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Pathogenicity of novel goose-origin astrovirus causing gout in goslings
BACKGROUND: A novel goose-origin astrovirus (GoAstV) has broken out across China in recent years, causing gout in goslings with a mortality rate of around 50%. However, our understanding of the dynamic distribution, tissue tropism and pathogenesis of GoAstV is incomplete. In order to assess its path...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7818743/ https://www.ncbi.nlm.nih.gov/pubmed/33472629 http://dx.doi.org/10.1186/s12917-020-02739-z |
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author | Yin, Dan Tian, Jiajun Yang, Jing Tang, Yi Diao, Youxiang |
author_facet | Yin, Dan Tian, Jiajun Yang, Jing Tang, Yi Diao, Youxiang |
author_sort | Yin, Dan |
collection | PubMed |
description | BACKGROUND: A novel goose-origin astrovirus (GoAstV) has broken out across China in recent years, causing gout in goslings with a mortality rate of around 50%. However, our understanding of the dynamic distribution, tissue tropism and pathogenesis of GoAstV is incomplete. In order to assess its pathogenicity, one-day-old goslings were inoculated separately with GoAstV via oral and subcutaneous injection routes. RESULTS: Clinical symptoms, gross and microscopic lesions, blood biochemical parameters and viral loads were detected and recorded for 20 days after infection. Typical gout was observed in experimental goslings. GoAstV can be replicated in tissues and cause pathological damage, especially in the kidney, liver, heart and spleen. Virus-specific genomic RNA was detected in blood, cloacal swabs and all representative tissues, and virus shedding was detected up to 20 days after inoculation, suggesting that GoAstV has a wide tissue tropism and spread systematically after inoculation. The viral copy numbers examined in kidney were the highest, followed by spleen and liver. CONCLUSION: This experiment determined the accurate value of viral loads and biochemical indicators of GoAstV-induced goslings. These findings increase our understanding of the pathogenicity of GoAstV in goslings and provide more reference for future research. |
format | Online Article Text |
id | pubmed-7818743 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-78187432021-01-22 Pathogenicity of novel goose-origin astrovirus causing gout in goslings Yin, Dan Tian, Jiajun Yang, Jing Tang, Yi Diao, Youxiang BMC Vet Res Research Article BACKGROUND: A novel goose-origin astrovirus (GoAstV) has broken out across China in recent years, causing gout in goslings with a mortality rate of around 50%. However, our understanding of the dynamic distribution, tissue tropism and pathogenesis of GoAstV is incomplete. In order to assess its pathogenicity, one-day-old goslings were inoculated separately with GoAstV via oral and subcutaneous injection routes. RESULTS: Clinical symptoms, gross and microscopic lesions, blood biochemical parameters and viral loads were detected and recorded for 20 days after infection. Typical gout was observed in experimental goslings. GoAstV can be replicated in tissues and cause pathological damage, especially in the kidney, liver, heart and spleen. Virus-specific genomic RNA was detected in blood, cloacal swabs and all representative tissues, and virus shedding was detected up to 20 days after inoculation, suggesting that GoAstV has a wide tissue tropism and spread systematically after inoculation. The viral copy numbers examined in kidney were the highest, followed by spleen and liver. CONCLUSION: This experiment determined the accurate value of viral loads and biochemical indicators of GoAstV-induced goslings. These findings increase our understanding of the pathogenicity of GoAstV in goslings and provide more reference for future research. BioMed Central 2021-01-20 /pmc/articles/PMC7818743/ /pubmed/33472629 http://dx.doi.org/10.1186/s12917-020-02739-z Text en © The Author(s) 2021 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Article Yin, Dan Tian, Jiajun Yang, Jing Tang, Yi Diao, Youxiang Pathogenicity of novel goose-origin astrovirus causing gout in goslings |
title | Pathogenicity of novel goose-origin astrovirus causing gout in goslings |
title_full | Pathogenicity of novel goose-origin astrovirus causing gout in goslings |
title_fullStr | Pathogenicity of novel goose-origin astrovirus causing gout in goslings |
title_full_unstemmed | Pathogenicity of novel goose-origin astrovirus causing gout in goslings |
title_short | Pathogenicity of novel goose-origin astrovirus causing gout in goslings |
title_sort | pathogenicity of novel goose-origin astrovirus causing gout in goslings |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7818743/ https://www.ncbi.nlm.nih.gov/pubmed/33472629 http://dx.doi.org/10.1186/s12917-020-02739-z |
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