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Methanol fixation for scanning electron microscopy of plants
Plant specimens for scanning electron microscopy (SEM) are commonly treated using standard protocols. Conventional fixatives consist of toxic chemicals such as glutaraldehyde, paraformaldehyde, and osmium tetroxide. In 1996, methanol fixation was reported as a rapid alternative to the standard proto...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Springer Singapore
2020
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7818355/ https://www.ncbi.nlm.nih.gov/pubmed/33580311 http://dx.doi.org/10.1186/s42649-020-00028-5 |
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author | Kim, Ki Woo |
author_facet | Kim, Ki Woo |
author_sort | Kim, Ki Woo |
collection | PubMed |
description | Plant specimens for scanning electron microscopy (SEM) are commonly treated using standard protocols. Conventional fixatives consist of toxic chemicals such as glutaraldehyde, paraformaldehyde, and osmium tetroxide. In 1996, methanol fixation was reported as a rapid alternative to the standard protocols. If specimens are immersed in methanol for 30 s or longer and critical-point dried, they appear to be comparable in preservation quality to those treated with the chemical fixatives. A modified version that consists of methanol fixation and ethanol dehydration was effective at preserving the tissue morphology and dimensions. These solvent-based fixation and dehydration protocols are regarded as rapid and simple alternatives to standard protocols for SEM of plants. |
format | Online Article Text |
id | pubmed-7818355 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Springer Singapore |
record_format | MEDLINE/PubMed |
spelling | pubmed-78183552021-02-10 Methanol fixation for scanning electron microscopy of plants Kim, Ki Woo Appl Microsc Review Plant specimens for scanning electron microscopy (SEM) are commonly treated using standard protocols. Conventional fixatives consist of toxic chemicals such as glutaraldehyde, paraformaldehyde, and osmium tetroxide. In 1996, methanol fixation was reported as a rapid alternative to the standard protocols. If specimens are immersed in methanol for 30 s or longer and critical-point dried, they appear to be comparable in preservation quality to those treated with the chemical fixatives. A modified version that consists of methanol fixation and ethanol dehydration was effective at preserving the tissue morphology and dimensions. These solvent-based fixation and dehydration protocols are regarded as rapid and simple alternatives to standard protocols for SEM of plants. Springer Singapore 2020-05-25 /pmc/articles/PMC7818355/ /pubmed/33580311 http://dx.doi.org/10.1186/s42649-020-00028-5 Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Review Kim, Ki Woo Methanol fixation for scanning electron microscopy of plants |
title | Methanol fixation for scanning electron microscopy of plants |
title_full | Methanol fixation for scanning electron microscopy of plants |
title_fullStr | Methanol fixation for scanning electron microscopy of plants |
title_full_unstemmed | Methanol fixation for scanning electron microscopy of plants |
title_short | Methanol fixation for scanning electron microscopy of plants |
title_sort | methanol fixation for scanning electron microscopy of plants |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7818355/ https://www.ncbi.nlm.nih.gov/pubmed/33580311 http://dx.doi.org/10.1186/s42649-020-00028-5 |
work_keys_str_mv | AT kimkiwoo methanolfixationforscanningelectronmicroscopyofplants |