Cargando…

A mobile microvolume UV/visible light spectrophotometer for the measurement of levofloxacin in saliva

INTRODUCTION: Therapeutic drug monitoring (TDM) for personalized dosing of fluoroquinolones has been recommended to optimize efficacy and reduce acquired drug resistance in the treatment of MDR TB. Therefore, the aim of this study was to develop a simple, low-cost, robust assay for TDM using mobile...

Descripción completa

Detalles Bibliográficos
Autores principales: Alffenaar, Jan-Willem C, Jongedijk, Erwin M, van Winkel, Claudia A J, Sariko, Margaretha, Heysell, Scott K, Mpagama, Stellah, Touw, Daan J
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7816168/
https://www.ncbi.nlm.nih.gov/pubmed/33089322
http://dx.doi.org/10.1093/jac/dkaa420
_version_ 1783638386504892416
author Alffenaar, Jan-Willem C
Jongedijk, Erwin M
van Winkel, Claudia A J
Sariko, Margaretha
Heysell, Scott K
Mpagama, Stellah
Touw, Daan J
author_facet Alffenaar, Jan-Willem C
Jongedijk, Erwin M
van Winkel, Claudia A J
Sariko, Margaretha
Heysell, Scott K
Mpagama, Stellah
Touw, Daan J
author_sort Alffenaar, Jan-Willem C
collection PubMed
description INTRODUCTION: Therapeutic drug monitoring (TDM) for personalized dosing of fluoroquinolones has been recommended to optimize efficacy and reduce acquired drug resistance in the treatment of MDR TB. Therefore, the aim of this study was to develop a simple, low-cost, robust assay for TDM using mobile UV/visible light (UV/VIS) spectrophotometry to quantify levofloxacin in human saliva at the point of care for TB endemic settings. METHODS: All experiments were performed on a mobile UV/VIS spectrophotometer. The levofloxacin concentration was quantified by using the amplitude of the second-order spectrum between 300 and 400 nm of seven calibrators. The concentration of spiked samples was calculated from the spectrum amplitude using linear regression. The method was validated for selectivity, specificity, linearity, accuracy and precision. Drugs frequently co-administered were tested for interference. RESULTS: The calibration curve was linear over a range of 2.5–50.0 mg/L for levofloxacin, with a correlation coefficient of 0.997. Calculated accuracy ranged from –5.2% to 2.4%. Overall precision ranged from 2.1% to 16.1%. Application of the Savitsky–Golay method reduced the effect of interferents on the quantitation of levofloxacin. Although rifampicin and pyrazinamide showed analytical interference at the lower limit of quantitation of levofloxacin concentrations, this interference had no implication on decisions regarding the levofloxacin dose. CONCLUSIONS: A simple UV/VIS spectrophotometric method to quantify levofloxacin in saliva using a mobile nanophotometer has been validated. This method can be evaluated in programmatic settings to identify patients with low levofloxacin drug exposure to trigger personalized dose adjustment.
format Online
Article
Text
id pubmed-7816168
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher Oxford University Press
record_format MEDLINE/PubMed
spelling pubmed-78161682021-01-26 A mobile microvolume UV/visible light spectrophotometer for the measurement of levofloxacin in saliva Alffenaar, Jan-Willem C Jongedijk, Erwin M van Winkel, Claudia A J Sariko, Margaretha Heysell, Scott K Mpagama, Stellah Touw, Daan J J Antimicrob Chemother Original Research INTRODUCTION: Therapeutic drug monitoring (TDM) for personalized dosing of fluoroquinolones has been recommended to optimize efficacy and reduce acquired drug resistance in the treatment of MDR TB. Therefore, the aim of this study was to develop a simple, low-cost, robust assay for TDM using mobile UV/visible light (UV/VIS) spectrophotometry to quantify levofloxacin in human saliva at the point of care for TB endemic settings. METHODS: All experiments were performed on a mobile UV/VIS spectrophotometer. The levofloxacin concentration was quantified by using the amplitude of the second-order spectrum between 300 and 400 nm of seven calibrators. The concentration of spiked samples was calculated from the spectrum amplitude using linear regression. The method was validated for selectivity, specificity, linearity, accuracy and precision. Drugs frequently co-administered were tested for interference. RESULTS: The calibration curve was linear over a range of 2.5–50.0 mg/L for levofloxacin, with a correlation coefficient of 0.997. Calculated accuracy ranged from –5.2% to 2.4%. Overall precision ranged from 2.1% to 16.1%. Application of the Savitsky–Golay method reduced the effect of interferents on the quantitation of levofloxacin. Although rifampicin and pyrazinamide showed analytical interference at the lower limit of quantitation of levofloxacin concentrations, this interference had no implication on decisions regarding the levofloxacin dose. CONCLUSIONS: A simple UV/VIS spectrophotometric method to quantify levofloxacin in saliva using a mobile nanophotometer has been validated. This method can be evaluated in programmatic settings to identify patients with low levofloxacin drug exposure to trigger personalized dose adjustment. Oxford University Press 2020-10-22 /pmc/articles/PMC7816168/ /pubmed/33089322 http://dx.doi.org/10.1093/jac/dkaa420 Text en © The Author(s) 2020. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Research
Alffenaar, Jan-Willem C
Jongedijk, Erwin M
van Winkel, Claudia A J
Sariko, Margaretha
Heysell, Scott K
Mpagama, Stellah
Touw, Daan J
A mobile microvolume UV/visible light spectrophotometer for the measurement of levofloxacin in saliva
title A mobile microvolume UV/visible light spectrophotometer for the measurement of levofloxacin in saliva
title_full A mobile microvolume UV/visible light spectrophotometer for the measurement of levofloxacin in saliva
title_fullStr A mobile microvolume UV/visible light spectrophotometer for the measurement of levofloxacin in saliva
title_full_unstemmed A mobile microvolume UV/visible light spectrophotometer for the measurement of levofloxacin in saliva
title_short A mobile microvolume UV/visible light spectrophotometer for the measurement of levofloxacin in saliva
title_sort mobile microvolume uv/visible light spectrophotometer for the measurement of levofloxacin in saliva
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7816168/
https://www.ncbi.nlm.nih.gov/pubmed/33089322
http://dx.doi.org/10.1093/jac/dkaa420
work_keys_str_mv AT alffenaarjanwillemc amobilemicrovolumeuvvisiblelightspectrophotometerforthemeasurementoflevofloxacininsaliva
AT jongedijkerwinm amobilemicrovolumeuvvisiblelightspectrophotometerforthemeasurementoflevofloxacininsaliva
AT vanwinkelclaudiaaj amobilemicrovolumeuvvisiblelightspectrophotometerforthemeasurementoflevofloxacininsaliva
AT sarikomargaretha amobilemicrovolumeuvvisiblelightspectrophotometerforthemeasurementoflevofloxacininsaliva
AT heysellscottk amobilemicrovolumeuvvisiblelightspectrophotometerforthemeasurementoflevofloxacininsaliva
AT mpagamastellah amobilemicrovolumeuvvisiblelightspectrophotometerforthemeasurementoflevofloxacininsaliva
AT touwdaanj amobilemicrovolumeuvvisiblelightspectrophotometerforthemeasurementoflevofloxacininsaliva
AT alffenaarjanwillemc mobilemicrovolumeuvvisiblelightspectrophotometerforthemeasurementoflevofloxacininsaliva
AT jongedijkerwinm mobilemicrovolumeuvvisiblelightspectrophotometerforthemeasurementoflevofloxacininsaliva
AT vanwinkelclaudiaaj mobilemicrovolumeuvvisiblelightspectrophotometerforthemeasurementoflevofloxacininsaliva
AT sarikomargaretha mobilemicrovolumeuvvisiblelightspectrophotometerforthemeasurementoflevofloxacininsaliva
AT heysellscottk mobilemicrovolumeuvvisiblelightspectrophotometerforthemeasurementoflevofloxacininsaliva
AT mpagamastellah mobilemicrovolumeuvvisiblelightspectrophotometerforthemeasurementoflevofloxacininsaliva
AT touwdaanj mobilemicrovolumeuvvisiblelightspectrophotometerforthemeasurementoflevofloxacininsaliva