Exogenous application of xanthine and uric acid and nucleobase-ascorbate transporter MdNAT7 expression regulate salinity tolerance in apple

BACKGROUND: Soil salinity is a critical threat to global agriculture. In plants, the accumulation of xanthine activates xanthine dehydrogenase (XDH), which catalyses the oxidation/conversion of xanthine to uric acid to remove excess reactive oxygen species (ROS). The nucleobase-ascorbate transporter...

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Autores principales: Sun, Tingting, Pei, Tingting, Yang, Lulu, Zhang, Zhijun, Li, Mingjun, Liu, Yuerong, Ma, Fengwang, Liu, Changhai
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2021
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7816448/
https://www.ncbi.nlm.nih.gov/pubmed/33468049
http://dx.doi.org/10.1186/s12870-021-02831-y
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author Sun, Tingting
Pei, Tingting
Yang, Lulu
Zhang, Zhijun
Li, Mingjun
Liu, Yuerong
Ma, Fengwang
Liu, Changhai
author_facet Sun, Tingting
Pei, Tingting
Yang, Lulu
Zhang, Zhijun
Li, Mingjun
Liu, Yuerong
Ma, Fengwang
Liu, Changhai
author_sort Sun, Tingting
collection PubMed
description BACKGROUND: Soil salinity is a critical threat to global agriculture. In plants, the accumulation of xanthine activates xanthine dehydrogenase (XDH), which catalyses the oxidation/conversion of xanthine to uric acid to remove excess reactive oxygen species (ROS). The nucleobase-ascorbate transporter (NAT) family is also known as the nucleobase-cation symporter (NCS) or AzgA-like family. NAT is known to transport xanthine and uric acid in plants. The expression of MdNAT is influenced by salinity stress in apple. RESULTS: In this study, we discovered that exogenous application of xanthine and uric acid enhanced the resistance of apple plants to salinity stress. In addition, MdNAT7 overexpression transgenic apple plants showed enhanced xanthine and uric acid concentrations and improved tolerance to salinity stress compared with nontransgenic plants, while opposite phenotypes were observed for MdNAT7 RNAi plants. These differences were probably due to the enhancement or impairment of ROS scavenging and ion homeostasis abilities. CONCLUSION: Our results demonstrate that xanthine and uric acid have potential uses in salt stress alleviation, and MdNAT7 can be utilized as a candidate gene to engineer resistance to salt stress in plants. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12870-021-02831-y.
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spelling pubmed-78164482021-01-22 Exogenous application of xanthine and uric acid and nucleobase-ascorbate transporter MdNAT7 expression regulate salinity tolerance in apple Sun, Tingting Pei, Tingting Yang, Lulu Zhang, Zhijun Li, Mingjun Liu, Yuerong Ma, Fengwang Liu, Changhai BMC Plant Biol Research Article BACKGROUND: Soil salinity is a critical threat to global agriculture. In plants, the accumulation of xanthine activates xanthine dehydrogenase (XDH), which catalyses the oxidation/conversion of xanthine to uric acid to remove excess reactive oxygen species (ROS). The nucleobase-ascorbate transporter (NAT) family is also known as the nucleobase-cation symporter (NCS) or AzgA-like family. NAT is known to transport xanthine and uric acid in plants. The expression of MdNAT is influenced by salinity stress in apple. RESULTS: In this study, we discovered that exogenous application of xanthine and uric acid enhanced the resistance of apple plants to salinity stress. In addition, MdNAT7 overexpression transgenic apple plants showed enhanced xanthine and uric acid concentrations and improved tolerance to salinity stress compared with nontransgenic plants, while opposite phenotypes were observed for MdNAT7 RNAi plants. These differences were probably due to the enhancement or impairment of ROS scavenging and ion homeostasis abilities. CONCLUSION: Our results demonstrate that xanthine and uric acid have potential uses in salt stress alleviation, and MdNAT7 can be utilized as a candidate gene to engineer resistance to salt stress in plants. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12870-021-02831-y. BioMed Central 2021-01-19 /pmc/articles/PMC7816448/ /pubmed/33468049 http://dx.doi.org/10.1186/s12870-021-02831-y Text en © The Author(s) 2021 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research Article
Sun, Tingting
Pei, Tingting
Yang, Lulu
Zhang, Zhijun
Li, Mingjun
Liu, Yuerong
Ma, Fengwang
Liu, Changhai
Exogenous application of xanthine and uric acid and nucleobase-ascorbate transporter MdNAT7 expression regulate salinity tolerance in apple
title Exogenous application of xanthine and uric acid and nucleobase-ascorbate transporter MdNAT7 expression regulate salinity tolerance in apple
title_full Exogenous application of xanthine and uric acid and nucleobase-ascorbate transporter MdNAT7 expression regulate salinity tolerance in apple
title_fullStr Exogenous application of xanthine and uric acid and nucleobase-ascorbate transporter MdNAT7 expression regulate salinity tolerance in apple
title_full_unstemmed Exogenous application of xanthine and uric acid and nucleobase-ascorbate transporter MdNAT7 expression regulate salinity tolerance in apple
title_short Exogenous application of xanthine and uric acid and nucleobase-ascorbate transporter MdNAT7 expression regulate salinity tolerance in apple
title_sort exogenous application of xanthine and uric acid and nucleobase-ascorbate transporter mdnat7 expression regulate salinity tolerance in apple
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7816448/
https://www.ncbi.nlm.nih.gov/pubmed/33468049
http://dx.doi.org/10.1186/s12870-021-02831-y
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