Cargando…

Molecular evidence of the hybrid origin of Cryptocoryne ×purpurea Ridl. nothovar. purpurea (Araceae)

Natural hybridization has been considered a source of taxonomic complexity in Cryptocoryne. A combined study of DNA sequencing data from the internal transcribed spacer (ITS) of nuclear ribosomal DNA and the trnK-matK region of chloroplast DNA was used to identify the parents of Cryptocoryne putativ...

Descripción completa

Detalles Bibliográficos
Autores principales: Rosazlina, Rusly, Jacobsen, Niels, Ørgaard, Marian, Othman, Ahmad Sofiman
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7819605/
https://www.ncbi.nlm.nih.gov/pubmed/33476321
http://dx.doi.org/10.1371/journal.pone.0239499
_version_ 1783639036557000704
author Rosazlina, Rusly
Jacobsen, Niels
Ørgaard, Marian
Othman, Ahmad Sofiman
author_facet Rosazlina, Rusly
Jacobsen, Niels
Ørgaard, Marian
Othman, Ahmad Sofiman
author_sort Rosazlina, Rusly
collection PubMed
description Natural hybridization has been considered a source of taxonomic complexity in Cryptocoryne. A combined study of DNA sequencing data from the internal transcribed spacer (ITS) of nuclear ribosomal DNA and the trnK-matK region of chloroplast DNA was used to identify the parents of Cryptocoryne putative hybrids from Peninsular Malaysia. Based on the intermediate morphology and sympatric distribution area, the plants were tentatively identified as the hybrid Cryptocoryne ×purpurea nothovar. purpurea. The plants were pollen sterile and had long been considered as hybrids, supposedly between two related and co-existing species, C. cordata var. cordata and C. griffithii. The status of C. ×purpurea nothovar. purpurea was independently confirmed by the presence of an additive ITS sequence pattern from these two parental species in hybrid individuals. An analysis of the chloroplast trnK-matK sequences showed that the hybridization is bidirectional with the putative hybrids sharing identical sequences from C. cordata var. cordata and C. griffithii, indicating that both putative parental species had been the maternal parent in different accessions.
format Online
Article
Text
id pubmed-7819605
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-78196052021-01-28 Molecular evidence of the hybrid origin of Cryptocoryne ×purpurea Ridl. nothovar. purpurea (Araceae) Rosazlina, Rusly Jacobsen, Niels Ørgaard, Marian Othman, Ahmad Sofiman PLoS One Research Article Natural hybridization has been considered a source of taxonomic complexity in Cryptocoryne. A combined study of DNA sequencing data from the internal transcribed spacer (ITS) of nuclear ribosomal DNA and the trnK-matK region of chloroplast DNA was used to identify the parents of Cryptocoryne putative hybrids from Peninsular Malaysia. Based on the intermediate morphology and sympatric distribution area, the plants were tentatively identified as the hybrid Cryptocoryne ×purpurea nothovar. purpurea. The plants were pollen sterile and had long been considered as hybrids, supposedly between two related and co-existing species, C. cordata var. cordata and C. griffithii. The status of C. ×purpurea nothovar. purpurea was independently confirmed by the presence of an additive ITS sequence pattern from these two parental species in hybrid individuals. An analysis of the chloroplast trnK-matK sequences showed that the hybridization is bidirectional with the putative hybrids sharing identical sequences from C. cordata var. cordata and C. griffithii, indicating that both putative parental species had been the maternal parent in different accessions. Public Library of Science 2021-01-21 /pmc/articles/PMC7819605/ /pubmed/33476321 http://dx.doi.org/10.1371/journal.pone.0239499 Text en © 2021 Rosazlina et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Rosazlina, Rusly
Jacobsen, Niels
Ørgaard, Marian
Othman, Ahmad Sofiman
Molecular evidence of the hybrid origin of Cryptocoryne ×purpurea Ridl. nothovar. purpurea (Araceae)
title Molecular evidence of the hybrid origin of Cryptocoryne ×purpurea Ridl. nothovar. purpurea (Araceae)
title_full Molecular evidence of the hybrid origin of Cryptocoryne ×purpurea Ridl. nothovar. purpurea (Araceae)
title_fullStr Molecular evidence of the hybrid origin of Cryptocoryne ×purpurea Ridl. nothovar. purpurea (Araceae)
title_full_unstemmed Molecular evidence of the hybrid origin of Cryptocoryne ×purpurea Ridl. nothovar. purpurea (Araceae)
title_short Molecular evidence of the hybrid origin of Cryptocoryne ×purpurea Ridl. nothovar. purpurea (Araceae)
title_sort molecular evidence of the hybrid origin of cryptocoryne ×purpurea ridl. nothovar. purpurea (araceae)
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7819605/
https://www.ncbi.nlm.nih.gov/pubmed/33476321
http://dx.doi.org/10.1371/journal.pone.0239499
work_keys_str_mv AT rosazlinarusly molecularevidenceofthehybridoriginofcryptocorynepurpurearidlnothovarpurpureaaraceae
AT jacobsenniels molecularevidenceofthehybridoriginofcryptocorynepurpurearidlnothovarpurpureaaraceae
AT ørgaardmarian molecularevidenceofthehybridoriginofcryptocorynepurpurearidlnothovarpurpureaaraceae
AT othmanahmadsofiman molecularevidenceofthehybridoriginofcryptocorynepurpurearidlnothovarpurpureaaraceae