Stem cell behaviors on periodic arrays of nanopillars analyzed by high-resolution scanning electron microscope images

The biocompatible polyurethane acrylate (PUA) nanopillars were fabricated by soft lithography using three different sizes of nanobeads (350, 500, and 1000 nm), and the human adipose-derived stem cells (hASCs) were cultured on the nanopillars. The hASCs and their various behaviors, such as cytoplasmi...

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Autores principales: Kang, Jihun, Kang, Eun-Hye, Yun, Young-Shik, Ji, Seungmuk, Yun, In-Sik, Yeo, Jong-Souk
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Singapore 2020
Materias:
Novus Imago
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7818290/
https://www.ncbi.nlm.nih.gov/pubmed/33580331
http://dx.doi.org/10.1186/s42649-020-00046-3
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author Kang, Jihun
Kang, Eun-Hye
Yun, Young-Shik
Ji, Seungmuk
Yun, In-Sik
Yeo, Jong-Souk
author_facet Kang, Jihun
Kang, Eun-Hye
Yun, Young-Shik
Ji, Seungmuk
Yun, In-Sik
Yeo, Jong-Souk
author_sort Kang, Jihun
collection PubMed
description The biocompatible polyurethane acrylate (PUA) nanopillars were fabricated by soft lithography using three different sizes of nanobeads (350, 500, and 1000 nm), and the human adipose-derived stem cells (hASCs) were cultured on the nanopillars. The hASCs and their various behaviors, such as cytoplasmic projections, migration, and morphology, were observed by high resolution images using a scanning electron microscope (SEM). With the accurate analysis by SEM for the controlled sizes of nanopillars, the deflections are observed at pillars fabricated with 350- and 500-nm nanobeads. These high-resolution images could offer crucial information to elucidate the complicated correlations between nanopillars and the cells, such as morphology and cytoplasmic projections.
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spelling pubmed-78182902021-02-10 Stem cell behaviors on periodic arrays of nanopillars analyzed by high-resolution scanning electron microscope images Kang, Jihun Kang, Eun-Hye Yun, Young-Shik Ji, Seungmuk Yun, In-Sik Yeo, Jong-Souk Appl Microsc Novus Imago The biocompatible polyurethane acrylate (PUA) nanopillars were fabricated by soft lithography using three different sizes of nanobeads (350, 500, and 1000 nm), and the human adipose-derived stem cells (hASCs) were cultured on the nanopillars. The hASCs and their various behaviors, such as cytoplasmic projections, migration, and morphology, were observed by high resolution images using a scanning electron microscope (SEM). With the accurate analysis by SEM for the controlled sizes of nanopillars, the deflections are observed at pillars fabricated with 350- and 500-nm nanobeads. These high-resolution images could offer crucial information to elucidate the complicated correlations between nanopillars and the cells, such as morphology and cytoplasmic projections. Springer Singapore 2020-11-17 /pmc/articles/PMC7818290/ /pubmed/33580331 http://dx.doi.org/10.1186/s42649-020-00046-3 Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Novus Imago
Kang, Jihun
Kang, Eun-Hye
Yun, Young-Shik
Ji, Seungmuk
Yun, In-Sik
Yeo, Jong-Souk
Stem cell behaviors on periodic arrays of nanopillars analyzed by high-resolution scanning electron microscope images
title Stem cell behaviors on periodic arrays of nanopillars analyzed by high-resolution scanning electron microscope images
title_full Stem cell behaviors on periodic arrays of nanopillars analyzed by high-resolution scanning electron microscope images
title_fullStr Stem cell behaviors on periodic arrays of nanopillars analyzed by high-resolution scanning electron microscope images
title_full_unstemmed Stem cell behaviors on periodic arrays of nanopillars analyzed by high-resolution scanning electron microscope images
title_short Stem cell behaviors on periodic arrays of nanopillars analyzed by high-resolution scanning electron microscope images
title_sort stem cell behaviors on periodic arrays of nanopillars analyzed by high-resolution scanning electron microscope images
topic Novus Imago
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7818290/
https://www.ncbi.nlm.nih.gov/pubmed/33580331
http://dx.doi.org/10.1186/s42649-020-00046-3
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