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Regenerative medicine for skeletal muscle loss: a review of current tissue engineering approaches

Skeletal muscle is capable of regeneration following minor damage, more significant volumetric muscle loss (VML) however results in permanent functional impairment. Current multimodal treatment methodologies yield variable functional recovery, with reconstructive surgical approaches restricted by li...

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Detalles Bibliográficos
Autores principales: Langridge, Benjamin, Griffin, Michelle, Butler, Peter E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer US 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7819922/
https://www.ncbi.nlm.nih.gov/pubmed/33475855
http://dx.doi.org/10.1007/s10856-020-06476-5
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author Langridge, Benjamin
Griffin, Michelle
Butler, Peter E.
author_facet Langridge, Benjamin
Griffin, Michelle
Butler, Peter E.
author_sort Langridge, Benjamin
collection PubMed
description Skeletal muscle is capable of regeneration following minor damage, more significant volumetric muscle loss (VML) however results in permanent functional impairment. Current multimodal treatment methodologies yield variable functional recovery, with reconstructive surgical approaches restricted by limited donor tissue and significant donor morbidity. Tissue-engineered skeletal muscle constructs promise the potential to revolutionise the treatment of VML through the regeneration of functional skeletal muscle. Herein, we review the current status of tissue engineering approaches to VML; firstly the design of biocompatible tissue scaffolds, including recent developments with electroconductive materials. Secondly, we review the progenitor cell populations used to seed scaffolds and their relative merits. Thirdly we review in vitro methods of scaffold functional maturation including the use of three-dimensional bioprinting and bioreactors. Finally, we discuss the technical, regulatory and ethical barriers to clinical translation of this technology. Despite significant advances in areas, such as electroactive scaffolds and three-dimensional bioprinting, along with several promising in vivo studies, there remain multiple technical hurdles before translation into clinically impactful therapies can be achieved. Novel strategies for graft vascularisation, and in vitro functional maturation will be of particular importance in order to develop tissue-engineered constructs capable of significant clinical impact.
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spelling pubmed-78199222021-01-28 Regenerative medicine for skeletal muscle loss: a review of current tissue engineering approaches Langridge, Benjamin Griffin, Michelle Butler, Peter E. J Mater Sci Mater Med Tissue Engineering Constructs and Cell Substrates Skeletal muscle is capable of regeneration following minor damage, more significant volumetric muscle loss (VML) however results in permanent functional impairment. Current multimodal treatment methodologies yield variable functional recovery, with reconstructive surgical approaches restricted by limited donor tissue and significant donor morbidity. Tissue-engineered skeletal muscle constructs promise the potential to revolutionise the treatment of VML through the regeneration of functional skeletal muscle. Herein, we review the current status of tissue engineering approaches to VML; firstly the design of biocompatible tissue scaffolds, including recent developments with electroconductive materials. Secondly, we review the progenitor cell populations used to seed scaffolds and their relative merits. Thirdly we review in vitro methods of scaffold functional maturation including the use of three-dimensional bioprinting and bioreactors. Finally, we discuss the technical, regulatory and ethical barriers to clinical translation of this technology. Despite significant advances in areas, such as electroactive scaffolds and three-dimensional bioprinting, along with several promising in vivo studies, there remain multiple technical hurdles before translation into clinically impactful therapies can be achieved. Novel strategies for graft vascularisation, and in vitro functional maturation will be of particular importance in order to develop tissue-engineered constructs capable of significant clinical impact. Springer US 2021-01-21 2021 /pmc/articles/PMC7819922/ /pubmed/33475855 http://dx.doi.org/10.1007/s10856-020-06476-5 Text en © The Author(s) 2021 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Tissue Engineering Constructs and Cell Substrates
Langridge, Benjamin
Griffin, Michelle
Butler, Peter E.
Regenerative medicine for skeletal muscle loss: a review of current tissue engineering approaches
title Regenerative medicine for skeletal muscle loss: a review of current tissue engineering approaches
title_full Regenerative medicine for skeletal muscle loss: a review of current tissue engineering approaches
title_fullStr Regenerative medicine for skeletal muscle loss: a review of current tissue engineering approaches
title_full_unstemmed Regenerative medicine for skeletal muscle loss: a review of current tissue engineering approaches
title_short Regenerative medicine for skeletal muscle loss: a review of current tissue engineering approaches
title_sort regenerative medicine for skeletal muscle loss: a review of current tissue engineering approaches
topic Tissue Engineering Constructs and Cell Substrates
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7819922/
https://www.ncbi.nlm.nih.gov/pubmed/33475855
http://dx.doi.org/10.1007/s10856-020-06476-5
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