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3D high-density microelectrode array with optical stimulation and drug delivery for investigating neural circuit dynamics
Investigation of neural circuit dynamics is crucial for deciphering the functional connections among regions of the brain and understanding the mechanism of brain dysfunction. Despite the advancements of neural circuit models in vitro, technologies for both precisely monitoring and modulating neural...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7820464/ https://www.ncbi.nlm.nih.gov/pubmed/33479237 http://dx.doi.org/10.1038/s41467-020-20763-3 |
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author | Shin, Hyogeun Jeong, Sohyeon Lee, Ju-Hyun Sun, Woong Choi, Nakwon Cho, Il-Joo |
author_facet | Shin, Hyogeun Jeong, Sohyeon Lee, Ju-Hyun Sun, Woong Choi, Nakwon Cho, Il-Joo |
author_sort | Shin, Hyogeun |
collection | PubMed |
description | Investigation of neural circuit dynamics is crucial for deciphering the functional connections among regions of the brain and understanding the mechanism of brain dysfunction. Despite the advancements of neural circuit models in vitro, technologies for both precisely monitoring and modulating neural activities within three-dimensional (3D) neural circuit models have yet to be developed. Specifically, no existing 3D microelectrode arrays (MEAs) have integrated capabilities to stimulate surrounding neurons and to monitor the temporal evolution of the formation of a neural network in real time. Herein, we present a 3D high-density multifunctional MEA with optical stimulation and drug delivery for investigating neural circuit dynamics within engineered 3D neural tissues. We demonstrate precise measurements of synaptic latencies in 3D neural networks. We expect our 3D multifunctional MEA to open up opportunities for studies of neural circuits through precise, in vitro investigations of neural circuit dynamics with 3D brain models. |
format | Online Article Text |
id | pubmed-7820464 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-78204642021-01-29 3D high-density microelectrode array with optical stimulation and drug delivery for investigating neural circuit dynamics Shin, Hyogeun Jeong, Sohyeon Lee, Ju-Hyun Sun, Woong Choi, Nakwon Cho, Il-Joo Nat Commun Article Investigation of neural circuit dynamics is crucial for deciphering the functional connections among regions of the brain and understanding the mechanism of brain dysfunction. Despite the advancements of neural circuit models in vitro, technologies for both precisely monitoring and modulating neural activities within three-dimensional (3D) neural circuit models have yet to be developed. Specifically, no existing 3D microelectrode arrays (MEAs) have integrated capabilities to stimulate surrounding neurons and to monitor the temporal evolution of the formation of a neural network in real time. Herein, we present a 3D high-density multifunctional MEA with optical stimulation and drug delivery for investigating neural circuit dynamics within engineered 3D neural tissues. We demonstrate precise measurements of synaptic latencies in 3D neural networks. We expect our 3D multifunctional MEA to open up opportunities for studies of neural circuits through precise, in vitro investigations of neural circuit dynamics with 3D brain models. Nature Publishing Group UK 2021-01-21 /pmc/articles/PMC7820464/ /pubmed/33479237 http://dx.doi.org/10.1038/s41467-020-20763-3 Text en © The Author(s) 2021 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Shin, Hyogeun Jeong, Sohyeon Lee, Ju-Hyun Sun, Woong Choi, Nakwon Cho, Il-Joo 3D high-density microelectrode array with optical stimulation and drug delivery for investigating neural circuit dynamics |
title | 3D high-density microelectrode array with optical stimulation and drug delivery for investigating neural circuit dynamics |
title_full | 3D high-density microelectrode array with optical stimulation and drug delivery for investigating neural circuit dynamics |
title_fullStr | 3D high-density microelectrode array with optical stimulation and drug delivery for investigating neural circuit dynamics |
title_full_unstemmed | 3D high-density microelectrode array with optical stimulation and drug delivery for investigating neural circuit dynamics |
title_short | 3D high-density microelectrode array with optical stimulation and drug delivery for investigating neural circuit dynamics |
title_sort | 3d high-density microelectrode array with optical stimulation and drug delivery for investigating neural circuit dynamics |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7820464/ https://www.ncbi.nlm.nih.gov/pubmed/33479237 http://dx.doi.org/10.1038/s41467-020-20763-3 |
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