Cargando…
Method comparison studies of telomere length measurement using qPCR approaches: A critical appraisal of the literature
Use of telomere length (TL) as a biomarker for various environmental exposures and diseases has increased in recent years. Various methods have been developed to measure telomere length. Polymerase chain reaction (PCR)-based methods remain wide-spread for population-based studies due to the high-thr...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7817045/ https://www.ncbi.nlm.nih.gov/pubmed/33471860 http://dx.doi.org/10.1371/journal.pone.0245582 |
_version_ | 1783638562997010432 |
---|---|
author | Lindrose, Alyssa R. McLester-Davis, Lauren W. Y. Tristano, Renee I. Kataria, Leila Gadalla, Shahinaz M. Eisenberg, Dan T. A. Verhulst, Simon Drury, Stacy |
author_facet | Lindrose, Alyssa R. McLester-Davis, Lauren W. Y. Tristano, Renee I. Kataria, Leila Gadalla, Shahinaz M. Eisenberg, Dan T. A. Verhulst, Simon Drury, Stacy |
author_sort | Lindrose, Alyssa R. |
collection | PubMed |
description | Use of telomere length (TL) as a biomarker for various environmental exposures and diseases has increased in recent years. Various methods have been developed to measure telomere length. Polymerase chain reaction (PCR)-based methods remain wide-spread for population-based studies due to the high-throughput capability. While several studies have evaluated the repeatability and reproducibility of different TL measurement methods, the results have been variable. We conducted a literature review of TL measurement cross-method comparison studies that included a PCR-based method published between January 1, 2002 and May 25, 2020. A total of 25 articles were found that matched the inclusion criteria. Papers were reviewed for quality of methodologic reporting of sample and DNA quality, PCR assay characteristics, sample blinding, and analytic approaches to determine final TL. Overall, methodologic reporting was low as assessed by two different reporting guidelines for qPCR-based TL measurement. There was a wide range in the reported correlation between methods (as assessed by Pearson’s r) and few studies utilized the recommended intra-class correlation coefficient (ICC) for assessment of assay repeatability and methodologic comparisons. The sample size for nearly all studies was less than 100, raising concerns about statistical power. Overall, this review found that the current literature on the relation between TL measurement methods is lacking in validity and scientific rigor. In light of these findings, we present reporting guidelines for PCR-based TL measurement methods and results of analyses of the effect of assay repeatability (ICC) on statistical power of cross-sectional and longitudinal studies. Additional cross-laboratory studies with rigorous methodologic and statistical reporting, adequate sample size, and blinding are essential to accurately determine assay repeatability and replicability as well as the relation between TL measurement methods. |
format | Online Article Text |
id | pubmed-7817045 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-78170452021-01-28 Method comparison studies of telomere length measurement using qPCR approaches: A critical appraisal of the literature Lindrose, Alyssa R. McLester-Davis, Lauren W. Y. Tristano, Renee I. Kataria, Leila Gadalla, Shahinaz M. Eisenberg, Dan T. A. Verhulst, Simon Drury, Stacy PLoS One Research Article Use of telomere length (TL) as a biomarker for various environmental exposures and diseases has increased in recent years. Various methods have been developed to measure telomere length. Polymerase chain reaction (PCR)-based methods remain wide-spread for population-based studies due to the high-throughput capability. While several studies have evaluated the repeatability and reproducibility of different TL measurement methods, the results have been variable. We conducted a literature review of TL measurement cross-method comparison studies that included a PCR-based method published between January 1, 2002 and May 25, 2020. A total of 25 articles were found that matched the inclusion criteria. Papers were reviewed for quality of methodologic reporting of sample and DNA quality, PCR assay characteristics, sample blinding, and analytic approaches to determine final TL. Overall, methodologic reporting was low as assessed by two different reporting guidelines for qPCR-based TL measurement. There was a wide range in the reported correlation between methods (as assessed by Pearson’s r) and few studies utilized the recommended intra-class correlation coefficient (ICC) for assessment of assay repeatability and methodologic comparisons. The sample size for nearly all studies was less than 100, raising concerns about statistical power. Overall, this review found that the current literature on the relation between TL measurement methods is lacking in validity and scientific rigor. In light of these findings, we present reporting guidelines for PCR-based TL measurement methods and results of analyses of the effect of assay repeatability (ICC) on statistical power of cross-sectional and longitudinal studies. Additional cross-laboratory studies with rigorous methodologic and statistical reporting, adequate sample size, and blinding are essential to accurately determine assay repeatability and replicability as well as the relation between TL measurement methods. Public Library of Science 2021-01-20 /pmc/articles/PMC7817045/ /pubmed/33471860 http://dx.doi.org/10.1371/journal.pone.0245582 Text en © 2021 Lindrose et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Lindrose, Alyssa R. McLester-Davis, Lauren W. Y. Tristano, Renee I. Kataria, Leila Gadalla, Shahinaz M. Eisenberg, Dan T. A. Verhulst, Simon Drury, Stacy Method comparison studies of telomere length measurement using qPCR approaches: A critical appraisal of the literature |
title | Method comparison studies of telomere length measurement using qPCR approaches: A critical appraisal of the literature |
title_full | Method comparison studies of telomere length measurement using qPCR approaches: A critical appraisal of the literature |
title_fullStr | Method comparison studies of telomere length measurement using qPCR approaches: A critical appraisal of the literature |
title_full_unstemmed | Method comparison studies of telomere length measurement using qPCR approaches: A critical appraisal of the literature |
title_short | Method comparison studies of telomere length measurement using qPCR approaches: A critical appraisal of the literature |
title_sort | method comparison studies of telomere length measurement using qpcr approaches: a critical appraisal of the literature |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7817045/ https://www.ncbi.nlm.nih.gov/pubmed/33471860 http://dx.doi.org/10.1371/journal.pone.0245582 |
work_keys_str_mv | AT lindrosealyssar methodcomparisonstudiesoftelomerelengthmeasurementusingqpcrapproachesacriticalappraisaloftheliterature AT mclesterdavislaurenwy methodcomparisonstudiesoftelomerelengthmeasurementusingqpcrapproachesacriticalappraisaloftheliterature AT tristanoreneei methodcomparisonstudiesoftelomerelengthmeasurementusingqpcrapproachesacriticalappraisaloftheliterature AT katarialeila methodcomparisonstudiesoftelomerelengthmeasurementusingqpcrapproachesacriticalappraisaloftheliterature AT gadallashahinazm methodcomparisonstudiesoftelomerelengthmeasurementusingqpcrapproachesacriticalappraisaloftheliterature AT eisenbergdanta methodcomparisonstudiesoftelomerelengthmeasurementusingqpcrapproachesacriticalappraisaloftheliterature AT verhulstsimon methodcomparisonstudiesoftelomerelengthmeasurementusingqpcrapproachesacriticalappraisaloftheliterature AT drurystacy methodcomparisonstudiesoftelomerelengthmeasurementusingqpcrapproachesacriticalappraisaloftheliterature |