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Single cell RNA-sequencing data generated from human pluripotent stem cell-derived lens epithelial cells
Detailed transcriptomic analyses of differentiated cell populations derived from human pluripotent stem cells is routinely used to assess the identity and utility of the differentiated cells. Here we provide single cell RNA-sequencing data obtained from ROR1-expressing lens epithelial cells (ROR1e L...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7820909/ https://www.ncbi.nlm.nih.gov/pubmed/33521174 http://dx.doi.org/10.1016/j.dib.2020.106657 |
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author | Shparberg, Rachel Dewi, Chitra Umala Gnanasambandapillai, Vikkitharan Liyanage, Liwan O'Connor, Michael D. |
author_facet | Shparberg, Rachel Dewi, Chitra Umala Gnanasambandapillai, Vikkitharan Liyanage, Liwan O'Connor, Michael D. |
author_sort | Shparberg, Rachel |
collection | PubMed |
description | Detailed transcriptomic analyses of differentiated cell populations derived from human pluripotent stem cells is routinely used to assess the identity and utility of the differentiated cells. Here we provide single cell RNA-sequencing data obtained from ROR1-expressing lens epithelial cells (ROR1e LECs), obtained via directed differentiation of CA1 human embryonic stem cells. Analysis of the data using principal component analysis, heat maps and gene ontology assessments revealed phenotypes associated with lens epithelial cells. These data provide a resource for future characterisation of both normal and cataractous human lens biology. Corresponding morphological and functional data obtained from ROR1e LECs are reported in the associated research article “A simplified method for producing human lens epithelial cells and light-focusing micro-lenses from pluripotent stem cells “ (Dewi et al., 2020). |
format | Online Article Text |
id | pubmed-7820909 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-78209092021-01-29 Single cell RNA-sequencing data generated from human pluripotent stem cell-derived lens epithelial cells Shparberg, Rachel Dewi, Chitra Umala Gnanasambandapillai, Vikkitharan Liyanage, Liwan O'Connor, Michael D. Data Brief Data Article Detailed transcriptomic analyses of differentiated cell populations derived from human pluripotent stem cells is routinely used to assess the identity and utility of the differentiated cells. Here we provide single cell RNA-sequencing data obtained from ROR1-expressing lens epithelial cells (ROR1e LECs), obtained via directed differentiation of CA1 human embryonic stem cells. Analysis of the data using principal component analysis, heat maps and gene ontology assessments revealed phenotypes associated with lens epithelial cells. These data provide a resource for future characterisation of both normal and cataractous human lens biology. Corresponding morphological and functional data obtained from ROR1e LECs are reported in the associated research article “A simplified method for producing human lens epithelial cells and light-focusing micro-lenses from pluripotent stem cells “ (Dewi et al., 2020). Elsevier 2021-01-08 /pmc/articles/PMC7820909/ /pubmed/33521174 http://dx.doi.org/10.1016/j.dib.2020.106657 Text en © 2021 The Authors http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Data Article Shparberg, Rachel Dewi, Chitra Umala Gnanasambandapillai, Vikkitharan Liyanage, Liwan O'Connor, Michael D. Single cell RNA-sequencing data generated from human pluripotent stem cell-derived lens epithelial cells |
title | Single cell RNA-sequencing data generated from human pluripotent stem cell-derived lens epithelial cells |
title_full | Single cell RNA-sequencing data generated from human pluripotent stem cell-derived lens epithelial cells |
title_fullStr | Single cell RNA-sequencing data generated from human pluripotent stem cell-derived lens epithelial cells |
title_full_unstemmed | Single cell RNA-sequencing data generated from human pluripotent stem cell-derived lens epithelial cells |
title_short | Single cell RNA-sequencing data generated from human pluripotent stem cell-derived lens epithelial cells |
title_sort | single cell rna-sequencing data generated from human pluripotent stem cell-derived lens epithelial cells |
topic | Data Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7820909/ https://www.ncbi.nlm.nih.gov/pubmed/33521174 http://dx.doi.org/10.1016/j.dib.2020.106657 |
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