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Impact of Bacillus on Phthalides Accumulation in Angelica sinensis (Oliv.) by Stoichiometry and Microbial Diversity Analysis

Plant-microorganism interaction in the rhizosphere is thought to play an important role in the formation of soil fertility, transformation and absorption of nutrients, growth and development of medicinal plants, and accumulation of medicinal ingredients. Yet, the role that they play in the phthalide...

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Autores principales: Feng, Wei-Meng, Liu, Pei, Yan, Hui, Zhang, Sen, Shang, Er-Xin, Yu, Guang, Jiang, Shu, Qian, Da-Wei, Ma, Jun-Wei, Duan, Jin-Ao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7819887/
https://www.ncbi.nlm.nih.gov/pubmed/33488552
http://dx.doi.org/10.3389/fmicb.2020.611143
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author Feng, Wei-Meng
Liu, Pei
Yan, Hui
Zhang, Sen
Shang, Er-Xin
Yu, Guang
Jiang, Shu
Qian, Da-Wei
Ma, Jun-Wei
Duan, Jin-Ao
author_facet Feng, Wei-Meng
Liu, Pei
Yan, Hui
Zhang, Sen
Shang, Er-Xin
Yu, Guang
Jiang, Shu
Qian, Da-Wei
Ma, Jun-Wei
Duan, Jin-Ao
author_sort Feng, Wei-Meng
collection PubMed
description Plant-microorganism interaction in the rhizosphere is thought to play an important role in the formation of soil fertility, transformation and absorption of nutrients, growth and development of medicinal plants, and accumulation of medicinal ingredients. Yet, the role that they play in the phthalides accumulation of Angelica sinensis (Oliv.) Diels remains unclear. In the present study, we report a correlative analysis between rhizosphere microorganisms and phthalides accumulation in A. sinensis from Gansu, China where was the major production areas. Meanwhile, Bacillus was explored the potential functions in the plant growth and phthalide accumulation. Results revealed that the common bacterial species detected in six samples comprised 1150 OTUs which were involved in 368 genera, and predominant taxa include Actinobacteria, Acidobacteria, and Proteobacteria. The average contents of the six phthalides were 4.0329 mg/g. The correlation analysis indicated that 20 high abundance strains showed positive or negative correlations with phthalides accumulation. Flavobacterium, Nitrospira, Gaiella, Bradyrhizobium, Mycobacterium, Bacillus, RB41, Blastococcus, Nocardioides, and Solirubrobacter may be the key strains that affect phthalides accumulation on the genus level. By the plant-bacterial co-culture and fermentation, Bacillus which were isolated from rhizosphere soils can promote the plant growth, biomass accumulation and increased the contents of the butylidenephthalide (36∼415%) while the ligustilide (12∼67%) was decreased. Altogether, there is an interaction between rhizosphere microorganisms and phthalides accumulation in A. sinensis, Bacillus could promote butylidenephthalide accumulation while inhibiting ligustilide accumulation.
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spelling pubmed-78198872021-01-23 Impact of Bacillus on Phthalides Accumulation in Angelica sinensis (Oliv.) by Stoichiometry and Microbial Diversity Analysis Feng, Wei-Meng Liu, Pei Yan, Hui Zhang, Sen Shang, Er-Xin Yu, Guang Jiang, Shu Qian, Da-Wei Ma, Jun-Wei Duan, Jin-Ao Front Microbiol Microbiology Plant-microorganism interaction in the rhizosphere is thought to play an important role in the formation of soil fertility, transformation and absorption of nutrients, growth and development of medicinal plants, and accumulation of medicinal ingredients. Yet, the role that they play in the phthalides accumulation of Angelica sinensis (Oliv.) Diels remains unclear. In the present study, we report a correlative analysis between rhizosphere microorganisms and phthalides accumulation in A. sinensis from Gansu, China where was the major production areas. Meanwhile, Bacillus was explored the potential functions in the plant growth and phthalide accumulation. Results revealed that the common bacterial species detected in six samples comprised 1150 OTUs which were involved in 368 genera, and predominant taxa include Actinobacteria, Acidobacteria, and Proteobacteria. The average contents of the six phthalides were 4.0329 mg/g. The correlation analysis indicated that 20 high abundance strains showed positive or negative correlations with phthalides accumulation. Flavobacterium, Nitrospira, Gaiella, Bradyrhizobium, Mycobacterium, Bacillus, RB41, Blastococcus, Nocardioides, and Solirubrobacter may be the key strains that affect phthalides accumulation on the genus level. By the plant-bacterial co-culture and fermentation, Bacillus which were isolated from rhizosphere soils can promote the plant growth, biomass accumulation and increased the contents of the butylidenephthalide (36∼415%) while the ligustilide (12∼67%) was decreased. Altogether, there is an interaction between rhizosphere microorganisms and phthalides accumulation in A. sinensis, Bacillus could promote butylidenephthalide accumulation while inhibiting ligustilide accumulation. Frontiers Media S.A. 2021-01-08 /pmc/articles/PMC7819887/ /pubmed/33488552 http://dx.doi.org/10.3389/fmicb.2020.611143 Text en Copyright © 2021 Feng, Liu, Yan, Zhang, Shang, Yu, Jiang, Qian, Ma and Duan. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Feng, Wei-Meng
Liu, Pei
Yan, Hui
Zhang, Sen
Shang, Er-Xin
Yu, Guang
Jiang, Shu
Qian, Da-Wei
Ma, Jun-Wei
Duan, Jin-Ao
Impact of Bacillus on Phthalides Accumulation in Angelica sinensis (Oliv.) by Stoichiometry and Microbial Diversity Analysis
title Impact of Bacillus on Phthalides Accumulation in Angelica sinensis (Oliv.) by Stoichiometry and Microbial Diversity Analysis
title_full Impact of Bacillus on Phthalides Accumulation in Angelica sinensis (Oliv.) by Stoichiometry and Microbial Diversity Analysis
title_fullStr Impact of Bacillus on Phthalides Accumulation in Angelica sinensis (Oliv.) by Stoichiometry and Microbial Diversity Analysis
title_full_unstemmed Impact of Bacillus on Phthalides Accumulation in Angelica sinensis (Oliv.) by Stoichiometry and Microbial Diversity Analysis
title_short Impact of Bacillus on Phthalides Accumulation in Angelica sinensis (Oliv.) by Stoichiometry and Microbial Diversity Analysis
title_sort impact of bacillus on phthalides accumulation in angelica sinensis (oliv.) by stoichiometry and microbial diversity analysis
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7819887/
https://www.ncbi.nlm.nih.gov/pubmed/33488552
http://dx.doi.org/10.3389/fmicb.2020.611143
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