Genetic diversity of Giardia isolates from patients in Chandigarh region: India

OBJECTIVE: The aim of study was to characterize Giardia isolates genetically among patients in Chandigarh region, India. For this, nested PCR targeting fragment of the glutamate dehydrogenase (GLUD1 earlier named as GDH) gene was used. Phylogenetic analysis was done by constructing neighbor-joining...

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Autores principales: Thakur, Shabnam, Kaur, Upninder, Sehgal, Rakesh
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2021
Materias:
Research Note
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7816347/
https://www.ncbi.nlm.nih.gov/pubmed/33468229
http://dx.doi.org/10.1186/s13104-020-05419-1
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author Thakur, Shabnam
Kaur, Upninder
Sehgal, Rakesh
author_facet Thakur, Shabnam
Kaur, Upninder
Sehgal, Rakesh
author_sort Thakur, Shabnam
collection PubMed
description OBJECTIVE: The aim of study was to characterize Giardia isolates genetically among patients in Chandigarh region, India. For this, nested PCR targeting fragment of the glutamate dehydrogenase (GLUD1 earlier named as GDH) gene was used. Phylogenetic analysis was done by constructing neighbor-joining tree made out of the nucleotide sequences of G. intestinalis isolates obtained in this study and with the known sequences published in GenBank. RESULTS: Out of 40 samples, GLUD1 gene was amplified in 33 samples (82.5%). The product of GLUD1 gene was successfully sequenced only in 32 samples. In these samples, assemblage B was found in 27 (84.37%) samples whereas 5 (15.6%) samples had assemblage A. Among assemblage B most of them were of BIII. Therefore, genotyping of Giardia would be helpful in conducting epidemiological studies.
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spelling pubmed-78163472021-01-21 Genetic diversity of Giardia isolates from patients in Chandigarh region: India Thakur, Shabnam Kaur, Upninder Sehgal, Rakesh BMC Res Notes Research Note OBJECTIVE: The aim of study was to characterize Giardia isolates genetically among patients in Chandigarh region, India. For this, nested PCR targeting fragment of the glutamate dehydrogenase (GLUD1 earlier named as GDH) gene was used. Phylogenetic analysis was done by constructing neighbor-joining tree made out of the nucleotide sequences of G. intestinalis isolates obtained in this study and with the known sequences published in GenBank. RESULTS: Out of 40 samples, GLUD1 gene was amplified in 33 samples (82.5%). The product of GLUD1 gene was successfully sequenced only in 32 samples. In these samples, assemblage B was found in 27 (84.37%) samples whereas 5 (15.6%) samples had assemblage A. Among assemblage B most of them were of BIII. Therefore, genotyping of Giardia would be helpful in conducting epidemiological studies. BioMed Central 2021-01-19 /pmc/articles/PMC7816347/ /pubmed/33468229 http://dx.doi.org/10.1186/s13104-020-05419-1 Text en © The Author(s) 2021 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research Note
Thakur, Shabnam
Kaur, Upninder
Sehgal, Rakesh
Genetic diversity of Giardia isolates from patients in Chandigarh region: India
title Genetic diversity of Giardia isolates from patients in Chandigarh region: India
title_full Genetic diversity of Giardia isolates from patients in Chandigarh region: India
title_fullStr Genetic diversity of Giardia isolates from patients in Chandigarh region: India
title_full_unstemmed Genetic diversity of Giardia isolates from patients in Chandigarh region: India
title_short Genetic diversity of Giardia isolates from patients in Chandigarh region: India
title_sort genetic diversity of giardia isolates from patients in chandigarh region: india
topic Research Note
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7816347/
https://www.ncbi.nlm.nih.gov/pubmed/33468229
http://dx.doi.org/10.1186/s13104-020-05419-1
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