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OsSYL2 (AA), an allele identified by gene‐based association, increases style length in rice (Oryza sativa L.)

Stigma characteristics are important factors affecting the seed yield of hybrid rice per unit area. Natural variation of stigma characteristics has been reported in rice, but the genetic basis for this variation is largely unknown. We performed a genome‐wide association study on three stigma charact...

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Autores principales: Dang, Xiaojing, Yang, Yang, Zhang, Yuanqing, Chen, Xiangong, Fan, Zhilan, Liu, Qiangming, Ji, Jie, Li, Dalu, Li, Yanhui, Fang, Bingjie, Wu, Zexu, Liu, Erbao, Hu, Xiaoxiao, Zhu, Shangshang, She, Dong, Wang, Hui, Li, Yulong, Chen, Siqi, Wu, Yufeng, Hong, Delin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7821000/
https://www.ncbi.nlm.nih.gov/pubmed/33031564
http://dx.doi.org/10.1111/tpj.15013
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author Dang, Xiaojing
Yang, Yang
Zhang, Yuanqing
Chen, Xiangong
Fan, Zhilan
Liu, Qiangming
Ji, Jie
Li, Dalu
Li, Yanhui
Fang, Bingjie
Wu, Zexu
Liu, Erbao
Hu, Xiaoxiao
Zhu, Shangshang
She, Dong
Wang, Hui
Li, Yulong
Chen, Siqi
Wu, Yufeng
Hong, Delin
author_facet Dang, Xiaojing
Yang, Yang
Zhang, Yuanqing
Chen, Xiangong
Fan, Zhilan
Liu, Qiangming
Ji, Jie
Li, Dalu
Li, Yanhui
Fang, Bingjie
Wu, Zexu
Liu, Erbao
Hu, Xiaoxiao
Zhu, Shangshang
She, Dong
Wang, Hui
Li, Yulong
Chen, Siqi
Wu, Yufeng
Hong, Delin
author_sort Dang, Xiaojing
collection PubMed
description Stigma characteristics are important factors affecting the seed yield of hybrid rice per unit area. Natural variation of stigma characteristics has been reported in rice, but the genetic basis for this variation is largely unknown. We performed a genome‐wide association study on three stigma characteristics in six environments using 1.3 million single‐nucleotide polymorphism (SNPs) characterized in 353 diverse accessions of Oryza sativa. An abundance of phenotypic variation was present in the three stigma characteristics of these collections. We identified four significant SNPs associated with stigma length, 20 SNPs with style length (SYL), and 17 SNPs with the sum of stigma and style length, which were detected repeatedly in more than four environments. Of these SNPs, 28 were novel. We identified two causal gene loci for SYL, OsSYL3 and OsSYL2; OsSYL3 was co‐localized with the grain size gene GS3. The SYL of accessions carrying allele OsSYL3(AA) was significantly longer than that of those carrying allele OsSYL3(CC). We also demonstrated that the outcrossing rate of female parents carrying allele OsSYL2(AA) increased by 5.71% compared with that of the isogenic line carrying allele OsSYL2(CC) in an F(1) hybrid seed production field. The allele frequencies of OsSYL3(AA) and OsSYL2(AA) decreased gradually with an increase in latitude in the Northern Hemisphere. Our results should facilitate the improvement in stigma characteristics of parents of hybrid rice.
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spelling pubmed-78210002021-01-26 OsSYL2 (AA), an allele identified by gene‐based association, increases style length in rice (Oryza sativa L.) Dang, Xiaojing Yang, Yang Zhang, Yuanqing Chen, Xiangong Fan, Zhilan Liu, Qiangming Ji, Jie Li, Dalu Li, Yanhui Fang, Bingjie Wu, Zexu Liu, Erbao Hu, Xiaoxiao Zhu, Shangshang She, Dong Wang, Hui Li, Yulong Chen, Siqi Wu, Yufeng Hong, Delin Plant J Original Articles Stigma characteristics are important factors affecting the seed yield of hybrid rice per unit area. Natural variation of stigma characteristics has been reported in rice, but the genetic basis for this variation is largely unknown. We performed a genome‐wide association study on three stigma characteristics in six environments using 1.3 million single‐nucleotide polymorphism (SNPs) characterized in 353 diverse accessions of Oryza sativa. An abundance of phenotypic variation was present in the three stigma characteristics of these collections. We identified four significant SNPs associated with stigma length, 20 SNPs with style length (SYL), and 17 SNPs with the sum of stigma and style length, which were detected repeatedly in more than four environments. Of these SNPs, 28 were novel. We identified two causal gene loci for SYL, OsSYL3 and OsSYL2; OsSYL3 was co‐localized with the grain size gene GS3. The SYL of accessions carrying allele OsSYL3(AA) was significantly longer than that of those carrying allele OsSYL3(CC). We also demonstrated that the outcrossing rate of female parents carrying allele OsSYL2(AA) increased by 5.71% compared with that of the isogenic line carrying allele OsSYL2(CC) in an F(1) hybrid seed production field. The allele frequencies of OsSYL3(AA) and OsSYL2(AA) decreased gradually with an increase in latitude in the Northern Hemisphere. Our results should facilitate the improvement in stigma characteristics of parents of hybrid rice. John Wiley and Sons Inc. 2020-10-30 2020-12 /pmc/articles/PMC7821000/ /pubmed/33031564 http://dx.doi.org/10.1111/tpj.15013 Text en © 2020 The Authors. The Plant Journal published by Society for Experimental Biology and John Wiley & Sons Ltd This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Articles
Dang, Xiaojing
Yang, Yang
Zhang, Yuanqing
Chen, Xiangong
Fan, Zhilan
Liu, Qiangming
Ji, Jie
Li, Dalu
Li, Yanhui
Fang, Bingjie
Wu, Zexu
Liu, Erbao
Hu, Xiaoxiao
Zhu, Shangshang
She, Dong
Wang, Hui
Li, Yulong
Chen, Siqi
Wu, Yufeng
Hong, Delin
OsSYL2 (AA), an allele identified by gene‐based association, increases style length in rice (Oryza sativa L.)
title OsSYL2 (AA), an allele identified by gene‐based association, increases style length in rice (Oryza sativa L.)
title_full OsSYL2 (AA), an allele identified by gene‐based association, increases style length in rice (Oryza sativa L.)
title_fullStr OsSYL2 (AA), an allele identified by gene‐based association, increases style length in rice (Oryza sativa L.)
title_full_unstemmed OsSYL2 (AA), an allele identified by gene‐based association, increases style length in rice (Oryza sativa L.)
title_short OsSYL2 (AA), an allele identified by gene‐based association, increases style length in rice (Oryza sativa L.)
title_sort ossyl2 (aa), an allele identified by gene‐based association, increases style length in rice (oryza sativa l.)
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7821000/
https://www.ncbi.nlm.nih.gov/pubmed/33031564
http://dx.doi.org/10.1111/tpj.15013
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