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Lactobacillus attenuates progression of nonalcoholic fatty liver disease by lowering cholesterol and steatosis
BACKGROUND/AIMS: Nonalcoholic fatty liver disease (NAFLD) is closely related to gut-microbiome. There is a paucity of research on which strains of gut microbiota affect the progression of NAFLD. This study explored the NAFLD-associated microbiome in humans and the role of Lactobacillus in the progre...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Korean Association for the Study of the Liver
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7820205/ https://www.ncbi.nlm.nih.gov/pubmed/33317254 http://dx.doi.org/10.3350/cmh.2020.0125 |
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author | Lee, Na Young Shin, Min Jea Youn, Gi Soo Yoon, Sang Jun Choi, Ye Rin Kim, Hyeong Seop Gupta, Haripriya Han, Sang Hak Kim, Byoung Kook Lee, Do Yup Park, Tae Sik Sung, Hotaik Kim, Byung Yong Suk, Ki Tae |
author_facet | Lee, Na Young Shin, Min Jea Youn, Gi Soo Yoon, Sang Jun Choi, Ye Rin Kim, Hyeong Seop Gupta, Haripriya Han, Sang Hak Kim, Byoung Kook Lee, Do Yup Park, Tae Sik Sung, Hotaik Kim, Byung Yong Suk, Ki Tae |
author_sort | Lee, Na Young |
collection | PubMed |
description | BACKGROUND/AIMS: Nonalcoholic fatty liver disease (NAFLD) is closely related to gut-microbiome. There is a paucity of research on which strains of gut microbiota affect the progression of NAFLD. This study explored the NAFLD-associated microbiome in humans and the role of Lactobacillus in the progression of NAFLD in mice. METHODS: The gut microbiome was analyzed via next-generation sequencing in healthy people (n=37) and NAFLD patients with elevated liver enzymes (n=57). Six-week-old male C57BL/6J mice were separated into six groups (n=10 per group; normal, Western, and four Western diet + strains [10(9) colony-forming units/g for 8 weeks; L. acidophilus, L. fermentum, L. paracasei, and L. plantarum]). Liver/body weight ratio, liver pathology, serum analysis, and metagenomics in the mice were examined. RESULTS: Compared to healthy subjects (1.6±4.3), NAFLD patients showed an elevated Firmicutes/Bacteroidetes ratio (25.0±29.0) and a reduced composition of Akkermansia and L. murinus (P<0.05). In the animal experiment, L. acidophilus group was associated with a significant reduction in liver/body weight ratio (5.5±0.4) compared to the Western group (6.2±0.6) (P<0.05). L. acidophilus (41.0±8.6), L. fermentum (44.3±12.6), and L. plantarum (39.0±7.6) groups showed decreased cholesterol levels compared to the Western group (85.7±8.6) (P<0.05). In comparison of steatosis, L. acidophilus (1.9±0.6), L. plantarum (2.4±0.7), and L. paracasei (2.0±0.9) groups showed significant improvement of steatosis compared to the Western group (2.6±0.5) (P<0.05). CONCLUSIONS: Ingestion of Lactobacillus, such as L. acidophilus, L. fermentum, and L. plantarum, ameliorates the progression of nonalcoholic steatosis by lowering cholesterol. The use of Lactobacillus can be considered as a useful strategy for the treatment of NAFLD. |
format | Online Article Text |
id | pubmed-7820205 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | The Korean Association for the Study of the Liver |
record_format | MEDLINE/PubMed |
spelling | pubmed-78202052021-01-27 Lactobacillus attenuates progression of nonalcoholic fatty liver disease by lowering cholesterol and steatosis Lee, Na Young Shin, Min Jea Youn, Gi Soo Yoon, Sang Jun Choi, Ye Rin Kim, Hyeong Seop Gupta, Haripriya Han, Sang Hak Kim, Byoung Kook Lee, Do Yup Park, Tae Sik Sung, Hotaik Kim, Byung Yong Suk, Ki Tae Clin Mol Hepatol Original Article BACKGROUND/AIMS: Nonalcoholic fatty liver disease (NAFLD) is closely related to gut-microbiome. There is a paucity of research on which strains of gut microbiota affect the progression of NAFLD. This study explored the NAFLD-associated microbiome in humans and the role of Lactobacillus in the progression of NAFLD in mice. METHODS: The gut microbiome was analyzed via next-generation sequencing in healthy people (n=37) and NAFLD patients with elevated liver enzymes (n=57). Six-week-old male C57BL/6J mice were separated into six groups (n=10 per group; normal, Western, and four Western diet + strains [10(9) colony-forming units/g for 8 weeks; L. acidophilus, L. fermentum, L. paracasei, and L. plantarum]). Liver/body weight ratio, liver pathology, serum analysis, and metagenomics in the mice were examined. RESULTS: Compared to healthy subjects (1.6±4.3), NAFLD patients showed an elevated Firmicutes/Bacteroidetes ratio (25.0±29.0) and a reduced composition of Akkermansia and L. murinus (P<0.05). In the animal experiment, L. acidophilus group was associated with a significant reduction in liver/body weight ratio (5.5±0.4) compared to the Western group (6.2±0.6) (P<0.05). L. acidophilus (41.0±8.6), L. fermentum (44.3±12.6), and L. plantarum (39.0±7.6) groups showed decreased cholesterol levels compared to the Western group (85.7±8.6) (P<0.05). In comparison of steatosis, L. acidophilus (1.9±0.6), L. plantarum (2.4±0.7), and L. paracasei (2.0±0.9) groups showed significant improvement of steatosis compared to the Western group (2.6±0.5) (P<0.05). CONCLUSIONS: Ingestion of Lactobacillus, such as L. acidophilus, L. fermentum, and L. plantarum, ameliorates the progression of nonalcoholic steatosis by lowering cholesterol. The use of Lactobacillus can be considered as a useful strategy for the treatment of NAFLD. The Korean Association for the Study of the Liver 2021-01 2020-12-03 /pmc/articles/PMC7820205/ /pubmed/33317254 http://dx.doi.org/10.3350/cmh.2020.0125 Text en Copyright © 2021 by The Korean Association for the Study of the Liver This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Lee, Na Young Shin, Min Jea Youn, Gi Soo Yoon, Sang Jun Choi, Ye Rin Kim, Hyeong Seop Gupta, Haripriya Han, Sang Hak Kim, Byoung Kook Lee, Do Yup Park, Tae Sik Sung, Hotaik Kim, Byung Yong Suk, Ki Tae Lactobacillus attenuates progression of nonalcoholic fatty liver disease by lowering cholesterol and steatosis |
title | Lactobacillus attenuates progression of nonalcoholic fatty liver disease by lowering cholesterol and steatosis |
title_full | Lactobacillus attenuates progression of nonalcoholic fatty liver disease by lowering cholesterol and steatosis |
title_fullStr | Lactobacillus attenuates progression of nonalcoholic fatty liver disease by lowering cholesterol and steatosis |
title_full_unstemmed | Lactobacillus attenuates progression of nonalcoholic fatty liver disease by lowering cholesterol and steatosis |
title_short | Lactobacillus attenuates progression of nonalcoholic fatty liver disease by lowering cholesterol and steatosis |
title_sort | lactobacillus attenuates progression of nonalcoholic fatty liver disease by lowering cholesterol and steatosis |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7820205/ https://www.ncbi.nlm.nih.gov/pubmed/33317254 http://dx.doi.org/10.3350/cmh.2020.0125 |
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