Repeat DNA-PAINT suppresses background and non-specific signals in optical nanoscopy

DNA-PAINT is a versatile optical super-resolution technique relying on the transient binding of fluorescent DNA ‘imagers’ to target epitopes. Its performance in biological samples is often constrained by strong background signals and non-specific binding events, both exacerbated by high imager conce...

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Autores principales: Clowsley, Alexander H., Kaufhold, William T., Lutz, Tobias, Meletiou, Anna, Di Michele, Lorenzo, Soeller, Christian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7820506/
https://www.ncbi.nlm.nih.gov/pubmed/33479249
http://dx.doi.org/10.1038/s41467-020-20686-z
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author Clowsley, Alexander H.
Kaufhold, William T.
Lutz, Tobias
Meletiou, Anna
Di Michele, Lorenzo
Soeller, Christian
author_facet Clowsley, Alexander H.
Kaufhold, William T.
Lutz, Tobias
Meletiou, Anna
Di Michele, Lorenzo
Soeller, Christian
author_sort Clowsley, Alexander H.
collection PubMed
description DNA-PAINT is a versatile optical super-resolution technique relying on the transient binding of fluorescent DNA ‘imagers’ to target epitopes. Its performance in biological samples is often constrained by strong background signals and non-specific binding events, both exacerbated by high imager concentrations. Here we describe Repeat DNA-PAINT, a method that enables a substantial reduction in imager concentration, thus suppressing spurious signals. Additionally, Repeat DNA-PAINT reduces photoinduced target-site loss and can accelerate sampling, all without affecting spatial resolution.
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spelling pubmed-78205062021-01-29 Repeat DNA-PAINT suppresses background and non-specific signals in optical nanoscopy Clowsley, Alexander H. Kaufhold, William T. Lutz, Tobias Meletiou, Anna Di Michele, Lorenzo Soeller, Christian Nat Commun Article DNA-PAINT is a versatile optical super-resolution technique relying on the transient binding of fluorescent DNA ‘imagers’ to target epitopes. Its performance in biological samples is often constrained by strong background signals and non-specific binding events, both exacerbated by high imager concentrations. Here we describe Repeat DNA-PAINT, a method that enables a substantial reduction in imager concentration, thus suppressing spurious signals. Additionally, Repeat DNA-PAINT reduces photoinduced target-site loss and can accelerate sampling, all without affecting spatial resolution. Nature Publishing Group UK 2021-01-21 /pmc/articles/PMC7820506/ /pubmed/33479249 http://dx.doi.org/10.1038/s41467-020-20686-z Text en © The Author(s) 2021 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Clowsley, Alexander H.
Kaufhold, William T.
Lutz, Tobias
Meletiou, Anna
Di Michele, Lorenzo
Soeller, Christian
Repeat DNA-PAINT suppresses background and non-specific signals in optical nanoscopy
title Repeat DNA-PAINT suppresses background and non-specific signals in optical nanoscopy
title_full Repeat DNA-PAINT suppresses background and non-specific signals in optical nanoscopy
title_fullStr Repeat DNA-PAINT suppresses background and non-specific signals in optical nanoscopy
title_full_unstemmed Repeat DNA-PAINT suppresses background and non-specific signals in optical nanoscopy
title_short Repeat DNA-PAINT suppresses background and non-specific signals in optical nanoscopy
title_sort repeat dna-paint suppresses background and non-specific signals in optical nanoscopy
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7820506/
https://www.ncbi.nlm.nih.gov/pubmed/33479249
http://dx.doi.org/10.1038/s41467-020-20686-z
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