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Preparation, Scanning and Analysis of Duckweed Using X-Ray Computed Microtomography
Quantification of anatomical and compositional features underpins both fundamental and applied studies of plant structure and function. Relatively few non-invasive techniques are available for aquatic plants. Traditional methods such as sectioning are low-throughput and provide 2-dimensional informa...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7820725/ https://www.ncbi.nlm.nih.gov/pubmed/33488660 http://dx.doi.org/10.3389/fpls.2020.617830 |
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author | Jones, Dylan H. Atkinson, Brian S. Ware, Alexander Sturrock, Craig J. Bishopp, Anthony Wells, Darren M. |
author_facet | Jones, Dylan H. Atkinson, Brian S. Ware, Alexander Sturrock, Craig J. Bishopp, Anthony Wells, Darren M. |
author_sort | Jones, Dylan H. |
collection | PubMed |
description | Quantification of anatomical and compositional features underpins both fundamental and applied studies of plant structure and function. Relatively few non-invasive techniques are available for aquatic plants. Traditional methods such as sectioning are low-throughput and provide 2-dimensional information. X-ray Computed Microtomography (μCT) offers a non-destructive method of three dimensional (3D) imaging in planta, but has not been widely used for aquatic species, due to the difficulties in sample preparation and handling. We present a novel sample handling protocol for aquatic plant material developed for μCT imaging, using duckweed plants and turions as exemplars, and compare the method against existing approaches. This technique allows for previously unseen 3D volume analysis of gaseous filled spaces, cell material, and sub-cellular features. The described embedding method, utilizing petrolatum gel for sample mounting, was shown to preserve sample quality during scanning, and to display sufficiently different X-ray attenuation to the plant material to be easily differentiated by image analysis pipelines. We present this technique as an improved method for anatomical structural analysis that provides novel cellular and developmental information. |
format | Online Article Text |
id | pubmed-7820725 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-78207252021-01-23 Preparation, Scanning and Analysis of Duckweed Using X-Ray Computed Microtomography Jones, Dylan H. Atkinson, Brian S. Ware, Alexander Sturrock, Craig J. Bishopp, Anthony Wells, Darren M. Front Plant Sci Plant Science Quantification of anatomical and compositional features underpins both fundamental and applied studies of plant structure and function. Relatively few non-invasive techniques are available for aquatic plants. Traditional methods such as sectioning are low-throughput and provide 2-dimensional information. X-ray Computed Microtomography (μCT) offers a non-destructive method of three dimensional (3D) imaging in planta, but has not been widely used for aquatic species, due to the difficulties in sample preparation and handling. We present a novel sample handling protocol for aquatic plant material developed for μCT imaging, using duckweed plants and turions as exemplars, and compare the method against existing approaches. This technique allows for previously unseen 3D volume analysis of gaseous filled spaces, cell material, and sub-cellular features. The described embedding method, utilizing petrolatum gel for sample mounting, was shown to preserve sample quality during scanning, and to display sufficiently different X-ray attenuation to the plant material to be easily differentiated by image analysis pipelines. We present this technique as an improved method for anatomical structural analysis that provides novel cellular and developmental information. Frontiers Media S.A. 2021-01-08 /pmc/articles/PMC7820725/ /pubmed/33488660 http://dx.doi.org/10.3389/fpls.2020.617830 Text en Copyright © 2021 Jones, Atkinson, Ware, Sturrock, Bishopp and Wells. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Plant Science Jones, Dylan H. Atkinson, Brian S. Ware, Alexander Sturrock, Craig J. Bishopp, Anthony Wells, Darren M. Preparation, Scanning and Analysis of Duckweed Using X-Ray Computed Microtomography |
title | Preparation, Scanning and Analysis of Duckweed Using X-Ray Computed Microtomography |
title_full | Preparation, Scanning and Analysis of Duckweed Using X-Ray Computed Microtomography |
title_fullStr | Preparation, Scanning and Analysis of Duckweed Using X-Ray Computed Microtomography |
title_full_unstemmed | Preparation, Scanning and Analysis of Duckweed Using X-Ray Computed Microtomography |
title_short | Preparation, Scanning and Analysis of Duckweed Using X-Ray Computed Microtomography |
title_sort | preparation, scanning and analysis of duckweed using x-ray computed microtomography |
topic | Plant Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7820725/ https://www.ncbi.nlm.nih.gov/pubmed/33488660 http://dx.doi.org/10.3389/fpls.2020.617830 |
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