STIM-Orai1 signaling regulates fluidity of cytoplasm during membrane blebbing

The cytoplasm in mammalian cells is considered homogeneous. In this study, we report that the cytoplasmic fluidity is regulated in the blebbing cells; the cytoplasm of rapidly expanding membrane blebs is more disordered than the cytoplasm of retracting blebs. The increase of cytoplasmic fluidity in...

Descripción completa

Detalles Bibliográficos
Autores principales: Aoki, Kana, Harada, Shota, Kawaji, Keita, Matsuzawa, Kenji, Uchida, Seiichi, Ikenouchi, Junichi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7817837/
https://www.ncbi.nlm.nih.gov/pubmed/33473127
http://dx.doi.org/10.1038/s41467-020-20826-5
_version_ 1783638717492101120
author Aoki, Kana
Harada, Shota
Kawaji, Keita
Matsuzawa, Kenji
Uchida, Seiichi
Ikenouchi, Junichi
author_facet Aoki, Kana
Harada, Shota
Kawaji, Keita
Matsuzawa, Kenji
Uchida, Seiichi
Ikenouchi, Junichi
author_sort Aoki, Kana
collection PubMed
description The cytoplasm in mammalian cells is considered homogeneous. In this study, we report that the cytoplasmic fluidity is regulated in the blebbing cells; the cytoplasm of rapidly expanding membrane blebs is more disordered than the cytoplasm of retracting blebs. The increase of cytoplasmic fluidity in the expanding bleb is caused by a sharp rise in the calcium concentration. The STIM-Orai1 pathway regulates this rapid and restricted increase of calcium in the expanding blebs. Conversely, activated ERM protein binds to Orai1 to inhibit the store-operated calcium entry in retracting blebs, which results in decreased in cytoplasmic calcium, rapid reassembly of the actin cortex.
format Online
Article
Text
id pubmed-7817837
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Nature Publishing Group UK
record_format MEDLINE/PubMed
spelling pubmed-78178372021-01-28 STIM-Orai1 signaling regulates fluidity of cytoplasm during membrane blebbing Aoki, Kana Harada, Shota Kawaji, Keita Matsuzawa, Kenji Uchida, Seiichi Ikenouchi, Junichi Nat Commun Article The cytoplasm in mammalian cells is considered homogeneous. In this study, we report that the cytoplasmic fluidity is regulated in the blebbing cells; the cytoplasm of rapidly expanding membrane blebs is more disordered than the cytoplasm of retracting blebs. The increase of cytoplasmic fluidity in the expanding bleb is caused by a sharp rise in the calcium concentration. The STIM-Orai1 pathway regulates this rapid and restricted increase of calcium in the expanding blebs. Conversely, activated ERM protein binds to Orai1 to inhibit the store-operated calcium entry in retracting blebs, which results in decreased in cytoplasmic calcium, rapid reassembly of the actin cortex. Nature Publishing Group UK 2021-01-20 /pmc/articles/PMC7817837/ /pubmed/33473127 http://dx.doi.org/10.1038/s41467-020-20826-5 Text en © The Author(s) 2021 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Aoki, Kana
Harada, Shota
Kawaji, Keita
Matsuzawa, Kenji
Uchida, Seiichi
Ikenouchi, Junichi
STIM-Orai1 signaling regulates fluidity of cytoplasm during membrane blebbing
title STIM-Orai1 signaling regulates fluidity of cytoplasm during membrane blebbing
title_full STIM-Orai1 signaling regulates fluidity of cytoplasm during membrane blebbing
title_fullStr STIM-Orai1 signaling regulates fluidity of cytoplasm during membrane blebbing
title_full_unstemmed STIM-Orai1 signaling regulates fluidity of cytoplasm during membrane blebbing
title_short STIM-Orai1 signaling regulates fluidity of cytoplasm during membrane blebbing
title_sort stim-orai1 signaling regulates fluidity of cytoplasm during membrane blebbing
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7817837/
https://www.ncbi.nlm.nih.gov/pubmed/33473127
http://dx.doi.org/10.1038/s41467-020-20826-5
work_keys_str_mv AT aokikana stimorai1signalingregulatesfluidityofcytoplasmduringmembraneblebbing
AT haradashota stimorai1signalingregulatesfluidityofcytoplasmduringmembraneblebbing
AT kawajikeita stimorai1signalingregulatesfluidityofcytoplasmduringmembraneblebbing
AT matsuzawakenji stimorai1signalingregulatesfluidityofcytoplasmduringmembraneblebbing
AT uchidaseiichi stimorai1signalingregulatesfluidityofcytoplasmduringmembraneblebbing
AT ikenouchijunichi stimorai1signalingregulatesfluidityofcytoplasmduringmembraneblebbing

Ejemplares similares