Development of a protocol for detection of SARS-CoV-2 in sputum and endotracheal aspirates using Cepheid Xpert Xpress SARS-CoV-2

Sputum and endotracheal aspirates (ETs) are not among the vendor-approved specimens for the Cepheid Xpert SARS-CoV-2 assay. However, they are the common lower respiratory tract specimens submitted for laboratory diagnosis. Testing for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in l...

Descripción completa

Detalles Bibliográficos
Autores principales: Malczynski, Michael, Rezaeian, Saba, Rios, Javier, Dirnberger, Laura, Polanco, Wanda, Zembower, Teresa, Qi, Chao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Microbiology Society 2020
Materias:
Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7818244/
https://www.ncbi.nlm.nih.gov/pubmed/33490871
http://dx.doi.org/10.1099/acmi.0.000176
_version_ 1783638794641080320
author Malczynski, Michael
Rezaeian, Saba
Rios, Javier
Dirnberger, Laura
Polanco, Wanda
Zembower, Teresa
Qi, Chao
author_facet Malczynski, Michael
Rezaeian, Saba
Rios, Javier
Dirnberger, Laura
Polanco, Wanda
Zembower, Teresa
Qi, Chao
author_sort Malczynski, Michael
collection PubMed
description Sputum and endotracheal aspirates (ETs) are not among the vendor-approved specimens for the Cepheid Xpert SARS-CoV-2 assay. However, they are the common lower respiratory tract specimens submitted for laboratory diagnosis. Testing for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in lower respiratory tract samples is required for the discharge of patients from coronavirus disease (COVID) units at some institutions. We developed a protocol used for testing unliquified viscous sputum or tracheal aspirate with the Cepheid Xpert SARS-CoV-2 assay.
format Online
Article
Text
id pubmed-7818244
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher Microbiology Society
record_format MEDLINE/PubMed
spelling pubmed-78182442021-01-21 Development of a protocol for detection of SARS-CoV-2 in sputum and endotracheal aspirates using Cepheid Xpert Xpress SARS-CoV-2 Malczynski, Michael Rezaeian, Saba Rios, Javier Dirnberger, Laura Polanco, Wanda Zembower, Teresa Qi, Chao Access Microbiol Short Communication Sputum and endotracheal aspirates (ETs) are not among the vendor-approved specimens for the Cepheid Xpert SARS-CoV-2 assay. However, they are the common lower respiratory tract specimens submitted for laboratory diagnosis. Testing for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in lower respiratory tract samples is required for the discharge of patients from coronavirus disease (COVID) units at some institutions. We developed a protocol used for testing unliquified viscous sputum or tracheal aspirate with the Cepheid Xpert SARS-CoV-2 assay. Microbiology Society 2020-11-12 /pmc/articles/PMC7818244/ /pubmed/33490871 http://dx.doi.org/10.1099/acmi.0.000176 Text en © 2020 The Authors https://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution NonCommercial License. The Microbiology Society waived the open access fees for this article.
spellingShingle Short Communication
Malczynski, Michael
Rezaeian, Saba
Rios, Javier
Dirnberger, Laura
Polanco, Wanda
Zembower, Teresa
Qi, Chao
Development of a protocol for detection of SARS-CoV-2 in sputum and endotracheal aspirates using Cepheid Xpert Xpress SARS-CoV-2
title Development of a protocol for detection of SARS-CoV-2 in sputum and endotracheal aspirates using Cepheid Xpert Xpress SARS-CoV-2
title_full Development of a protocol for detection of SARS-CoV-2 in sputum and endotracheal aspirates using Cepheid Xpert Xpress SARS-CoV-2
title_fullStr Development of a protocol for detection of SARS-CoV-2 in sputum and endotracheal aspirates using Cepheid Xpert Xpress SARS-CoV-2
title_full_unstemmed Development of a protocol for detection of SARS-CoV-2 in sputum and endotracheal aspirates using Cepheid Xpert Xpress SARS-CoV-2
title_short Development of a protocol for detection of SARS-CoV-2 in sputum and endotracheal aspirates using Cepheid Xpert Xpress SARS-CoV-2
title_sort development of a protocol for detection of sars-cov-2 in sputum and endotracheal aspirates using cepheid xpert xpress sars-cov-2
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7818244/
https://www.ncbi.nlm.nih.gov/pubmed/33490871
http://dx.doi.org/10.1099/acmi.0.000176
work_keys_str_mv AT malczynskimichael developmentofaprotocolfordetectionofsarscov2insputumandendotrachealaspiratesusingcepheidxpertxpresssarscov2
AT rezaeiansaba developmentofaprotocolfordetectionofsarscov2insputumandendotrachealaspiratesusingcepheidxpertxpresssarscov2
AT riosjavier developmentofaprotocolfordetectionofsarscov2insputumandendotrachealaspiratesusingcepheidxpertxpresssarscov2
AT dirnbergerlaura developmentofaprotocolfordetectionofsarscov2insputumandendotrachealaspiratesusingcepheidxpertxpresssarscov2
AT polancowanda developmentofaprotocolfordetectionofsarscov2insputumandendotrachealaspiratesusingcepheidxpertxpresssarscov2
AT zembowerteresa developmentofaprotocolfordetectionofsarscov2insputumandendotrachealaspiratesusingcepheidxpertxpresssarscov2
AT qichao developmentofaprotocolfordetectionofsarscov2insputumandendotrachealaspiratesusingcepheidxpertxpresssarscov2

Ejemplares similares