Cargando…

Type of culture medium determines properties of cultivated retinal endothelial cells: induction of substantial phenotypic conversion by standard DMEM

Contradictory behavior of microvascular retinal endothelial cells (REC) - a reliable in vitro model to study retinal diseases - have recently been reported which might result from cultivating the cells in standard DMEM not optimized for this cell type. Therefore, we studied DMEM's effects on ph...

Descripción completa

Detalles Bibliográficos
Autores principales:	Busch, Catharina, Rehak, Matus, Hollborn, Margrit, Wiedemann, Peter, Lang, Gerhard K., Lang, Gabriele E., Wolf, Armin, Deissler, Heidrun L.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2021
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7820930/ https://www.ncbi.nlm.nih.gov/pubmed/33521368 http://dx.doi.org/10.1016/j.heliyon.2021.e06037

Ejemplares similares

Different responses of the MIO‑M1 Mueller cell line to angiotensin II under hyperglycemic or hypoxic conditions
por: Beuse, Ansgar, et al.
Publicado: (2023)

Beovu, but not Lucentis impairs the function of the barrier formed by retinal endothelial cells in vitro
por: Deissler, Heidrun L., et al.
Publicado: (2022)

Impairment of the Retinal Endothelial Cell Barrier Induced by Long-Term Treatment with VEGF-A(165) No Longer Depends on the Growth Factor’s Presence
por: Deissler, Heidrun L., et al.
Publicado: (2022)

Osmotic and hypoxic induction of osteopontin in retinal pigment epithelial cells: Involvement of purinergic receptor signaling
por: Hollborn, Margrit, et al.
Publicado: (2020)

Osmotic and hypoxic induction of the complement factor C9 in cultured human retinal pigment epithelial cells: Regulation of VEGF and NLRP3 expression
por: Hollborn, Margrit, et al.
Publicado: (2018)

Comparison of Rat Primary Midbrain Neurons Cultured in DMEM/F12 and Neurobasal Mediums
por: Valian, Neda, et al.
Publicado: (2021)

Osmotic regulation of aquaporin-8 expression in retinal pigment epithelial cells in vitro: Dependence on K(ATP) channel activation
por: Schnabel, Benjamin, et al.
Publicado: (2020)

Osmotic induction of cyclooxygenase-2 in RPE cells: Stimulation of inflammasome activation
por: Messerschmidt, Luise, et al.
Publicado: (2019)

Ranibizumab efficiently blocks migration but not proliferation induced by growth factor combinations including VEGF in retinal endothelial cells
por: Deissler, Heidrun L., et al.
Publicado: (2013)

Gene expression regulation in retinal pigment epithelial cells induced by viral RNA and viral/bacterial DNA
por: Brosig, Anton, et al.
Publicado: (2015)

Osmotic regulation of NFAT5 expression in RPE cells: The involvement of purinergic receptor signaling
por: Hollborn, Margrit, et al.
Publicado: (2017)

Osmotic Induction of Angiogenic Growth Factor Expression in Human Retinal Pigment Epithelial Cells
por: Veltmann, Moritz, et al.
Publicado: (2016)

Regulation of the hyperosmotic induction of aquaporin 5 and VEGF in retinal pigment epithelial cells: Involvement of NFAT5
por: Hollborn, Margrit, et al.
Publicado: (2015)

Kir4.2 Potassium Channels in Retinal Pigment Epithelial Cells In Vitro: Contribution to Cell Viability and Proliferation, and Down-Regulation by Vascular Endothelial Growth Factor
por: Beer, Marie-Christin, et al.
Publicado: (2022)

Binding of VEGF-A is sufficient to abrogate the disturbing effects of VEGF-B together with VEGF-A on retinal endothelial cells
por: Deissler, Heidrun L., et al.
Publicado: (2015)

Fate of the Fc fusion protein aflibercept in retinal endothelial cells: competition of recycling and degradation
por: Deissler, Heidrun L., et al.
Publicado: (2018)

Outcomes in diabetic macular edema switched directly or after a dexamethasone implant to a fluocinolone acetonide intravitreal implant following anti-VEGF treatment
por: Rehak, Matus, et al.
Publicado: (2019)

Intake of dietary salt and drinking water: Implications for the development of age-related macular degeneration
por: Bringmann, Andreas, et al.
Publicado: (2016)

Activator protein-1 contributes to the NaCl-induced expression of VEGF and PlGF in RPE cells
por: Kleiner, Jana, et al.
Publicado: (2018)

Actions of bevacizumab and ranibizumab on microvascular retinal endothelial cells: similarities and differences
por: Deissler, Heidrun L, et al.
Publicado: (2012)

Medium-sized deletion in the BRCA1 gene: Limitations of Sanger sequencing and MLPA analyses
por: Herman, Sandra, et al.
Publicado: (2012)

KAv-1 is Better Suited to Chick Fibroblast Culture than DMEM or 199 Media
por: Katayama, Masafumi, et al.
Publicado: (2021)

Sitagliptin and the Blood-Retina Barrier: Effects on Retinal Endothelial Cells Manifested Only after Prolonged Exposure
por: Jäckle, Anja, et al.
Publicado: (2020)

Cytotoxic Effects of Curcumin in Human Retinal Pigment Epithelial Cells
por: Hollborn, Margrit, et al.
Publicado: (2013)

P2Y(1) Receptor Signaling Contributes to High Salt-Induced Priming of the NLRP3 Inflammasome in Retinal Pigment Epithelial Cells
por: Prager, Philipp, et al.
Publicado: (2016)

General health of patients with diabetic macular edema—The LIPSIA study
por: Busch, Catharina, et al.
Publicado: (2021)

Effects of the vegetable polyphenols epigallocatechin-3-gallate, luteolin, apigenin, myricetin, quercetin, and cyanidin in primary cultures of human retinal pigment epithelial cells
por: Chen, Rui, et al.
Publicado: (2014)

Müller Cell Reactivity in Response to Photoreceptor Degeneration in Rats with Defective Polycystin-2
por: Vogler, Stefanie, et al.
Publicado: (2013)

A Liquid Chromatography with Tandem Mass Spectrometry-Based Proteomic Analysis of Cells Cultured in DMEM 10% FBS and Chemically Defined Medium Using Human Adipose-Derived Mesenchymal Stem Cells
por: Nakashima, Yoshiki, et al.
Publicado: (2018)

Responses of Solid Tumor Cells in DMEM to Reactive Oxygen Species Generated by Non-Thermal Plasma and Chemically Induced ROS Systems
por: Kaushik, Neha, et al.
Publicado: (2015)

Comparing the efficacy of trabeculectomy and XEN gel microstent implantation for the treatment of primary open-angle glaucoma: a retrospective monocentric comparative cohort study
por: Theilig, Theresa, et al.
Publicado: (2020)

Outcome of a single XEN microstent implant for glaucoma patients with different types of glaucoma
por: Schargus, Marc, et al.
Publicado: (2020)

Assessing the protective effects of different surface coatings on NaYF(4):Yb(3+), Er(3+) upconverting nanoparticles in buffer and DMEM
por: Saleh, Maysoon I., et al.
Publicado: (2020)

Morphology of partial-thickness macular defects: presumed roles of Müller cells and tissue layer interfaces of low mechanical stability
por: Bringmann, Andreas, et al.
Publicado: (2020)

Comparison of chromosomal instability of human amniocytes in primary and long-term cultures in AmnioMAX II and DMEM media: A cross-sectional study
por: Hoseini, Seyed Mehdi, et al.
Publicado: (2020)

The epidemiology of uveal melanoma in Germany: a nationwide report of incidence and survival between 2009 and 2015
por: Alfaar, Ahmad Samir, et al.
Publicado: (2021)

THE CULTIVATION OF MONOCYTES IN FLUID MEDIUM
por: Baker, Lillian E.
Publicado: (1933)

Functional Monitoring after Trabeculectomy or XEN Microstent Implantation Using Spectral Domain Optical Coherence Tomography and Visual Field Indices—A Retrospective Comparative Cohort Study
por: Schargus, Marc, et al.
Publicado: (2021)

Uveal melanoma and marital status: a relationship that affects survival
por: Alfaar, Ahmad, et al.
Publicado: (2022)

Two Year Functional and Structural Changes—A Comparison between Trabeculectomy and XEN Microstent Implantation Using Spectral Domain Optical Coherence Tomography
por: Bormann, Caroline, et al.
Publicado: (2022)

Cannot write session to /tmp/vufind_sessions/sess_gvumkl13kbred19rfb98up755o