerie, Xavier
Priet, Stéphane
author_sort Luciani, Léa
collection PubMed
description Success in smallpox eradication was enabled by the absence of non-human reservoir for smallpox virus. However, other poxviruses with a wider host spectrum can infect humans and represent a potential health threat to humans, highlighted by a progressively increasing number of infections by (re)emerging poxviruses, requiring new improved diagnostic and epidemiological tools. We describe here a real-time PCR assay targeting a highly conserved region of the poxvirus genome, thus allowing a pan-Poxvirus detection (Chordopoxvirinae and Entomopoxvirinae). This system is specific (99.8% for vertebrate samples and 99.7% for arthropods samples), sensitive (100% for vertebrate samples and 86.3% for arthropods samples) and presents low limit of detection (< 1000 DNA copies/reaction). In addition, this system could be also valuable for virus discovery and epidemiological projects.
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spelling pubmed-78159232021-01-21 A novel and sensitive real-time PCR system for universal detection of poxviruses Luciani, Léa Inchauste, Lucia Ferraris, Olivier Charrel, Rémi Nougairède, Antoine Piorkowski, Géraldine Peyrefitte, Christophe Bertagnoli, Stéphane de Lamballerie, Xavier Priet, Stéphane Sci Rep Article Success in smallpox eradication was enabled by the absence of non-human reservoir for smallpox virus. However, other poxviruses with a wider host spectrum can infect humans and represent a potential health threat to humans, highlighted by a progressively increasing number of infections by (re)emerging poxviruses, requiring new improved diagnostic and epidemiological tools. We describe here a real-time PCR assay targeting a highly conserved region of the poxvirus genome, thus allowing a pan-Poxvirus detection (Chordopoxvirinae and Entomopoxvirinae). This system is specific (99.8% for vertebrate samples and 99.7% for arthropods samples), sensitive (100% for vertebrate samples and 86.3% for arthropods samples) and presents low limit of detection (< 1000 DNA copies/reaction). In addition, this system could be also valuable for virus discovery and epidemiological projects. Nature Publishing Group UK 2021-01-19 /pmc/articles/PMC7815923/ /pubmed/33469067 http://dx.doi.org/10.1038/s41598-021-81376-4 Text en © The Author(s) 2021, corrected publication 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Luciani, Léa
Inchauste, Lucia
Ferraris, Olivier
Charrel, Rémi
Nougairède, Antoine
Piorkowski, Géraldine
Peyrefitte, Christophe
Bertagnoli, Stéphane
de Lamballerie, Xavier
Priet, Stéphane
A novel and sensitive real-time PCR system for universal detection of poxviruses
title A novel and sensitive real-time PCR system for universal detection of poxviruses
title_full A novel and sensitive real-time PCR system for universal detection of poxviruses
title_fullStr A novel and sensitive real-time PCR system for universal detection of poxviruses
title_full_unstemmed A novel and sensitive real-time PCR system for universal detection of poxviruses
title_short A novel and sensitive real-time PCR system for universal detection of poxviruses
title_sort novel and sensitive real-time pcr system for universal detection of poxviruses
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7815923/
https://www.ncbi.nlm.nih.gov/pubmed/33469067
http://dx.doi.org/10.1038/s41598-021-81376-4
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