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Cancer cell enrichment on a centrifugal microfluidic platform using hydrodynamic and magnetophoretic techniques

Isolation of rare cancer cells is one of the important and valuable stages of cancer research. Regarding the rarity of cancer cells in blood samples, it is important to invent an efficient separation device for cell enrichment. In this study, two centrifugal microfluidic devices were designed and fa...

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Autores principales: Shamloo, Amir, Naghdloo, Amin, Besanjideh, Mohsen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7820336/
https://www.ncbi.nlm.nih.gov/pubmed/33479404
http://dx.doi.org/10.1038/s41598-021-81661-2
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author Shamloo, Amir
Naghdloo, Amin
Besanjideh, Mohsen
author_facet Shamloo, Amir
Naghdloo, Amin
Besanjideh, Mohsen
author_sort Shamloo, Amir
collection PubMed
description Isolation of rare cancer cells is one of the important and valuable stages of cancer research. Regarding the rarity of cancer cells in blood samples, it is important to invent an efficient separation device for cell enrichment. In this study, two centrifugal microfluidic devices were designed and fabricated for the isolation of rare cancer cells. The first design (passive plan) employs a contraction–expansion array (CEA) microchannel which is connected to a bifurcation region. This device is able to isolate the target cells through inertial effects and bifurcation law. The second design (hybrid plan) also utilizes a CEA microchannel, but instead of using the bifurcation region, it is reinforced by a stack of two permanent magnets to capture the magnetically labeled target cells at the end of the microchannel. These designs were optimized by numerical simulations and tested experimentally for isolation of MCF-7 human breast cancer cells from the population of mouse fibroblast L929 cells. In order to use the hybrid design, magnetite nanoparticles were attached to the MCF-7 cells through specific Ep-CAM antibodies, and two permanent magnets of 0.34 T were utilized at the downstream of the CEA microchannel. These devices were tested at different disk rotational speeds and it was found that the passive design can isolate MCF-7 cells with a recovery rate of 76% for the rotational speed of 2100 rpm while its hybrid counterpart is able to separate the target cells with a recovery rate of 85% for the rotational speed of 1200 rpm. Although the hybrid design of separator has a better separation efficiency and higher purity, the passive one has no need for a time-consuming process of cell labeling, occupies less space on the disk, and does not impose additional costs and complexity.
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spelling pubmed-78203362021-01-22 Cancer cell enrichment on a centrifugal microfluidic platform using hydrodynamic and magnetophoretic techniques Shamloo, Amir Naghdloo, Amin Besanjideh, Mohsen Sci Rep Article Isolation of rare cancer cells is one of the important and valuable stages of cancer research. Regarding the rarity of cancer cells in blood samples, it is important to invent an efficient separation device for cell enrichment. In this study, two centrifugal microfluidic devices were designed and fabricated for the isolation of rare cancer cells. The first design (passive plan) employs a contraction–expansion array (CEA) microchannel which is connected to a bifurcation region. This device is able to isolate the target cells through inertial effects and bifurcation law. The second design (hybrid plan) also utilizes a CEA microchannel, but instead of using the bifurcation region, it is reinforced by a stack of two permanent magnets to capture the magnetically labeled target cells at the end of the microchannel. These designs were optimized by numerical simulations and tested experimentally for isolation of MCF-7 human breast cancer cells from the population of mouse fibroblast L929 cells. In order to use the hybrid design, magnetite nanoparticles were attached to the MCF-7 cells through specific Ep-CAM antibodies, and two permanent magnets of 0.34 T were utilized at the downstream of the CEA microchannel. These devices were tested at different disk rotational speeds and it was found that the passive design can isolate MCF-7 cells with a recovery rate of 76% for the rotational speed of 2100 rpm while its hybrid counterpart is able to separate the target cells with a recovery rate of 85% for the rotational speed of 1200 rpm. Although the hybrid design of separator has a better separation efficiency and higher purity, the passive one has no need for a time-consuming process of cell labeling, occupies less space on the disk, and does not impose additional costs and complexity. Nature Publishing Group UK 2021-01-21 /pmc/articles/PMC7820336/ /pubmed/33479404 http://dx.doi.org/10.1038/s41598-021-81661-2 Text en © The Author(s) 2021 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Shamloo, Amir
Naghdloo, Amin
Besanjideh, Mohsen
Cancer cell enrichment on a centrifugal microfluidic platform using hydrodynamic and magnetophoretic techniques
title Cancer cell enrichment on a centrifugal microfluidic platform using hydrodynamic and magnetophoretic techniques
title_full Cancer cell enrichment on a centrifugal microfluidic platform using hydrodynamic and magnetophoretic techniques
title_fullStr Cancer cell enrichment on a centrifugal microfluidic platform using hydrodynamic and magnetophoretic techniques
title_full_unstemmed Cancer cell enrichment on a centrifugal microfluidic platform using hydrodynamic and magnetophoretic techniques
title_short Cancer cell enrichment on a centrifugal microfluidic platform using hydrodynamic and magnetophoretic techniques
title_sort cancer cell enrichment on a centrifugal microfluidic platform using hydrodynamic and magnetophoretic techniques
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7820336/
https://www.ncbi.nlm.nih.gov/pubmed/33479404
http://dx.doi.org/10.1038/s41598-021-81661-2
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