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Anti-Influenza virus effects of Enterococcus faecalis KH2 and Lactobacillus plantarum SNK12 RNA

Bacterial RNA has recently emerged as an immune-stimulating factor during viral infection. The immune response in an organism is directly related to the progression of virus infections. Lactic acid bacteria in particular have anticancer, bioprotective, and antiallergic effects by modulating immunity...

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Autores principales: WATANABE, Takumi, HAYASHI, Kyoko, KAN, Tatsuhiko, OHWAKI, Makoto, KAWAHARA, Toshio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BMFH Press 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7817512/
https://www.ncbi.nlm.nih.gov/pubmed/33520568
http://dx.doi.org/10.12938/bmfh.2020-019
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author WATANABE, Takumi
HAYASHI, Kyoko
KAN, Tatsuhiko
OHWAKI, Makoto
KAWAHARA, Toshio
author_facet WATANABE, Takumi
HAYASHI, Kyoko
KAN, Tatsuhiko
OHWAKI, Makoto
KAWAHARA, Toshio
author_sort WATANABE, Takumi
collection PubMed
description Bacterial RNA has recently emerged as an immune-stimulating factor during viral infection. The immune response in an organism is directly related to the progression of virus infections. Lactic acid bacteria in particular have anticancer, bioprotective, and antiallergic effects by modulating immunity. Here, we aimed to demonstrate the effect of bacterial RNA on in vitro production of IL-12, a proinflammatory cytokine, and on in vivo activity against influenza A virus (IFV) infection. Oral administration of heat-killed Enterococcus faecalis KH2 (KH2) or Lactobacillus plantarum SNK12 (SNK) in IFV-infected mice suppressed viral replication and stimulated production of virus-specific antibodies. However, ribonuclease-treated KH2 or SNK abrogated the effect, reducing IL-12 production in vitro and anti-IFV effects in vivo. Taken together, KH2 or SNK showed antiviral effects in vivo when administered orally, and the RNAs of KH2 and SNK play a part in these effects, despite the phylogenetic differences between the bacteria.
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spelling pubmed-78175122021-01-28 Anti-Influenza virus effects of Enterococcus faecalis KH2 and Lactobacillus plantarum SNK12 RNA WATANABE, Takumi HAYASHI, Kyoko KAN, Tatsuhiko OHWAKI, Makoto KAWAHARA, Toshio Biosci Microbiota Food Health Full Paper Bacterial RNA has recently emerged as an immune-stimulating factor during viral infection. The immune response in an organism is directly related to the progression of virus infections. Lactic acid bacteria in particular have anticancer, bioprotective, and antiallergic effects by modulating immunity. Here, we aimed to demonstrate the effect of bacterial RNA on in vitro production of IL-12, a proinflammatory cytokine, and on in vivo activity against influenza A virus (IFV) infection. Oral administration of heat-killed Enterococcus faecalis KH2 (KH2) or Lactobacillus plantarum SNK12 (SNK) in IFV-infected mice suppressed viral replication and stimulated production of virus-specific antibodies. However, ribonuclease-treated KH2 or SNK abrogated the effect, reducing IL-12 production in vitro and anti-IFV effects in vivo. Taken together, KH2 or SNK showed antiviral effects in vivo when administered orally, and the RNAs of KH2 and SNK play a part in these effects, despite the phylogenetic differences between the bacteria. BMFH Press 2020-09-12 2021 /pmc/articles/PMC7817512/ /pubmed/33520568 http://dx.doi.org/10.12938/bmfh.2020-019 Text en ©2021 BMFH Press This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. (CC-BY-NC-ND 4.0: https://creativecommons.org/licenses/by-nc-nd/4.0/)
spellingShingle Full Paper
WATANABE, Takumi
HAYASHI, Kyoko
KAN, Tatsuhiko
OHWAKI, Makoto
KAWAHARA, Toshio
Anti-Influenza virus effects of Enterococcus faecalis KH2 and Lactobacillus plantarum SNK12 RNA
title Anti-Influenza virus effects of Enterococcus faecalis KH2 and Lactobacillus plantarum SNK12 RNA
title_full Anti-Influenza virus effects of Enterococcus faecalis KH2 and Lactobacillus plantarum SNK12 RNA
title_fullStr Anti-Influenza virus effects of Enterococcus faecalis KH2 and Lactobacillus plantarum SNK12 RNA
title_full_unstemmed Anti-Influenza virus effects of Enterococcus faecalis KH2 and Lactobacillus plantarum SNK12 RNA
title_short Anti-Influenza virus effects of Enterococcus faecalis KH2 and Lactobacillus plantarum SNK12 RNA
title_sort anti-influenza virus effects of enterococcus faecalis kh2 and lactobacillus plantarum snk12 rna
topic Full Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7817512/
https://www.ncbi.nlm.nih.gov/pubmed/33520568
http://dx.doi.org/10.12938/bmfh.2020-019
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