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Mouse parotid salivary gland organoids for the in vitro study of stem cell radiation response
OBJECTIVE: Hyposalivation‐related xerostomia is an irreversible, untreatable, and frequent condition after radiotherapy for head and neck cancer. Stem cell therapy is an attractive option of treatment, but demands knowledge of stem cell functioning. Therefore, we aimed to develop a murine parotid gl...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7818507/ https://www.ncbi.nlm.nih.gov/pubmed/32531849 http://dx.doi.org/10.1111/odi.13475 |
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author | Serrano Martinez, Paola Cinat, Davide van Luijk, Peter Baanstra, Mirjam de Haan, Gerald Pringle, Sarah Coppes, Robert P. |
author_facet | Serrano Martinez, Paola Cinat, Davide van Luijk, Peter Baanstra, Mirjam de Haan, Gerald Pringle, Sarah Coppes, Robert P. |
author_sort | Serrano Martinez, Paola |
collection | PubMed |
description | OBJECTIVE: Hyposalivation‐related xerostomia is an irreversible, untreatable, and frequent condition after radiotherapy for head and neck cancer. Stem cell therapy is an attractive option of treatment, but demands knowledge of stem cell functioning. Therefore, we aimed to develop a murine parotid gland organoid model to explore radiation response of stem cells in vitro. MATERIALS AND METHODS: Single cells derived from murine parotid gland organoids were passaged in Matrigel with defined medium to assess self‐renewal and differentiation potential. Single cells were irradiated and plated in a 3D clonogenic stem cell survival assay to assess submandibular and parotid gland radiation response. RESULTS: Single cells derived from parotid gland organoids were able to extensively self‐renew and differentiate into all major tissue cell types, indicating the presence of potential stem cells. FACS selection for known salivary gland stem cell markers CD24/CD29 did not further enrich for stem cells. The parotid gland organoid‐derived stem cells displayed radiation dose–response curves similar to the submandibular gland. CONCLUSIONS: Murine parotid gland organoids harbor stem cells with long‐term expansion and differentiation potential. This model is useful for mechanistic studies of stem cell radiation response and suggests similar radiosensitivity for the parotid and submandibular gland organoids. |
format | Online Article Text |
id | pubmed-7818507 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-78185072021-01-26 Mouse parotid salivary gland organoids for the in vitro study of stem cell radiation response Serrano Martinez, Paola Cinat, Davide van Luijk, Peter Baanstra, Mirjam de Haan, Gerald Pringle, Sarah Coppes, Robert P. Oral Dis Saliva and Salivary Glands OBJECTIVE: Hyposalivation‐related xerostomia is an irreversible, untreatable, and frequent condition after radiotherapy for head and neck cancer. Stem cell therapy is an attractive option of treatment, but demands knowledge of stem cell functioning. Therefore, we aimed to develop a murine parotid gland organoid model to explore radiation response of stem cells in vitro. MATERIALS AND METHODS: Single cells derived from murine parotid gland organoids were passaged in Matrigel with defined medium to assess self‐renewal and differentiation potential. Single cells were irradiated and plated in a 3D clonogenic stem cell survival assay to assess submandibular and parotid gland radiation response. RESULTS: Single cells derived from parotid gland organoids were able to extensively self‐renew and differentiate into all major tissue cell types, indicating the presence of potential stem cells. FACS selection for known salivary gland stem cell markers CD24/CD29 did not further enrich for stem cells. The parotid gland organoid‐derived stem cells displayed radiation dose–response curves similar to the submandibular gland. CONCLUSIONS: Murine parotid gland organoids harbor stem cells with long‐term expansion and differentiation potential. This model is useful for mechanistic studies of stem cell radiation response and suggests similar radiosensitivity for the parotid and submandibular gland organoids. John Wiley and Sons Inc. 2020-06-29 2021-01 /pmc/articles/PMC7818507/ /pubmed/32531849 http://dx.doi.org/10.1111/odi.13475 Text en © 2020 The Authors. Oral Diseases published by John Wiley & Sons Ltd This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Saliva and Salivary Glands Serrano Martinez, Paola Cinat, Davide van Luijk, Peter Baanstra, Mirjam de Haan, Gerald Pringle, Sarah Coppes, Robert P. Mouse parotid salivary gland organoids for the in vitro study of stem cell radiation response |
title | Mouse parotid salivary gland organoids for the in vitro study of stem cell radiation response |
title_full | Mouse parotid salivary gland organoids for the in vitro study of stem cell radiation response |
title_fullStr | Mouse parotid salivary gland organoids for the in vitro study of stem cell radiation response |
title_full_unstemmed | Mouse parotid salivary gland organoids for the in vitro study of stem cell radiation response |
title_short | Mouse parotid salivary gland organoids for the in vitro study of stem cell radiation response |
title_sort | mouse parotid salivary gland organoids for the in vitro study of stem cell radiation response |
topic | Saliva and Salivary Glands |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7818507/ https://www.ncbi.nlm.nih.gov/pubmed/32531849 http://dx.doi.org/10.1111/odi.13475 |
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