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Use of the Retinal Vascular Histology to Validate an Optical Coherence Tomography Angiography Technique

PURPOSE: To determine the fidelity of optical coherence tomography angiography (OCTA) techniques by direct comparison of the retinal capillary network images obtained from the same region as imaged by OCTA and high-resolution confocal microscope. METHOD: Ten porcine eyes were perfused with red blood...

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Autores principales: Yu, Paula K., Mehnert, Andrew, Athwal, Arman, Sarunic, Marinko V., Yu, Dao-Yi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Association for Research in Vision and Ophthalmology 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7817878/
https://www.ncbi.nlm.nih.gov/pubmed/33520424
http://dx.doi.org/10.1167/tvst.10.1.29
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author Yu, Paula K.
Mehnert, Andrew
Athwal, Arman
Sarunic, Marinko V.
Yu, Dao-Yi
author_facet Yu, Paula K.
Mehnert, Andrew
Athwal, Arman
Sarunic, Marinko V.
Yu, Dao-Yi
author_sort Yu, Paula K.
collection PubMed
description PURPOSE: To determine the fidelity of optical coherence tomography angiography (OCTA) techniques by direct comparison of the retinal capillary network images obtained from the same region as imaged by OCTA and high-resolution confocal microscope. METHOD: Ten porcine eyes were perfused with red blood cells for OCTA image acquisition from the area centralis and then perfusion-fixed, and the vessels were labeled for confocal imaging. Two approaches involving post-processing of two-dimensional projection images and vessel tracking on three dimensional image stacks were used to obtain quantitative measurements. Data collected include vessel density, length of visible vessel track, count of visible branch points, vessel track depth, vessel diameter, angle of vessel descent, and angle of dive for comparison and analysis. RESULTS: Comparing vascular images acquired from OCTA and confocal microscopy, we found (1) a good representation of the larger caliber retinal vessels, (2) an underrepresentation of retinal microvessels smaller than 10 µm and branch points in all four retinal vascular plexuses, particularly the intermediate capillary plexus, (3) reduced visibility associated with an increase in the angle of descent, (4) a tendency to loss visibility of vessel track at a branch point or during a sharp dive, and (5) a reduction in visibility with increase in retinal depth on OCTA images. CONCLUSIONS: Current OCTA techniques can visualize the retinal capillary network, but some types of capillaries cannot be detected by OCTA, particularly in the middle to deeper layers. TRANSLATIONAL RELEVANCE: The information indicates the limitation in clinical use and scopes for improvement in the current OCTA technologies.
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spelling pubmed-78178782021-01-29 Use of the Retinal Vascular Histology to Validate an Optical Coherence Tomography Angiography Technique Yu, Paula K. Mehnert, Andrew Athwal, Arman Sarunic, Marinko V. Yu, Dao-Yi Transl Vis Sci Technol Article PURPOSE: To determine the fidelity of optical coherence tomography angiography (OCTA) techniques by direct comparison of the retinal capillary network images obtained from the same region as imaged by OCTA and high-resolution confocal microscope. METHOD: Ten porcine eyes were perfused with red blood cells for OCTA image acquisition from the area centralis and then perfusion-fixed, and the vessels were labeled for confocal imaging. Two approaches involving post-processing of two-dimensional projection images and vessel tracking on three dimensional image stacks were used to obtain quantitative measurements. Data collected include vessel density, length of visible vessel track, count of visible branch points, vessel track depth, vessel diameter, angle of vessel descent, and angle of dive for comparison and analysis. RESULTS: Comparing vascular images acquired from OCTA and confocal microscopy, we found (1) a good representation of the larger caliber retinal vessels, (2) an underrepresentation of retinal microvessels smaller than 10 µm and branch points in all four retinal vascular plexuses, particularly the intermediate capillary plexus, (3) reduced visibility associated with an increase in the angle of descent, (4) a tendency to loss visibility of vessel track at a branch point or during a sharp dive, and (5) a reduction in visibility with increase in retinal depth on OCTA images. CONCLUSIONS: Current OCTA techniques can visualize the retinal capillary network, but some types of capillaries cannot be detected by OCTA, particularly in the middle to deeper layers. TRANSLATIONAL RELEVANCE: The information indicates the limitation in clinical use and scopes for improvement in the current OCTA technologies. The Association for Research in Vision and Ophthalmology 2021-01-19 /pmc/articles/PMC7817878/ /pubmed/33520424 http://dx.doi.org/10.1167/tvst.10.1.29 Text en Copyright 2021 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
spellingShingle Article
Yu, Paula K.
Mehnert, Andrew
Athwal, Arman
Sarunic, Marinko V.
Yu, Dao-Yi
Use of the Retinal Vascular Histology to Validate an Optical Coherence Tomography Angiography Technique
title Use of the Retinal Vascular Histology to Validate an Optical Coherence Tomography Angiography Technique
title_full Use of the Retinal Vascular Histology to Validate an Optical Coherence Tomography Angiography Technique
title_fullStr Use of the Retinal Vascular Histology to Validate an Optical Coherence Tomography Angiography Technique
title_full_unstemmed Use of the Retinal Vascular Histology to Validate an Optical Coherence Tomography Angiography Technique
title_short Use of the Retinal Vascular Histology to Validate an Optical Coherence Tomography Angiography Technique
title_sort use of the retinal vascular histology to validate an optical coherence tomography angiography technique
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7817878/
https://www.ncbi.nlm.nih.gov/pubmed/33520424
http://dx.doi.org/10.1167/tvst.10.1.29
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