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Transcriptomic analysis of s-methoprene resistance in the lesser grain borer, Rhyzopertha dominica, and evaluation of piperonyl butoxide as a resistance breaker

BACKGROUND: The lesser grain borer, Rhyzopertha dominica is a serious pest of stored grains. Fumigation and contact insecticides play a major role in managing this pest globally. While insects are developing genetic resistance to chemicals, hormonal analogues such as s-methoprene play a key role in...

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Autores principales: Sakka, Maria K., Riga, Maria, Ioannidis, Panagiotis, Baliota, Georgia V., Tselika, Martha, Jagadeesan, Rajeswaran, Nayak, Manoj K., Vontas, John, Athanassiou, Christos G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7819154/
https://www.ncbi.nlm.nih.gov/pubmed/33472593
http://dx.doi.org/10.1186/s12864-020-07354-8
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author Sakka, Maria K.
Riga, Maria
Ioannidis, Panagiotis
Baliota, Georgia V.
Tselika, Martha
Jagadeesan, Rajeswaran
Nayak, Manoj K.
Vontas, John
Athanassiou, Christos G.
author_facet Sakka, Maria K.
Riga, Maria
Ioannidis, Panagiotis
Baliota, Georgia V.
Tselika, Martha
Jagadeesan, Rajeswaran
Nayak, Manoj K.
Vontas, John
Athanassiou, Christos G.
author_sort Sakka, Maria K.
collection PubMed
description BACKGROUND: The lesser grain borer, Rhyzopertha dominica is a serious pest of stored grains. Fumigation and contact insecticides play a major role in managing this pest globally. While insects are developing genetic resistance to chemicals, hormonal analogues such as s-methoprene play a key role in reducing general pest pressure as well as managing pest populations that are resistant to fumigants and neurotoxic contact insecticides. However, resistance to s-methoprene has been reported in R. dominica with some reports showing a remarkable high resistance, questioning the use of this compound and other related analogues in grain protection. The current study attempts to identify possible molecular mechanisms that contribute in resistance to s-methoprene in R. dominica. RESULTS: Transcriptome analysis of resistant and susceptible strains of this pest species identified a set of differentially expressed genes related to cytochrome P450s, indicating their potential role in resistance to s-methoprene. Laboratory bioassays were performed with s-methoprene treated wheat grains in presence and absence of piperonyl butoxide (PBO), a cytochrome P450 inhibitor. The results indicate that PBO, when applied alone, at least at the concentration tested here, had no effect on R. dominica adult emergence, but has a clear synergistic effect to s-methoprene. The number of produced progeny decreased in presence of the inhibitor, especially in the resistant strain. In addition, we also identified CYP complement (CYPome) of R. dominica, annotated and analysed phylogenetically, to understand the evolutionary relationships with other species. CONCLUSIONS: The information generated in current study suggest that PBO can effectively be used to break resistance to s-methoprene in R. dominica. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12864-020-07354-8.
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spelling pubmed-78191542021-01-22 Transcriptomic analysis of s-methoprene resistance in the lesser grain borer, Rhyzopertha dominica, and evaluation of piperonyl butoxide as a resistance breaker Sakka, Maria K. Riga, Maria Ioannidis, Panagiotis Baliota, Georgia V. Tselika, Martha Jagadeesan, Rajeswaran Nayak, Manoj K. Vontas, John Athanassiou, Christos G. BMC Genomics Research Article BACKGROUND: The lesser grain borer, Rhyzopertha dominica is a serious pest of stored grains. Fumigation and contact insecticides play a major role in managing this pest globally. While insects are developing genetic resistance to chemicals, hormonal analogues such as s-methoprene play a key role in reducing general pest pressure as well as managing pest populations that are resistant to fumigants and neurotoxic contact insecticides. However, resistance to s-methoprene has been reported in R. dominica with some reports showing a remarkable high resistance, questioning the use of this compound and other related analogues in grain protection. The current study attempts to identify possible molecular mechanisms that contribute in resistance to s-methoprene in R. dominica. RESULTS: Transcriptome analysis of resistant and susceptible strains of this pest species identified a set of differentially expressed genes related to cytochrome P450s, indicating their potential role in resistance to s-methoprene. Laboratory bioassays were performed with s-methoprene treated wheat grains in presence and absence of piperonyl butoxide (PBO), a cytochrome P450 inhibitor. The results indicate that PBO, when applied alone, at least at the concentration tested here, had no effect on R. dominica adult emergence, but has a clear synergistic effect to s-methoprene. The number of produced progeny decreased in presence of the inhibitor, especially in the resistant strain. In addition, we also identified CYP complement (CYPome) of R. dominica, annotated and analysed phylogenetically, to understand the evolutionary relationships with other species. CONCLUSIONS: The information generated in current study suggest that PBO can effectively be used to break resistance to s-methoprene in R. dominica. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12864-020-07354-8. BioMed Central 2021-01-20 /pmc/articles/PMC7819154/ /pubmed/33472593 http://dx.doi.org/10.1186/s12864-020-07354-8 Text en © The Author(s) 2021 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research Article
Sakka, Maria K.
Riga, Maria
Ioannidis, Panagiotis
Baliota, Georgia V.
Tselika, Martha
Jagadeesan, Rajeswaran
Nayak, Manoj K.
Vontas, John
Athanassiou, Christos G.
Transcriptomic analysis of s-methoprene resistance in the lesser grain borer, Rhyzopertha dominica, and evaluation of piperonyl butoxide as a resistance breaker
title Transcriptomic analysis of s-methoprene resistance in the lesser grain borer, Rhyzopertha dominica, and evaluation of piperonyl butoxide as a resistance breaker
title_full Transcriptomic analysis of s-methoprene resistance in the lesser grain borer, Rhyzopertha dominica, and evaluation of piperonyl butoxide as a resistance breaker
title_fullStr Transcriptomic analysis of s-methoprene resistance in the lesser grain borer, Rhyzopertha dominica, and evaluation of piperonyl butoxide as a resistance breaker
title_full_unstemmed Transcriptomic analysis of s-methoprene resistance in the lesser grain borer, Rhyzopertha dominica, and evaluation of piperonyl butoxide as a resistance breaker
title_short Transcriptomic analysis of s-methoprene resistance in the lesser grain borer, Rhyzopertha dominica, and evaluation of piperonyl butoxide as a resistance breaker
title_sort transcriptomic analysis of s-methoprene resistance in the lesser grain borer, rhyzopertha dominica, and evaluation of piperonyl butoxide as a resistance breaker
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7819154/
https://www.ncbi.nlm.nih.gov/pubmed/33472593
http://dx.doi.org/10.1186/s12864-020-07354-8
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