Mitochondria-specific delivery system for targeted regulation of mitochondrial gene expression

Targeted regulation of mitochondrial gene expression is challenging due to the lack of a mitochondria-specific delivery system. We have previously developed various stimuli-responsive nanoparticle (NP)-based delivery systems to transport nucleic acids for regulation of target gene expression. This p...

Descripción completa

Detalles Bibliográficos
Autores principales: Su, Shicheng, Cao, Shuwen, Liu, Jiayu, Xu, Xiaoding
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7817499/
https://www.ncbi.nlm.nih.gov/pubmed/33511358
http://dx.doi.org/10.1016/j.xpro.2020.100275
_version_ 1783638652261236736
author Su, Shicheng
Cao, Shuwen
Liu, Jiayu
Xu, Xiaoding
author_facet Su, Shicheng
Cao, Shuwen
Liu, Jiayu
Xu, Xiaoding
author_sort Su, Shicheng
collection PubMed
description Targeted regulation of mitochondrial gene expression is challenging due to the lack of a mitochondria-specific delivery system. We have previously developed various stimuli-responsive nanoparticle (NP)-based delivery systems to transport nucleic acids for regulation of target gene expression. This protocol describes the design and preparation of an NP platform for mitochondria-specific gene delivery (mito-NP). We use mito-NP in primary liver fibroblasts that are transplanted into mice. Mito-NP can be used to deliver various nucleic acid therapeutics and to treat mitochondria-regulated diseases. For complete details on the use and execution of this protocol, please refer to Zhao et al. (2020).
format Online
Article
Text
id pubmed-7817499
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Elsevier
record_format MEDLINE/PubMed
spelling pubmed-78174992021-01-27 Mitochondria-specific delivery system for targeted regulation of mitochondrial gene expression Su, Shicheng Cao, Shuwen Liu, Jiayu Xu, Xiaoding STAR Protoc Protocol Targeted regulation of mitochondrial gene expression is challenging due to the lack of a mitochondria-specific delivery system. We have previously developed various stimuli-responsive nanoparticle (NP)-based delivery systems to transport nucleic acids for regulation of target gene expression. This protocol describes the design and preparation of an NP platform for mitochondria-specific gene delivery (mito-NP). We use mito-NP in primary liver fibroblasts that are transplanted into mice. Mito-NP can be used to deliver various nucleic acid therapeutics and to treat mitochondria-regulated diseases. For complete details on the use and execution of this protocol, please refer to Zhao et al. (2020). Elsevier 2021-01-15 /pmc/articles/PMC7817499/ /pubmed/33511358 http://dx.doi.org/10.1016/j.xpro.2020.100275 Text en © 2020 The Author(s) http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Su, Shicheng
Cao, Shuwen
Liu, Jiayu
Xu, Xiaoding
Mitochondria-specific delivery system for targeted regulation of mitochondrial gene expression
title Mitochondria-specific delivery system for targeted regulation of mitochondrial gene expression
title_full Mitochondria-specific delivery system for targeted regulation of mitochondrial gene expression
title_fullStr Mitochondria-specific delivery system for targeted regulation of mitochondrial gene expression
title_full_unstemmed Mitochondria-specific delivery system for targeted regulation of mitochondrial gene expression
title_short Mitochondria-specific delivery system for targeted regulation of mitochondrial gene expression
title_sort mitochondria-specific delivery system for targeted regulation of mitochondrial gene expression
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7817499/
https://www.ncbi.nlm.nih.gov/pubmed/33511358
http://dx.doi.org/10.1016/j.xpro.2020.100275
work_keys_str_mv AT sushicheng mitochondriaspecificdeliverysystemfortargetedregulationofmitochondrialgeneexpression
AT caoshuwen mitochondriaspecificdeliverysystemfortargetedregulationofmitochondrialgeneexpression
AT liujiayu mitochondriaspecificdeliverysystemfortargetedregulationofmitochondrialgeneexpression
AT xuxiaoding mitochondriaspecificdeliverysystemfortargetedregulationofmitochondrialgeneexpression

Ejemplares similares