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Isobaric Labeling Proteomics Allows a High-Throughput Investigation of Protein Corona Orientation

[Image: see text] The formation of the biomolecular corona represents a crucial factor in controlling the biological interactions and trafficking of nanomaterials. In this context, the availability of key epitopes exposed on the surface of the corona, and able to engage the biological machinery, is...

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Autores principales: Liessi, Nara, Maragliano, Luca, Castagnola, Valentina, Bramini, Mattia, Benfenati, Fabio, Armirotti, Andrea
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2020
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7818227/
https://www.ncbi.nlm.nih.gov/pubmed/33285070
http://dx.doi.org/10.1021/acs.analchem.0c03134
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author Liessi, Nara
Maragliano, Luca
Castagnola, Valentina
Bramini, Mattia
Benfenati, Fabio
Armirotti, Andrea
author_facet Liessi, Nara
Maragliano, Luca
Castagnola, Valentina
Bramini, Mattia
Benfenati, Fabio
Armirotti, Andrea
author_sort Liessi, Nara
collection PubMed
description [Image: see text] The formation of the biomolecular corona represents a crucial factor in controlling the biological interactions and trafficking of nanomaterials. In this context, the availability of key epitopes exposed on the surface of the corona, and able to engage the biological machinery, is important to define the biological fate of the material. While the full biomolecular corona composition can be investigated by conventional bottom-up proteomics, the assessment of the spatial orientation of proteins in the corona in a high-throughput fashion is still challenging. In this work, we show that labeling corona proteins with isobaric tags in their native conditions and analyzing the MS/MS spectra of tryptic peptides allow an easy and high-throughput assessment of the inner/outer orientation of the corresponding proteins in the original corona. We put our results in the context of what is currently known of the protein corona of graphene-based nanomaterials. Our conclusions are in line with previous data and were confirmed by in silico calculations.
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spelling pubmed-78182272021-01-22 Isobaric Labeling Proteomics Allows a High-Throughput Investigation of Protein Corona Orientation Liessi, Nara Maragliano, Luca Castagnola, Valentina Bramini, Mattia Benfenati, Fabio Armirotti, Andrea Anal Chem [Image: see text] The formation of the biomolecular corona represents a crucial factor in controlling the biological interactions and trafficking of nanomaterials. In this context, the availability of key epitopes exposed on the surface of the corona, and able to engage the biological machinery, is important to define the biological fate of the material. While the full biomolecular corona composition can be investigated by conventional bottom-up proteomics, the assessment of the spatial orientation of proteins in the corona in a high-throughput fashion is still challenging. In this work, we show that labeling corona proteins with isobaric tags in their native conditions and analyzing the MS/MS spectra of tryptic peptides allow an easy and high-throughput assessment of the inner/outer orientation of the corresponding proteins in the original corona. We put our results in the context of what is currently known of the protein corona of graphene-based nanomaterials. Our conclusions are in line with previous data and were confirmed by in silico calculations. American Chemical Society 2020-12-07 2021-01-19 /pmc/articles/PMC7818227/ /pubmed/33285070 http://dx.doi.org/10.1021/acs.analchem.0c03134 Text en © 2020 American Chemical Society This is an open access article published under a Creative Commons Attribution (CC-BY) License (http://pubs.acs.org/page/policy/authorchoice_ccby_termsofuse.html) , which permits unrestricted use, distribution and reproduction in any medium, provided the author and source are cited.
spellingShingle Liessi, Nara
Maragliano, Luca
Castagnola, Valentina
Bramini, Mattia
Benfenati, Fabio
Armirotti, Andrea
Isobaric Labeling Proteomics Allows a High-Throughput Investigation of Protein Corona Orientation
title Isobaric Labeling Proteomics Allows a High-Throughput Investigation of Protein Corona Orientation
title_full Isobaric Labeling Proteomics Allows a High-Throughput Investigation of Protein Corona Orientation
title_fullStr Isobaric Labeling Proteomics Allows a High-Throughput Investigation of Protein Corona Orientation
title_full_unstemmed Isobaric Labeling Proteomics Allows a High-Throughput Investigation of Protein Corona Orientation
title_short Isobaric Labeling Proteomics Allows a High-Throughput Investigation of Protein Corona Orientation
title_sort isobaric labeling proteomics allows a high-throughput investigation of protein corona orientation
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7818227/
https://www.ncbi.nlm.nih.gov/pubmed/33285070
http://dx.doi.org/10.1021/acs.analchem.0c03134
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