Cargando…

Recombinant SARS-CoV-2 RBD with a built in T helper epitope induces strong neutralization antibody response

Without approved vaccines and specific treatments, COVID-19 is spreading around the world with above 26 million cases and approximately 864 thousand deaths until now. An efficacious and affordable vaccine is urgently needed. The Val308 – Gly548 of spike protein of SARS-CoV-2 linked with Gln830 – Glu...

Descripción completa

Detalles Bibliográficos
Autores principales: Su, Qiu-Dong, Zou, Ye-Ning, Yi, Yao, Shen, Li-Ping, Ye, Xiang-Zhong, Zhang, Yang, Wang, Hui, Ke, Hong, Song, Jing-Dong, Hu, Ke-Ping, Cheng, Bo-Lin, Qiu, Feng, Yu, Peng-Cheng, Zhou, Wen-Ting, Zhao, Ran, Cao, Lei, Dong, Gao-Feng, Bi, Sheng-Li, Wu, Gui-Zhen, Gao, George Fu, Zheng, Jerry
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier Ltd. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7816590/
https://www.ncbi.nlm.nih.gov/pubmed/33516600
http://dx.doi.org/10.1016/j.vaccine.2021.01.044
_version_ 1783638474453155840
author Su, Qiu-Dong
Zou, Ye-Ning
Yi, Yao
Shen, Li-Ping
Ye, Xiang-Zhong
Zhang, Yang
Wang, Hui
Ke, Hong
Song, Jing-Dong
Hu, Ke-Ping
Cheng, Bo-Lin
Qiu, Feng
Yu, Peng-Cheng
Zhou, Wen-Ting
Zhao, Ran
Cao, Lei
Dong, Gao-Feng
Bi, Sheng-Li
Wu, Gui-Zhen
Gao, George Fu
Zheng, Jerry
author_facet Su, Qiu-Dong
Zou, Ye-Ning
Yi, Yao
Shen, Li-Ping
Ye, Xiang-Zhong
Zhang, Yang
Wang, Hui
Ke, Hong
Song, Jing-Dong
Hu, Ke-Ping
Cheng, Bo-Lin
Qiu, Feng
Yu, Peng-Cheng
Zhou, Wen-Ting
Zhao, Ran
Cao, Lei
Dong, Gao-Feng
Bi, Sheng-Li
Wu, Gui-Zhen
Gao, George Fu
Zheng, Jerry
author_sort Su, Qiu-Dong
collection PubMed
description Without approved vaccines and specific treatments, COVID-19 is spreading around the world with above 26 million cases and approximately 864 thousand deaths until now. An efficacious and affordable vaccine is urgently needed. The Val308 – Gly548 of spike protein of SARS-CoV-2 linked with Gln830 – Glu843 of Tetanus toxoid (TT peptide) (designated as S1-4) and without TT peptide (designated as S1-5) were expressed and renatured. The antigenicity and immunogenicity of S1-4 were evaluated by Western Blotting (WB) in vitro and immune responses in mice, respectively. The protective efficiency was measured preliminarily by microneutralization assay (MN50). The soluble S1-4 and S1-5 protein was prepared to high homogeneity and purity. Adjuvanted with Alum, S1-4 protein stimulated a strong antibody response in immunized mice and caused a major Th2-type cellular immunity supplemented with Th1-type immunity. Furthermore, the immunized sera could protect the Vero E6 cells from SARS-CoV-2 infection with neutralizing antibody titer 256. Recombinant SARS-CoV-2 RBD with a built in T helper epitope could stimulate both strong humoral immunity supplemented with cellular immunity in mice, demonstrating that it could be a promising subunit vaccine candidate.
format Online
Article
Text
id pubmed-7816590
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Elsevier Ltd.
record_format MEDLINE/PubMed
spelling pubmed-78165902021-01-21 Recombinant SARS-CoV-2 RBD with a built in T helper epitope induces strong neutralization antibody response Su, Qiu-Dong Zou, Ye-Ning Yi, Yao Shen, Li-Ping Ye, Xiang-Zhong Zhang, Yang Wang, Hui Ke, Hong Song, Jing-Dong Hu, Ke-Ping Cheng, Bo-Lin Qiu, Feng Yu, Peng-Cheng Zhou, Wen-Ting Zhao, Ran Cao, Lei Dong, Gao-Feng Bi, Sheng-Li Wu, Gui-Zhen Gao, George Fu Zheng, Jerry Vaccine Article Without approved vaccines and specific treatments, COVID-19 is spreading around the world with above 26 million cases and approximately 864 thousand deaths until now. An efficacious and affordable vaccine is urgently needed. The Val308 – Gly548 of spike protein of SARS-CoV-2 linked with Gln830 – Glu843 of Tetanus toxoid (TT peptide) (designated as S1-4) and without TT peptide (designated as S1-5) were expressed and renatured. The antigenicity and immunogenicity of S1-4 were evaluated by Western Blotting (WB) in vitro and immune responses in mice, respectively. The protective efficiency was measured preliminarily by microneutralization assay (MN50). The soluble S1-4 and S1-5 protein was prepared to high homogeneity and purity. Adjuvanted with Alum, S1-4 protein stimulated a strong antibody response in immunized mice and caused a major Th2-type cellular immunity supplemented with Th1-type immunity. Furthermore, the immunized sera could protect the Vero E6 cells from SARS-CoV-2 infection with neutralizing antibody titer 256. Recombinant SARS-CoV-2 RBD with a built in T helper epitope could stimulate both strong humoral immunity supplemented with cellular immunity in mice, demonstrating that it could be a promising subunit vaccine candidate. Elsevier Ltd. 2021-02-22 2021-01-20 /pmc/articles/PMC7816590/ /pubmed/33516600 http://dx.doi.org/10.1016/j.vaccine.2021.01.044 Text en © 2021 Elsevier Ltd. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Su, Qiu-Dong
Zou, Ye-Ning
Yi, Yao
Shen, Li-Ping
Ye, Xiang-Zhong
Zhang, Yang
Wang, Hui
Ke, Hong
Song, Jing-Dong
Hu, Ke-Ping
Cheng, Bo-Lin
Qiu, Feng
Yu, Peng-Cheng
Zhou, Wen-Ting
Zhao, Ran
Cao, Lei
Dong, Gao-Feng
Bi, Sheng-Li
Wu, Gui-Zhen
Gao, George Fu
Zheng, Jerry
Recombinant SARS-CoV-2 RBD with a built in T helper epitope induces strong neutralization antibody response
title Recombinant SARS-CoV-2 RBD with a built in T helper epitope induces strong neutralization antibody response
title_full Recombinant SARS-CoV-2 RBD with a built in T helper epitope induces strong neutralization antibody response
title_fullStr Recombinant SARS-CoV-2 RBD with a built in T helper epitope induces strong neutralization antibody response
title_full_unstemmed Recombinant SARS-CoV-2 RBD with a built in T helper epitope induces strong neutralization antibody response
title_short Recombinant SARS-CoV-2 RBD with a built in T helper epitope induces strong neutralization antibody response
title_sort recombinant sars-cov-2 rbd with a built in t helper epitope induces strong neutralization antibody response
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7816590/
https://www.ncbi.nlm.nih.gov/pubmed/33516600
http://dx.doi.org/10.1016/j.vaccine.2021.01.044
work_keys_str_mv AT suqiudong recombinantsarscov2rbdwithabuiltinthelperepitopeinducesstrongneutralizationantibodyresponse
AT zouyening recombinantsarscov2rbdwithabuiltinthelperepitopeinducesstrongneutralizationantibodyresponse
AT yiyao recombinantsarscov2rbdwithabuiltinthelperepitopeinducesstrongneutralizationantibodyresponse
AT shenliping recombinantsarscov2rbdwithabuiltinthelperepitopeinducesstrongneutralizationantibodyresponse
AT yexiangzhong recombinantsarscov2rbdwithabuiltinthelperepitopeinducesstrongneutralizationantibodyresponse
AT zhangyang recombinantsarscov2rbdwithabuiltinthelperepitopeinducesstrongneutralizationantibodyresponse
AT wanghui recombinantsarscov2rbdwithabuiltinthelperepitopeinducesstrongneutralizationantibodyresponse
AT kehong recombinantsarscov2rbdwithabuiltinthelperepitopeinducesstrongneutralizationantibodyresponse
AT songjingdong recombinantsarscov2rbdwithabuiltinthelperepitopeinducesstrongneutralizationantibodyresponse
AT hukeping recombinantsarscov2rbdwithabuiltinthelperepitopeinducesstrongneutralizationantibodyresponse
AT chengbolin recombinantsarscov2rbdwithabuiltinthelperepitopeinducesstrongneutralizationantibodyresponse
AT qiufeng recombinantsarscov2rbdwithabuiltinthelperepitopeinducesstrongneutralizationantibodyresponse
AT yupengcheng recombinantsarscov2rbdwithabuiltinthelperepitopeinducesstrongneutralizationantibodyresponse
AT zhouwenting recombinantsarscov2rbdwithabuiltinthelperepitopeinducesstrongneutralizationantibodyresponse
AT zhaoran recombinantsarscov2rbdwithabuiltinthelperepitopeinducesstrongneutralizationantibodyresponse
AT caolei recombinantsarscov2rbdwithabuiltinthelperepitopeinducesstrongneutralizationantibodyresponse
AT donggaofeng recombinantsarscov2rbdwithabuiltinthelperepitopeinducesstrongneutralizationantibodyresponse
AT bishengli recombinantsarscov2rbdwithabuiltinthelperepitopeinducesstrongneutralizationantibodyresponse
AT wuguizhen recombinantsarscov2rbdwithabuiltinthelperepitopeinducesstrongneutralizationantibodyresponse
AT gaogeorgefu recombinantsarscov2rbdwithabuiltinthelperepitopeinducesstrongneutralizationantibodyresponse
AT zhengjerry recombinantsarscov2rbdwithabuiltinthelperepitopeinducesstrongneutralizationantibodyresponse